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EC number: - | CAS number: 20126-76-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
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- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Endpoint summary
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 Jun - 1 Jul 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Draft OECD Guideline 442E (In Vitro Skin Sensitisation: human Cell Line Activation Test)
- Version / remarks:
- December 2015
- Deviations:
- yes
- Remarks:
- cytotoxicity measurement and estimation of the CV75 value was performed by XTT test instead of flow cytometry
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hess. Ministerium für Umwelt, Klimaschutz, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany
- Type of study:
- activation of dendritic cells
Test material
- Reference substance name:
- (1R)-1-isopropyl-4-methyl-cyclohex-3-en-1-ol
- Cas Number:
- 20126-76-5
- Molecular formula:
- C10H18O
- IUPAC Name:
- (1R)-1-isopropyl-4-methyl-cyclohex-3-en-1-ol
Constituent 1
In vitro test system
- Details on the study design:
- TEST CELL LINE
- Strain: THP-1 cells (human monocytic leukemia cell line)
- Source: American Type Culture Collection
- Passage number: 4 (XTT test); 11 (h-CLAT)
CELL CULTURE CONDITIONS
- Type and identity of media: RPMI-1640 supplemented with 10% (v/v) FBS, 100 U/mL penicillin, 100 µg/mL streptomycin, 0.05 mM 2-mercaptoethanol, 4.5 g/L glucose, 1% (v/v) pyruvate and 1% (v/v) L-glutamine
- Temperature (°C): 37 ± 1.5
- CO2 (%): 5.0 ± 0.5
DOSE FINDING ASSAY BY CYTOTOXICITY MEASUREMENT (XTT TEST)
The doses investigated in the main experiment (h-CLAT) were determined with a XTT test. The XTT test is a method to investigate the cytotoxicity of a test substance. The test is based on the cleavage of the yellow tetrazolium salt, sodium 3'-(1-phenylaminocarbonyl)-(3,4-tetrazolium)-bis-(4–methoxy-6-nitro)-benzenesulfonic acid hydrate (XTT), to form an orange water soluble formazan dye by dehydrogenase activity in active mitochondria. Two independent cytotoxicity experiments were performed with different cell cultures to obtain a reliable concentration showing 75% cell viability (CV75).
CONTROLS
Negative Control
- Substance: culture medium
Vehicle control
- Substance: dimethyl sulfoxide (DMSO)
- Concentration: 0.2 - 0.5%
EXPOSURE CONDITIONS
- Exposure duration: 24 ± 1 h
TEST CONCENTRATIONS
- Justification for top dose: The test substance was soluble in DMSO up to and including 125 µg/mL as tested by a solubility test.
0.98, 1.96, 3.91, 7.81, 15.63, 31.25, 62.5 and 125 µg/mL
NUMBER OF REPLICATIONS: quadruplicates per concentration in two independent experiments
MEASUREMENT
- Device: microplate reader (Versamax Molecular Devices)
- Wavelength: 450 nm
- Reference wavelength: 690 nm
EVALUATION CRITERIA
A decrease in number of living cells results in a decrease in the overall activity of mitochondrial dehydrogenases in the sample. This decrease directly correlates to the amount of orange formazan formed, as monitored by the absorbance. The relative absorbance as compared to the solvent control is calculated using the formula:
Relative absorbance = 100 x [(mean absorbance test substance) - (absorbance blank)] / [(mean absorbance vehicle control) - (absorbance blank)]
To calculate the concentration of test substance needed to reduce the relative absorbance to 75% of the vehicle control (CV75) the following formula is used:
CV75 = Conc. > 75 - ([(Conc. > 75) - (Conc. < 75)] x [(% > 75) - 75] / [(% > 75) - (% < 75)])
a) Conc. > 75: max. measured concentration with the % of vehicle control > 75%
b) Conc. < 75: min. measured concentration with the % of vehicle control < 75%
c) % > 75: relative absorbance at a) in %
d) % < 75: relative absorbance at b) in %
ACCEPTANCE CRITERIA
The XTT test is considered to be acceptable if it meets the following criteria:
- mean absorbance of the negative control is ≥ 0.5
- mean viability of the vehicle control is ≥ 70%
h-CLAT (main study)
The h-CLAT method is an in vitro method which quantifies changes of cell surface marker expression (CD86 and CD54) on a human cell line, THP-1 cells, following 24 h exposure to the test substance. The changes of surface marker expression are measured by flow cytometry following cell staining with fluorescein isothiocyanate (FITC)-labelled antibodies. The relative fluorescence intensity of surface markers compared to vehicle control are calculated and used in the prediction model to support the discrimination between sensitisers and non-sensitisers.
