Diisobutyl adipate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

1984

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Dibutyl adipate

EC Number:

203-350-4

EC Name:

Dibutyl adipate

Cas Number:

105-99-7

Molecular formula:

C14H26O4

IUPAC Name:

dibutyl adipate

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

Stock solution was prepared with DMSO (100 mg/l). Controls with and without this vehicle were taken for the test.

Test organisms

Test organisms (species):

Selenastrum sp.

Details on test organisms:

Selenastrum capricornutum ATCC 22662

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Nominal and measured concentrations:

The EC50 values were calculated based on 5 nominal concentrations (1-5 mg/l).

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

ca. 2.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Details on results:

EC50 (72h) = 2.8 mg/l
NOEC = 2.0 mg/l

Any other information on results incl. tables

TOXICITY TO AQUATIC PLANTS e.g. Algae

Species: Selenastrum capricornutum ATCC 22662

End-point: Biomass [X]; Growth rate [ ]; Other [ ]

Exposure period: 72 hours

Results: Biomass: EC50 (24h) =

EC50 (72h) = 2.8 mg/l

NOEC = 2.0 mg/l

LOEC =

Analytical monitoring: Yes [ ] No [X] ? [ ]

Method: open-system [X]; closed-system [ ]

OECD Test Guideline 201 (1984)

GLP: Yes [ ] No [X] ? [ ]

Test substance: Dibutyl adipate, purity = 99 %

Remarks: The EC50 values were calculated based on 5 nominal concentrations

(1-5 mg/l). Stock solution was prepared with DMSO (100

mg/l). Controls with and without this vehicle were taken for the test.

Reference: EA, Japan (1994)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes