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Diss Factsheets
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EC number: 200-957-6 | CAS number: 76-39-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Litchefield and Wilsoxon
- Version / remarks:
- J. Pharm, 96 99-113, 1949
- Principles of method if other than guideline:
- Method similar to OECD testing guideline.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2-methyl-2-nitropropan-1-ol
- EC Number:
- 200-957-6
- EC Name:
- 2-methyl-2-nitropropan-1-ol
- Cas Number:
- 76-39-1
- Molecular formula:
- C4H9NO3
- IUPAC Name:
- 2-methyl-2-nitropropan-1-ol
- Details on test material:
- Purity is above 99%.
Constituent 1
- Specific details on test material used for the study:
- NMP Crystals, 2-nitro-2-methyl-1-propanol, 99.6% purity CAS Number 76-39-1
Test animals
- Species:
- rat
- Strain:
- other: Cox-SD albino
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Test animals were male and female Cox-SD albino rats, weighing 200 +/- 25 g. Animals were fasted prior to dosing.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- Test material was delivered by gavage as a single dose. Animals were fasted prior to dosing.
- Doses:
- Dose solution was prepared on a weight/volume basis in sterilized deionized water. The volume of the dosing soluition was adjusted according to the dose and the body weight of the animal; Dosing levels for males: 402; 570;800;1100;1600;2300 mg/kg. Dosing levels for females: 800; 1100; 1310; 1600; 2300 mg/kg
- No. of animals per sex per dose:
- 10 male and 10 female
- Control animals:
- yes
- Details on study design:
- Following single dose by gavage animals were obserbed frequently on the day of dosing and daily thereafter for 14 days. Signs of mortality and morbidity were noted during this period. Animals dying during the observation period were necropsied on the same day or the next day if the animal died during the night. At the end of the 14 days surviving animals were weighed, sacrificed and examined for gross pathology. The oral LD50 was estmated according to the method of Litchfield and Wilcoxon.
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 1 480 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 1 370 - < 1 598
- Key result
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 845 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 710 - < 1 150
- Mortality:
- Male rats: there was 1 death at 570 mg/kg; 5 deaths at 800 mg/kg; 8 deaths at 1100 mg/kg; 9 deaths at 1600 mg/kg and 10 deaths at 2300 mg/kg
Female rats: there were 4 deaths at 1310mg/kg; 7 deaths at 1600 mg/kg and 10 deaths at 2300 mg/kg - Clinical signs:
- At 800 mg/kg male rats were lethargic and ataxic; at higher dose levels male rats were prostrate and had labored respiration;
Female rats at the two highest doses were prostrate and 7/10 showed mild hematuria. - Gross pathology:
- Some evidence of mottled liver in male rats in three highest dose level. All organs were normal for female rats
Applicant's summary and conclusion
- Conclusions:
- The acute oral LD50 in the male rat was 845 (710-1150) mg/kg and in the female rat was 1480 (1370-1598) mg/kg, leading to the conclusion that the substance was more toxic to male than female rats.
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