CONTROLS
Negative control
- Substance: culture medium
Vehicle control
- Substance: dimethyl sulfoxide (DMSO)
- Concentration: 0.2%
Positive control
- Substance: 2,4-dinitrochlorobenzene (DNCB) in DMSO
- Concentration: 2 and 3 µg/mL
EXPOSURE CONDITIONS
- Exposure duration: 24 ± 1 h
TEST CONCENTRATIONS
- Justification for top dose: Since a CV75 value could not be determined in the XTT test, the highest soluble test substance concentration was multiplied with 1.2 (150 µg/mL). Due to a calculation error the highest test substance concentration used was 300 μg/mL instead of 150 μg/mL.
83.7, 100.5, 120.6, 144.7, 173.6, 208.2, 250 and 300 µg/mL
NUMBER OF REPLICATIONS: at least in triplicates for the different stainings in two independent experiments
STAINING
- Antibodies: FITC anti-CD54 (Clone: Fun-1); FITC anti-CD86 (Clone: 6.5B5); FITC mouse IgG1 (isotype control)
- Temperature: 2 - 8 °C
- Duration: 30 ± 5 min
MEASUREMENT
- Device: flow cytometer FACSCalibur (Becton Dickinson)
EVALUATION CRITERIA
The relative fluorescence intensity (RFI) is used as an indicator of CD86 and CD54 expression, and is calculated as follows for each concentration:
RFI (%) = [(MFI of test substance treated cells) - (MFI of test substance treated isotype control cells) / (MFI of vehicle control cells) - (MFI of vehicle isotype control cells)] x 100
MFI: geometric mean fluorescence intensity (GeoMean)
The cell viability is calculated as follows for each concentration:
Cell viability (%) = (mean cytotox of vehicle control cells) / (mean cytotox of test substance treated cells) x 100
Where the mean cytotox is the mean of GeoMean (7-AAD) isotype control, GeoMean (7-AAD) CD54 and GeoMean (7-AAD) CD86.
The test substance is tested in 2 independent runs. If the RFI of CD86 is ≥ 150% or if the RFI of CD54 is ≥ 200% in both independent run data, the test substance is considered to be a sensitizer. Otherwise it is considered to be a non-sensitizer. In case of different results in both runs, a third run has to be performed. If the RFI of CD86 is ≥ 150% at any dose in at least 2 of 3 independent run data, or if the RFI of CD54 is ≥ 200% in at least 2 of 3 independent run data, the test substance is considered to be a sensitizer. Otherwise it is considered to be a non-sensitizer.
ACCEPTANCE CRITERIA
The study is considered as valid, if the following criteria are met:
- Cell viability of negative control is adjusted to 100% and the cell viability of the vehicle control should be more than 90% in comparison to the negative control.
- In the positive control, RFI values of both CD86 and CD54 should exceed the positive criteria (CD86 ≥ 150% and CD54 ≥ 200%) and the cell viability should be > 50%.
- In the vehicle control, RFI values compared to the negative control of both CD86 and CD54 should not exceed the positive criteria (CD86 ≥ 150% and CD54 ≥ 200%).
- For negative and vehicle controls, the MFI ratio of CD86 and CD54 to isotype control should be > 105%.
