Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 220-474-4 | CAS number: 2778-42-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1981
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- 1,3-bis(1-isocyanato-1-methylethyl)benzene
- EC Number:
- 220-474-4
- EC Name:
- 1,3-bis(1-isocyanato-1-methylethyl)benzene
- Cas Number:
- 2778-42-9
- Molecular formula:
- C14H16N2O2
- IUPAC Name:
- 1,3-bis(1-isocyanato-1-methylethyl)benzene
- Details on test material:
- - Name of test material (as cited in study report): 85,772
- Lot/batch No.: S-13708-76
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: New Zealand White, H.A.R.E. Rabbits For Research, Hewitt, N.J.
- Housing: Individually in wire mesh suspension cages
- Diet (e.g. ad libitum): Certified Rabbit Chow 5322, Ralston Purina Co., ad libitum
- Water (e.g. ad libitum): Potable water, ad libitum
ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12 h dark/ 12 h fluorescent light
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: Left eye served as untreated control
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL - Duration of treatment / exposure:
- Single treatment
- Observation period (in vivo):
- 14 d; eye irritation scored on Days 1, 2, 3, 4, 7, 10 and 13
- Number of animals or in vitro replicates:
- Nine
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): Eyes in three rabbits rinsed with sterile water for 60 sec
- Time after start of exposure: 30 sec
SCORING SYSTEM: Draize method
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 15.1
- Max. score:
- 110
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other: with washout
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 13.6
- Max. score:
- 110
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other: without washout
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Remarks:
- Mean of 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- not specified
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- iris score
- Basis:
- mean
- Remarks:
- mean of 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- not specified
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Remarks:
- mean of 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- not specified
- Remarks on result:
- other:
- Remarks:
- With washout
- Irritation parameter:
- iris score
- Basis:
- mean
- Remarks:
- Mean of 6 animals
- Time point:
- 24/48/72 h
- Score:
- 0
- Reversibility:
- not specified
- Remarks on result:
- other:
- Remarks:
- With washout
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 2.6
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 2.3
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 1.67
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #4
- Time point:
- 24/48/72 h
- Score:
- 2.6
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #5
- Time point:
- 24/48/72 h
- Score:
- 2
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #6
- Time point:
- 24/48/72 h
- Score:
- 2.67
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: 7
- Time point:
- 24/48/72 h
- Score:
- 1.67
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- With washout
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: 8
- Time point:
- 24/48/72 h
- Score:
- 2
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- With washout
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: 9
- Time point:
- 24/48/72 h
- Score:
- 2
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- With washout
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 3.3
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 3.3
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 3.3
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- chemosis score
- Basis:
- animal #4
- Time point:
- 24/48/72 h
- Score:
- 3
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- chemosis score
- Basis:
- animal #5
- Time point:
- 24/48/72 h
- Score:
- 3
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- chemosis score
- Basis:
- animal #6
- Time point:
- 24/48/72 h
- Score:
- 3.67
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- Without washout
- Irritation parameter:
- chemosis score
- Basis:
- animal: 7
- Time point:
- 24/48/72 h
- Score:
- 2.67
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- With washout
- Irritation parameter:
- chemosis score
- Basis:
- animal: 8
- Time point:
- 24/48/72 h
- Score:
- 2
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- With washout
- Irritation parameter:
- chemosis score
- Basis:
- animal: 9
- Time point:
- 24/48/72 h
- Score:
- 3.67
- Reversibility:
- not fully reversible within: 13 d
- Remarks on result:
- other:
- Remarks:
- With washout
- Irritant / corrosive response data:
- Irritation to the conjunctivae appeared to dissipate but still persisted at the termination of the study.
For details, see 'table 1' and 'table 2' - Other effects:
- - No corneal damage at any time
- All treated eyes responded to light and no iridal score was observed in any rabbit
- Discharge, chemosis and redness of the conjunctivae were observed in all animals
- Rinsing appeared to lessen the irritation
Any other information on results incl. tables
Table 1. Ocular irritation (Draize) scores (mean)
Group |
No |
Washed |
1 |
2 |
3 |
4 |
7 |
10 |
13 |
1 |
6 |
No |
18.0 |
15.3 |
12.0 |
13.0 |
13.3 |
13.0 |
11.7 |
2 |
3 |
Yes |
15.3 |
13.3 |
12.0 |
13.3 |
12.7 |
12.0 |
10.7 |
Table 2: Mean ocular irritation scores observed at 24, 48 and 72 h
Animal No. | Corneal opacity | Iritis | Conjunctivae | Chemosis |
1 | 0 | 0 | 2.6 | 3.3 |
2 | 0 | 0 | 2.3 | 3.3 |
3 | 0 | 0 | 1.67 | 3.3 |
4 | 0 | 0 | 2.6 | 3 |
5 | 0 | 0 | 2 | 3 |
6 | 0 | 0 | 2.67 | 3.67 |
7* | 0 | 0 | 1.67 | 2.67 |
8* | 0 | 0 | 2 | 2 |
9* | 0 | 0 | 2 | 3.67 |
* Eyes in three rabbits rinsed with sterile water for 60 sec
Applicant's summary and conclusion
- Interpretation of results:
- Category 2A (irritating to eyes)
- Conclusions:
- Under the test conditions, the test material was considered to be irritating to eyes in rabbit.
- Executive summary:
A study was conducted to evaluate the ocular toxicity of the test substance in rabbits. The study was performed similar to the OECD Guideline 405. 0.1 mL of the test substance was instilled into the right eye of nine rabbits; the left eye served as an untreated control. One group of six rabbits received no further treatment. In the second group of three rabbits, eyes were rinsed 30 sec post-instillation for 60 sec. Irritation was scored by Draize scoring system at 1, 2, 3, 4, 7, 10 and 13 d post-instillation. Mean irritation scores at 24, 48 and 72 h for animals in group 1 (eyes without washing) were 0/0/2.6/3.3; 0/0/2.3/3.3; 0/0/1.67/3.3; 0/0/2.6/3.0; 0/0/2/3.0 and 0/0/2.67/3.67. Mean irritation scores at 24, 48 and 72 h for animals in group 2 (eyes with washing) were 0/0/1.67/2.67; 0/0/2/2 and 0/0/2/3.67. Irritation to the conjunctivae appeared to dissipate but still persisted at the termination of the study. Mean overall irritation score for 24, 48 and 72 h was 15.1/110 and 13.6/110 in the washed and unwashed eyes, respectively (Chow, 1981).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)