1,1,1,3,3,3-hexafluoropropan-2-ol

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

7 May - 18 Jun 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: Circular on Test Methods of New Chemical Substances (Japan)

Deviations:

no

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

1,1,1,3,3,3-hexafluoropropan-2-ol

EC Number:

213-059-4

EC Name:

1,1,1,3,3,3-hexafluoropropan-2-ol

Cas Number:

920-66-1

Molecular formula:

C3H2F6O

IUPAC Name:

1,1,1,3,3,3-hexafluoropropan-2-ol

Radiolabelling:

no

Sampling and analysis

Details on sampling:

- Sampling intervals/frequency for test organisms: Week 2, 3, 4 and 6
- Sampling intervals/frequency for test medium samples: twice per week
- Sample storage conditions before analysis: no storage
- Details on sampling and analysis of test organisms and test media samples: Test media and 2 fish were sampled for each concentration during the uptake phase. Sample preparation: 1) test media: NaCl and diethylether were added, cooled and shaken. The diethylether phase was used for GC-MS. 2) fish samples: After measurement of weight and length, and homogenization, urea and zeolite were added to distill. The first distilled fraction was collected in diethylether and NaCl was added. The diethylether phase was collected for GC-MS.

Test solutions

Vehicle:

no

Details on preparation of test solutions, spiked fish food or sediment:

Stock solution: 400 mg/L and 40 mg/L

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Koi
- Source: Fish farm, Yashiro-city, Japan
- Length at study initiation: 9.4 cm (mean)
- Weight at study initiation: 21.3 g (mean)
- Lipid content at test initiation: 4.0% (mean)
- Health status: 24 hrs Oxytetracyclin pre-treatment under static condition
- Feeding during test: yes
- Food type: mixture raising feed for young fish
- Amount: 2% body weight
- Frequency: twice a day

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

6 wk

Test conditions

Test temperature:

25 ± 2 °C

Dissolved oxygen:

5.2 - 6.7 mg/L (first concentration)
5.7 - 7.1 mg/L (second concentration)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass, 100 L
- Type: open
- Type of flow-through: propotional diluter
- Renewal rate of test solution: stock solution : test water = 2 mL/min : 800 mL/min
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 1
- No. of vessels per control / vehicle control (replicates): 0

RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: 48 h acute toxicity test in fish (Medaka)
- Results used to determine the conditions for the definitive study: LD 50 for 48 h was 270 mg/L

Nominal and measured concentrations:

Nominal concentration: 1 and 0.1 mg/L
Measured concentration: 1.01 - 1.04 and 0.0961 - 0.0992 mg/L

Reference substance (positive control):

no

Results and discussion

Bioaccumulation factor

Bioaccumulation factor 1

Conc. / dose:

1 mg/L

Type:

BCF

Value:

>= 1.1 - <= 1.4 L/kg

Basis:

whole body w.w.

Calculation basis:

steady state

Bioaccumulation factor 2

Conc. / dose:

0.1 mg/L

Type:

BCF

Value:

>= 1.3 - <= 2.7 L/kg

Basis:

whole body w.w.

Calculation basis:

steady state

Details on results:

- Mortality of test organisms: no mortality
- Behavioural abnormalities: no abnormalities

Any other information on results incl. tables

Table 1. Measured concentration of the test substance in test water (mg/L)

	2 weeks	3 weeks	4 weeks	6 weeks
1^stconcentration group	1.04	1.04	1.03	1.01
2^ndconcentration group	0.0992	0.0974	0.0962	0.0961

 

 

Table 2. Bioconcentration factor (BCF) in fish

	2 weeks	3 weeks	4 weeks	6 weeks
1^stconcentration group	1.3	1.4	1.1	1.2
	1.2	1.2	1.1	1.1
2^ndconcentration group	1.3	2.7	1.7	1.5
	1.7	2.1	1.3	1.7