- For the test substance resulting in negative outcome, the cell viability at the 1.2 × CV75 value should be less than 90%. If the cell viability at the 1.2 × CV75 value is more than 90% for a positive tested test substance, the data will be acceptable. If 5 mg/mL in saline, 1 mg/mL in DMSO or the highest soluble dose will be used as the maximal test concentration instead of CV75-based dose, the data for test substance are accepted independent by the cell viability.
- The cell viability of at least 4 doses in each experiment should be ≥50%.
Results and discussion
In vitro / in chemico
Resultsopen allclose all
- Key result
- Run / experiment:
- other: 24 h incubation
- Parameter:
- other: relative fluorescence intensity of CD54 (%)
- Value:
- 200
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: in 2 independent runs
- Key result
- Run / experiment:
- other: 24 h incubation
- Parameter:
- other: relative fluorescence intensity of CD86 (%)
- Value:
- 150
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: in 2 independent runs
- Other effects / acceptance of results:
- ADDITIONAL INFORMATION ON CYTOTOXICITY:
XTT test
Cytotoxic effects were not observed following incubation with the test substance up to the highest soluble concentration (125 µg/mL). The viability of the cells spaced within a range of 93.05 to 115.64% in the first experiment and within a range of 89.48 to 111.90% in the second experiment (threshold of cytotoxicity: < 75%). Due to the lack of cytotoxicity, a CV75 value could not be calculated.
h-CLAT
The cell viability was not negatively affected (viability > 95%) in both independent experiments.
ACCEPTANCE OF RESULTS:
- Acceptance criteria met for vehicle control: In the vehicle control, RFI values compared to the negative control of both CD86 and CD54 did not exceed the positive criteria (CD86 ≥150% and CD54 ≥200%).
- Acceptance criteria met for positive control: The RFI values of the positive control for CD86 and CD54 exceeded the positive criteria (CD86 >150% and CD54 >200%) and the cell viability was >50%.
Any other information on results incl. tables
Table 1: Results of the first XTT test
|
Concentration [µg/mL] |
Mean absorbance* |
Blank |
Absorbance in % of vehicle control** |
Negative control |
- |
0.799 ± 0.026 |
0.274 |
97.20 |
Vehicle control |
- |
0.804 ± 0.024 |
0.264 |
100.00 |
Test substance |
0.98 |
0.773 ± 0.034 |
0.259 |
95.28 |
1.96 |
0.785 ± 0.032 |
0.260 |
97.32 |
|
3.91 |
0.780 ± 0.039 |
0.262 |
95.87 |
|
7.81 |
0.727 ± 0.023 |
0.221 |
93.71 |
|
15.63 |
0.762 ± 0.019 |
0.259 |
93.05 |
|
31.25 |
0.823 ± 0.108 |
0.255 |
105.08 |
|
62.5 |
0.813 ± 0.034 |
0.256 |
103.00 |
|
125 |
0.879 ± 0.063 |
