O-isobutyl ethylthiocarbamate

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vivo

Type of information:

other: published data

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Dithiocarbamates are related compounds to Thionocarbamate.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Materials and methods

Objective of study:

metabolism

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EPA OPP 85-1 (Metabolism and Pharmacokinetics)

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method B.36 (Toxicokinetics)

Deviations:

yes

Remarks:

Distribution was not investigated.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ziram

EC Number:

205-288-3

EC Name:

Ziram

Cas Number:

137-30-4

IUPAC Name:

zinc bis(dimethyldithiocarbamate)

Test material form:

solid: compact

Details on test material:

Dithiocarbamates are related compounds to Thionocarbamate.
- Name of test material (as cited in study report): Ziram
-IUPAC Name zinc bis dimethyldithiocarbamate
- Lot no.: 14C-Ziram: 027F9221
Nonradiolabeled Ziram: Technical
- Purity: 14C-Ziram: 96.9%
Nonradiolabeled Ziram: 99%
- Description: Creamy white powder
-Molecular formula:C6H12N2S4Zn
-Molecular weight range :305.8419
-SMILES notation :CN(C)C(=S)S[Zn]SC(=S)N(C)
-InChI=1/2C3H7NS2.Zn/c2*1-4(2)3(5)6;/h2*1-2H3,(H,5,6);/q;;+2/p-2/rC6H12N2S4Zn/c1-7(2)5(9)11-13-12-6(10)8(3)4/h1-4H3

Radiolabelling:

yes

Remarks:

14C-Ziram

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

- Source: Charles River, US
- Age at study initiation: 44 days (m); 51 days (f)
- Weight at study initiation: 149-169 g (m); 145-162 g (f)

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Duration and frequency of treatment / exposure:

Single and multiple (15 days daily) application

Doses / concentrations

Remarks:

Doses / Concentrations:
Single: 15 and 352 mg/kg
Multiple: 15 mg/kg (14x nonradiolabeled; 1x radiolabeled)

No. of animals per sex per dose / concentration:

5

Control animals:

yes, concurrent vehicle

Details on dosing and sampling:

- Tissues and body fluids sampled: air, urine, faeces, blood, several organs

The CO2 trapping solution and the volatile traps were collected at 0-4, 4-8, 8-12, and 12-24 h following administration of the radiolabeled Ziram and daily thereafter for a total of 4 days. Urine and faeces samples were collected at 0-6, 6-12, and 12-24 hours after the radiolabeled dose and daily thereafter for a total of 7 days. Urine and faeces were collected in plastic containers surrounded by ice. At the end of the collection period, the animals were anesthetized with halothane and exsanguinated by cardiac puncture. Blood (2 to 5 mL) was collected and weighed in heparinized tubes and saved for radioanalysis. After sacrifice the cages were washed with a 1.0% trisodium phosphate solution which was saved for analysis.

Results and discussion

Toxicokinetic / pharmacokinetic studies

Details on absorption:

The mean 14C recovery ranged from 79% to 92% of the total doses administered.

Details on distribution in tissues:

The mean total radioactivity retained in the tissues and carcasses ranged from 1.11% to 1.92% of the total dose administered. For the low dose groups, the residue levels in the blood and tissues ranged from 0.05 to 2.5 ppm (µg 14C-Ziram equivalents/g sample). The highest levels were found in blood, liver, kidney, heart, lungs, spleen and thyroid gland.

Details on excretion:

The majority of the radioactivity was found in urine (17% to 35%), faeces (9% to 18%), and expired air (36% to 53%). The rate of elimination was relatively fast; the majority of the radioactivity was eliminated within 48 hours after dosing.
No apparent sex-related differences were observed for 14C elimination or distribution for any of the treated groups.

Metabolite characterisation studies

Metabolites identified:

yes

Applicant's summary and conclusion

Conclusions:

Interpretation of results : no bioaccumulation potential based on study results
Dithiocarbamates are related compounds to Thionocarbamate .
The mean total radioactivity retained in the tissues and carcasses ranged from 1.11% to 1.92% of the total dose administered. For the low dose groups, the residue levels in the blood and tissues ranged from 0.05 to 2.5 ppm (µg 14C-Ziram equivalents/g sample). The highest levels were found in blood, liver, kidney, heart, lungs, spleen and thyroid gland.