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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 - 25 Jan 2001
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reliability 1

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guidelineopen allclose all
according to guideline
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
according to guideline
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Potassium 1,1,2,2,3,3,4,4,4-nonafluorobutane-1-sulphonate
EC Number:
EC Name:
Potassium 1,1,2,2,3,3,4,4,4-nonafluorobutane-1-sulphonate
Cas Number:
Molecular formula:
potassium 1,1,2,2,3,3,4,4,4-nonafluorobutane-1-sulfonate
Specific details on test material used for the study:
- Source and lot/batch No.of test material: L-7038 Lot 2
- Purity: 97.3%
- Expiration date of the lot/batch: 17 Jan 2002
- Storage condition of test material: Ambient room temperature
- Stability under test conditions: Stable

Sampling and analysis

Analytical monitoring:
Details on sampling:
Details on sampling
- Concentrations: All; sampled at test initiation, 24 hours, and at test termination.
- Sampling method: not described
- Sample storage conditions before analysis: Samples were prepared for analysis on the day the samples were collected, and stored within autosampler vials. Samples were analyzed on the day collected, or the following day.

Test solutions

Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stirring of primary stock (4000 mg a.i./L) by inversion and and stirring. Dilution of primary stock with dilution water to obtain lower concentrations.
- Controls: Blank test medium
- Evidence of undissolved material: All solutions appeared clear and colorless.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Cladoceran; Water flea
- Source: Cultures maintained by the test laboratory with known history
- Age at study initiation: < 24 hours, from parental daphnids of more than two weeks old, which were observed to have no neonates present less than 24 hours prior to test initiation
- Culturing: Adult daphnids were cultured in water from the same source and at approximately the same temperature as used during the test. Adult daphnids in the cultures were held for at least 14 days prior to collection of the juveniles for testing. The adults showed no signs of disease or stress during the holding period. During the 14-day period preceding the test, water temperatures ranged from 19.1 to 20.2°C. The pH of the water ranged from 8.2 to 8.5, and dissolved oxygen ranged from 8.0 to 8.6 mg/L. Daphnids in the cultures were fed a mixture of yeast, Cerophyl®, and trout chow, as well as a suspension of the freshwater green alga, Selenastrum capricornutum. The adults were fed prior to test initiation, but neonates were not fed during the test.
- Food type: Not fed during test

Study design

Test type:
Water media type:
Limit test:
Total exposure duration:
48 h

Test conditions

132 - 136 mg CaCO3/L
Test temperature:
19.5 - 20.5 °C, from continuous measurement during the test
7.9 - 8.5
Dissolved oxygen:
8.3 - 8.6 mg/L
290 - 295 µmhos/cm
Nominal and measured concentrations:
Nominal: 0 mg/L, 250 mg/L, 500 mg/L, 1000 mg/L, 2000 mg/L, 4000 mg/L
Measured: < LOQ, 234 mg/L, 470 mg/L, 886 mg/L, 1707 mg/L, 3767 mg/L
Details on test conditions:
- Test vessel: 300-mL polyethylene beakers 225-mL fill volume, depth ca. 5.5 cm
- Aeration: dilution water aerated prior to filling.
- No. of organisms per vessel: 10
- No. of vessels per concentration: two
- No. of vessels per control: two
- Source/preparation of dilution water: 40-m deep well located at testing lab. Water was passed through
a sand filter and pumped into a storage tank and aerated with spray nozzles. Water was filtered to 0.45
µm prior to testing. Periodic water analysis, Attachments 1 and 2.
- Pesticides: all < LOQ
- Alkalinity: 184 mg/L as CaCO3
- Ca/Mg ratio: 2.6 (mass basis)
- Culture medium different from test medium: No
- Intervals of water quality measurement: most recent analysis on samples collected Oct 14 and 15, 1999.
- Adjustment of pH: No
- Photoperiod: 16 h:8h with 30-min transitions
- Light intensity: Colortone ® 50 fluorescent lights, 144 and 143 lux at surface of representative chamber at test initiation and termination, respectively
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality and immobilization at approximately 4 h, 24h, and 48 h
- Spacing factor for test concentrations: 2x
- Range finding study: Yes, no further details provided
- Results used to determine the conditions for the definitive study: Yes
Reference substance (positive control):

Results and discussion

Effect concentrations
Key result
48 h
Dose descriptor:
Effect conc.:
2 183 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
act. ingr.
Basis for effect:
The study reported dead and immobilized daphnids separately. For the purpose of calculating the EC50, these effects are combined.
Remarks on result:
other: 95% CI, 1707 - 3767 mg a.i./L
Details on results:
- Mobility: See Table 2
- Mobility of control: At 48 hours, one daphnid was immobilized (5%), all other control organisms appeared normal during the test.
Reported statistics and error estimates:
EC50 and 95% CI calculated using binomial probability method with nonlinear interpolation.

Any other information on results incl. tables

Table 2, Cumulative percent mortality/immobility and treatment-related effects

Mean measured concentration (mg/L) Cumulative # Immobilized and/or Dead/ # Originally Exposed (Observations)¹ Cumulative # Immobilized and/or Dead/ # Originally Exposed (Observations)¹ Cumulative # Immobilized and/or Dead/ # Originally Exposed (Observations)¹ Cumulative percent immobilized (and/or Dead)
  4 hours 24 hours 48 hours  
Negative Control 0 / 20
(20 AN)
0 / 20
(20 AN)
1 / 20
(19 AN, 1 D)
234 0 / 20
(20 AN)
0 / 20
(20 AN)
1 / 20
(19 AN, 1M)
470 0 / 20
(20 AN)
0 / 20
(20 AN)
0 / 20
(20 AN)
886 0 / 20
(20 AN)
0 / 20
(20 AN)
1 / 20
(19 AN, 1 M)
1707 0 / 20
(20 AN)
0 / 20
(20 AN)
4 / 20
(16 AN, 3 D, 1 M)
3767 20 / 20
(20 D)
20 / 20
(20 D)
20 / 20
(20 D)

¹ Observations: AN = Appears Normal; D = Dead; M = Immobilized

Applicant's summary and conclusion

Validity criteria fulfilled:
the validity criteria of OECD TG 202 were fulfilled. Control immobilization was < 10% at the end of the test. The dissolved oxygen concentration was = 3 mg/L in the control and test vessels at the end of the test.
The 48-hour EC50 of PFBSK+ to Daphnia magna was 2183 mg/L (EPA 850.1010 and OECD TG 202).
Executive summary:

The 48-hour EC50 of PFBSK+ to Daphnia magna was examined in a static test conducted according to EPA 850.1010 and OECD TG 202. Analytically determined concentrations were < LOQ, 234 mg/L, 470 mg/L, 886 mg/L, 1707 mg/L, and 3767 mg/L. No statistically significant levels of immobilization were observed at 234, 470, and 886 mg/L; 20% cumulative immobilization was observed at 1707 mg/L, and 100% cumulative immobilization was observed at 9433 mg/L. The 48-hour EC50 is 2183 mg/L.

The test was conducted according to internationally accepted test guidelines and was GLP compliant. It is reliable without restriction and suitable for Risk Assessment, Classification and Labeling, and PBT Analysis.