0.254 |
115.64 |
*: mean absorbance (absolute) of 4 wells
**: relative absorbance
Table 2: Results of the second XTT test
|
Concentration [µg/mL] |
Mean absorbance* |
Blank |
Absorbance in % of vehicle control** |
Negative control |
- |
0.784 ± 0.005 |
0.265 |
99.11 |
Vehicle control |
- |
0.795 ± 0.030 |
0.271 |
100.00 |
Test substance |
0.98 |
0.739 ± 0.013 |
0.265 |
90.43 |
1.96 |
0.762 ± 0.014 |
0.261 |
95.62 |
|
3.91 |
0.730 ± 0.023 |
0.261 |
89.48 |
|
7.81 |
0.783 ± 0.031 |
0.267 |
98.43 |
|
15.63 |
0.781 ± 0.008 |
0.265 |
98.54 |
|
31.25 |
0.756 ± 0.019 |
0.264 |
93.88 |
|
62.5 |
0.771 ± 0.014 |
0.261 |
97.38 |
|
125 |
0.848 ± 0.043 |
0.262 |
111.90 |
*: mean absorbance (absolute) of 4 wells
**: relative absorbance
Table 3. Results of the first h-CLAT run
|
Concentration [µg/mL] |
Antibody / ISO |
MFI GeoMean (FITC) |
MFI - ISO |
Csto(Geo) GeoMean (7-AAD) |
Mean Cyto |
RFI (%) |
Viability (%) |
Negative control |
- |
ISO |
2.34 |
- |
2.13 |
2.0 |
- |
100.0 |
CD54 |
2.82 |
0.48 |
2.08 |
100.0 |
||||
CD86 |
3.41 |
1.07 |
1.90 |
100.0 |
||||
Vehicle control |
- |
ISO |
2.36 |
- |
2.22 |
2.0 |
- |
100.0 |
CD54 |
2.89 |
0.53 |
1.99 |
100.0 |
||||
CD86 |
3.62 |
1.26 |
1.81 |
100.0 |
||||
Positive control |
2 |
ISO |
3.06 |
- |
2.82 |
2.3 |
- |
86.1 |
CD54 |
6.80 |
3.74 |
2.25 |
705.7* |
||||
CD86 |
8.51 |
5.45 |
1.92 |
432.5* |
||||
3 |
ISO |
3.09 |
- |
3.03 |
2.3 |
- |
87.9 |
|
CD54 |
9.70 |
6.61 |
1.95 |
1247.2* |
||||
CD86 |
9.44 |
6.35 |
1.87 |
504.0* |
||||
Test substance |
83.7 |
ISO |
2.61 |
- |
2.34 |
2.1 |
- |
97.3 |
CD54 |
4.45 |
1.84 |
1.91 |
347.2* |
||||
CD86 |
3.86 |
1.25 |
1.94 |
99.2 |
||||
100.5 |
ISO |
2.75 |
- |
2.41 |
2.1 |
- |
95.4 |
|
CD54 |
4.80 |
2.05 |
1.96 |
386.8* |
||||
CD86 |
4.27 |
1.52 |
1.94 |
120.6 |
||||
120.6 |
ISO |
2.80 |
- |
2.45 |
2.1 |
- |
96.2 |
|
CD54 |
5.62 |
2.82 |
1.91 |
532.1* |
||||
CD86 |
4.54 |
1.74 |
1.90 |
138.1 |
||||
144.7 |
ISO |
2.69 |
- |
2.17 |
1.8 |
- |
110.7 |
|
CD54 |
6.71 |
4.02 |
1.64 |
758.5* |
||||
CD86 |
5.34 |
2.65 |
1.63 |
210.3* |
||||
173.6 |
ISO |
2.89 |
- |
2.22 |
1.9 |
- |
107.5 |
|
CD54 |
7.79 |
4.90 |
1.66 |
924.5* |
||||
CD86 |
5.37 |
2.48 |
1.72 |
196.8* |
||||
208.3 |
ISO |
3.04 |
- |
2.50 |
1.9 |
- |
103.6 |
|
CD54 |
11.33 |
8.29 |
1.68 |
1564.2* |
||||
CD86 |
6.68 |
3.64 |
1.63 |
288.9* |
||||
250 |
ISO |
3.45 |
- |
2.34 |
1.9 |
- |
108.3 |
|
CD54 |
13.01 |
9.56 |
1.62 |
1803.8* |
||||
CD86 |
8.13 |
4.68 |
1.60 |
371.4* |
||||
300 |
ISO |
3.08 |
- |
2.74 |
2.1 |
- |
96.3 |
|
CD54 |
14.80 |
11.72 |
1.72 |
2211.3* |
||||
CD86 |
7.64 |
4.56 |
1.79 |
361.9* |
*: RFI value of CD86 or CD54 exceeded the criteria for a positive response (CD86 ≥ 150% and CD54 ≥ 200%).
