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EC number: 277-086-3 | CAS number: 72928-60-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2018-04-02 to 2018-04-05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Trihydrogen [29H,31H-phthalocyaninetrisulphonato(5-)-N29,N30,N31,N32]cuprate(3-), compound with N,N'-di(o-tolyl)guanidine (1:3)
- EC Number:
- 277-086-3
- EC Name:
- Trihydrogen [29H,31H-phthalocyaninetrisulphonato(5-)-N29,N30,N31,N32]cuprate(3-), compound with N,N'-di(o-tolyl)guanidine (1:3)
- Cas Number:
- 72928-60-0
- Molecular formula:
- C32H16-τN8Cu(SO3C15H18N3)τ
- IUPAC Name:
- Bis and tris and tetra [N-(2-methylanilino)-N’-(2-methylanilino) methaniminium][phthalocyaninesulfonato-κ4N29,N30,N31,N32]cuprate(II)
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
At test facility: 2017-04-19 to 2017-04-27: refrigerator (6 ± 2 °C)/ since 2017-04-27: room temperature, protected from light, in the tightly closed original container; since 2017-11-02 additionally in evacuated desiccator
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
* 0 mg/L of test item (control)
* 0.0500 mg/L of test item
* 0.158 mg/L of test item
* 0.500 mg/L of test item
* 1.58 mg/L of test item
* 5.00 mg/L of test item
* 15.8 mg/L of test item
- Sampling method: Sampling done in freshly prepared media at the start of exposure (0 hours) and in old media at the end of exposure (48 hours) without daphnids.
The control and the lower concentrated samples (0.0500 mg/L and 0.158 mg/L) were analyzed enriched factor 10.
The samples 0.500 mg/L and 1.58 mg/L were analyzed undiluted. The higher concentrated samples (5.00 mg/L and 15.8 mg/L) were diluted with dilution medium prior to analysis.
- Sample storage conditions before analysis: All samples were stored at 6 ± 2 °C until the start of the analysis, if necessary. Prepared samples were stored in the autosampler at room temperature until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution with a nominal test item concentration of 15.8 mg/Lwas freshly prepared with dilution water one day prior to the start of the exposure (at day -1). This solution was stirred with a magnetic stirrer at approximately 1100 rpm for 24 hours at room temperature.
The four other concentrations: 0.0500 - 0.158 - 0.500 - 1.58 - 5.00 mg/L of test item were prepared by appropriate dilution of the stock solution.
- Controls: 4 replicates (without test item)
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnid
- Strain/clone: STRAUSS
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
- Age of parental stock (mean and range, SD): less than 24 hours (at test initiation)
- Feeding during test: The daphnids were not fed during the study.
ACCLIMATION
- Acclimation period: No acclimation period as the daphnids were cultured under the same conditions as the test replicates.
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus provided at libitum
- Feeding frequency: at least 5 times per week
- Health during acclimation (any mortality observed): A COMPLETER
Study design
- Test type:
- static
- Water media type:
- other: medium Elendt M4 according to OECD 202, Annex 3 (2004)
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 18 - 22°C
- pH:
- 8.2 ±0.8
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity
- Type : closed with watch glasses
- Material, size, headspace, fill volume: 250mL sterile glass Erlenmeyer flasks, filled at 20 mL.
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): no renewal
- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): 4 replicates
- No. of vessels per control (replicates): 4 replicates
OTHER TEST CONDITIONS
- Adjustment of pH: yes, for the culture medium
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity: Diffuse light, maximum of 1500 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
A COMPLETER
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations:
* 0 mg/l of test item (control)
* 0.0500 mg/l of test item
* 0.5000 mg/l of test item
* 5.00 mg/l of test item
- Results used to determine the conditions for the definitive study: Immobilization rates were used to determine the conditions for the definitive study - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 11.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI 10.1 – 13.1
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- ca. 5.89 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI 5.00 – 6.71
- Duration:
- 24 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 15.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 15.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 15.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 15.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: No mortality observed
- Results with reference substance (positive control):
- - Results with reference substance valid?
Yes
- ECx: EC50 at 2.00 mg/l
- 95% confidence limits: 1.00 - 4.00 mg/L - Reported statistics and error estimates:
- EC10 and EC 50 are determined via the following the Sigmoidal dose-response (variable slope) following the equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC50-X)*HillSlope))
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the nominal concentrations of the test item, the 48-hour EC50 for Daphnia magna was 11.4 mg/L. No effects on Daphnia magna STRAUS were observed.
- Executive summary:
In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test item were determined at the test facility according to OECD 202 (2004) from 2018-04-02 to 2018-04-05, with the definitive exposure phase from 2018-04-03 to 2018-04-05.
The study was conducted under static conditions over a period of 48 hours with the following nominal test item concentrations: 0.0500 - 0.158 - 0.500 - 1.58 - 5.00 - 15.8 mg/L of the test item.
The test item concentration was blue coloured in a concentration-related manner and visually clear throughout the exposure.
Twenty daphnids (divided into 4 replicates with 5 daphnids each) were exposed to each test item concentration and the control.
The concentrations of the test item were analytically verified via HPLC-DAD in fresh media at the start (0 hours) and in old media at the end of the exposure (48 hours) in each test item concentration and in the control.
The measured concentrations of the test item were between 88 and 105% of the nominal test item concentration at 0 hours and between 93 and 103% at 48 hours, respectively.
The measured test item concentrations remained stable within ± 20% of the nominal limit concentration of the test item over the test period.
The validity criteria of the test guideline were fulfilled.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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