p-phenylenebis(methylamine)

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-12

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

1984

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Batch No.: not specific
Purity: not specific

Analytical monitoring:

no

Details on sampling:

The test item be directly dispersed in culture medium. Samples were taken at 0, 24, 48 and 72hours and the absorbance measured at 665nm using a Jenway 6100 Spectrophotometer.

Vehicle:

no

Details on test solutions:

The test item be directly dispersed in culture medium.

Test organisms (species):

Scenedesmus sp.

Test type:

static

Water media type:

other: culture medium

Limit test:

no

Total exposure duration:

72 h

Test temperature:

24 °C

pH:

8.0

Nominal and measured concentrations:

3.125, 6.25, 12.5, 25 and 50 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass conical flask (250 mL) containing 100 mL test solution and loosely stoppered to reduce evaporation. All flask were incubated and shaken (approximately 100 rpm) in an orbital shaker.
- Initial cells density: 15500 cells/mL
- Control end cells density: 262000 cells/mL
- No. of vessels per concentration (replicates): 3

OTHER TEST CONDITIONS
- Adjustment of pH: 8
- Photoperiod: continuous ( approximately 7000 lux)

TEST CONCENTRATIONS
- Test concentrations: 3.125, 6.25, 12.5, 25 and 50 mg/L

Reference substance (positive control):

not specified

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

12 mg/L

Conc. based on:

test mat.

Basis for effect:

biomass

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

14 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

6.25 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

The data mean cell density of control at 0 h is 15500 cells/mL, mean cell density of control at 72h is 262000 cell/mL. The data show that cell concentration of control cultures increased by factor of 17 during the test in line with the OECD Guideline that status the environment must be at least by a factor of 16 after 72 hours.
All test and control cultures were inspected microscopically at 72 hours. There were no abnormalities detected in any of the control test cultures.

Validity criteria fulfilled:

yes

Conclusions:

For test item, the EbC50 (72h) is 12 mg/L. The ErC50 (0-24h) is 14 mg/L.

Executive summary:

The study was performed to assess the growth inhibition of test item to Scenedesmus subspicatus according to the OECD Guideline for Testing of Chemicals No. 201 (2006).

All test and control cultures were inspected microscopically at 72 hours. There were no abnormalities detected in any of the control test cultures. For test item, the EbC50 (72h) is 12 mg/L. The ErC50 (0-24h) is 14 mg/L.

Description of key information

One read-across study is available.

For test item, the EbC50 (72h) is 12 mg/L. The ErC50 (0-24h) is 14 mg/L.

Key value for chemical safety assessment

EC50 for freshwater algae:

14 mg/L

Additional information

One read-across study is available which was performed to assess the growth inhibition of test item to Scenedesmus subspicatus according to the OECD Guideline 201.

All test and control cultures were inspected microscopically at 72 hours. There were no abnormalities detected in any of the control test cultures. For test item, the EbC50 (72h) is 12 mg/L. The ErC50 (0-24h) is 14 mg/L.