Table 4. Results of the second h-CLAT run
|
Concentration [µg/mL] |
Antibody / ISO |
MFI GeoMean (FITC) |
MFI - ISO |
Csto(Geo) GeoMean (7-AAD) |
Mean Cyto |
RFI (%) |
Viability (%) |
Negative control |
- |
ISO |
2.28 |
- |
2.35 |
2.2 |
- |
100.0 |
CD54 |
2.94 |
0.66 |
2.19 |
100.0 |
||||
CD86 |
3.86 |
1.58 |
1.98 |
100.0 |
||||
Vehicle control |
- |
ISO |
2.22 |
- |
2.39 |
2.1 |
- |
100.0 |
CD54 |
2.96 |
0.74 |
2.24 |
100.0 |
||||
CD86 |
4.09 |
1.87 |
1.52 |
100.0 |
||||
Positive control |
2 |
ISO |
2.42 |
- |
2.78 |
2.4 |
- |
87.2 |
CD54 |
7.23 |
4.81 |
2.29 |
650.0* |
||||
CD86 |
6.42 |
4.00 |
1.98 |
213.9* |
||||
3 |
ISO |
2.61 |
- |
3.25 |
2.3 |
- |
89.8 |
|
CD54 |
8.83 |
6.22 |
1.83 |
840.5* |
||||
CD86 |
9.39 |
6.78 |
1.77 |
362.6* |
||||
Test substance |
83.7 |
ISO |
2.34 |
- |
2.42 |
2.1 |
- |
100.0 |
CD54 |
4.74 |
2.40 |
1.89 |
324.3* |
||||
CD86 |
4.34 |
2.00 |
1.84 |
107.0 |
||||
100.5 |
ISO |
2.35 |
- |
2.39 |
2.0 |
- |
102.2 |
|
CD54 |
5.14 |
2.79 |
1.81 |
377.0* |
||||
CD86 |
4.52 |
2.17 |
1.82 |
116.0 |
||||
120.6 |
ISO |
2.45 |
- |
2.46 |
2.0 |
- |
103.2 |
|
CD54 |
6.70 |
4.25 |
1.69 |
574.3* |
||||
CD86 |
4.75 |
2.30 |
1.81 |
123.0 |
||||
144.7 |
ISO |
2.57 |
- |
2.48 |
2.1 |
- |
97.3 |
|
CD54 |
8.01 |
5.44 |
1.68 |
735.1* |
||||
CD86 |
4.43 |
1.86 |
2.16 |
99.5 |
||||
173.6 |
ISO |
2.49 |
- |
2.59 |
2.1 |
- |
98.6 |
|
CD54 |
10.61 |
8.12 |
1.60 |
1097.3* |
||||
CD86 |
4.75 |
2.26 |
2.05 |
120.9 |
||||
208.3 |
ISO |
2.48 |
- |
2.61 |
2.0 |
- |
103.4 |
|
CD54 |
14.71 |
12.23 |
1.47 |
1652.7* |
||||
CD86 |
5.09 |
2.61 |
1.87 |
139.6 |
||||
250 |
ISO |
2.43 |
- |
2.61 |
1.9 |
- |
105.7 |
|
CD54 |
19.27 |
16.84 |
1.39 |
2275.7* |
||||
CD86 |
5.61 |
3.18 |
1.82 |
170.1* |
||||
300 |
ISO |
2.44 |
- |
2.63 |
1.9 |
- |
105.9 |
|
CD54 |
17.12 |
14.68 |
1.41 |
1983.8* |
||||
CD86 |
5.65 |
3.21 |
1.77 |
171.7* |
*: RFI value of CD86 or CD54 exceeded the criteria for a positive response (CD86 ≥150% and CD54 ≥200%).
Applicant's summary and conclusion
- Interpretation of results:
- other: activation of dendritic cells positive according to OECD 442E
- Conclusions:
- Under the conditions of the Human Cell Line Activation Test the test substance increased the expression of both cell surface markers, CD54 and CD86, which is associated with the process of activation of monocytes and dendritic cells. Based on this result, the h-CLAT prediction is considered positive for skin sensitisation.
- Executive summary:
The test substance increased the expression of cell surface marker CD54 and CD86 to greater than 200% and 150%, respectively in two independent experiments. Thus, the h-CLAT prediction for activation of dendritic cells is considered positive.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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