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Diss Factsheets
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EC number: 202-268-6 | CAS number: 93-69-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
- Species / strain / cell type:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Positive controls:
- yes
- Remarks:
- The following mutagenic substances were used as positive controls: 6.2.1 4-Nitro1,2-phenylene diamine 4-Nitro-1,2-phenylene diamine, C6H7N3〇2; CAS-No.: 99-56-9
- Details on test system and experimental conditions:
- General Preparation: Per strain and dose, four plates with and four plates without S9 mix were used. The test item solutions were prepared and membrane filtered in order to accomplish sterility.
Top agar basis was melted in a microwave oven, after melting 10 mL of histidine-biotin-solution 0.5mMol per 100 mL basis was added and the bottle was placed in the water bath at 43°C.
Plate incorporation method: the following materials were slightly vortexed in a test tube and poured onto the selective agar plates:
• 100 µL test suspension at each dose level, solvent (negative control) or reference mutagen solution (positive control)
• 500 µL S9 mix or phosphate buffer
• 100 µL bacteria suspension
• 2200 µL overlay agar
The plates were closed and left to harden for a few minutes, then inverted and placed in the dark incubator at 37°C.
Pre incubation method: The following materials were gently vortexed in a test tube and incubated at 37°C for 20 minutes:
• 100 µL test suspension at each dose level, solvent (negative control) or reference mutagen solution (positive control)
• 500 µL S9 mix or phosphate buffer
• 100 µL bacteria suspension
After pre-incubation 2000µL overlay agar (top agar) was added, the tube was gently vortexed and the mixture was poured onto the selective agar plate. - Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
The test item Casamid 710 is considered as "not mutagenic under the conditions of the test".
Reference
The test item Casamid 710 is considered as "not mutagenic under the conditions of the test".
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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