2-Butanone, 1,3,4-trihydroxy-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29-09-2003

Reliability:

1 (reliable without restriction)

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Version / remarks:

ISO International Standard 6341 : 'Water quality — Determination of the inhibition of the mobility of Daphnia magna Straus — Acute toxicity test, Third edition, 1996-04-01.

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

OECD guideline No. 202 Part l: "Daphnia sp., Acute Immobilisation Test", Adopted April 4, 1984.

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

EEC directive 92/69, Part C: Methods for the determination of ecotoxicity, Publication No. L383, December 1 992, C.2. "Acute Toxicity for Daphnia"

Deviations:

no

Principles of method if other than guideline:

NA

GLP compliance:

yes

Specific details on test material used for the study:

NA

Analytical monitoring:

yes

Details on sampling:

Frequency: at t=0h and t=48h
Volume: 10 mL from the approximate centre of the test vessel

Vehicle:

yes

Details on test solutions:

Preparation of test solutions started with stock solutions in test medium at 100 mg/l. No special treatment other than a magnetic stirring period of ca. 20 minutes was necessary to completely dissolve the test substance in the test medium. The lower test concentrations were prepared by subsequent dilution of the stock in test medium. All final test solutions were clear and colourless
Nominal test concentrations: 0.1 , 1 .0, 10 and 100 mg/l.
Blank-control Test medium without test substance or other additives (O mg/l).

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
For the test selection of young daphnia with an age of < 24 hours, from parental daphnids of more than two weeks old.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

NA

Post exposure observation period:

NA

Hardness:

250 mg/l expressed as CaC03 and the pH: 8.0 ± 0.2 after aeration.

Test temperature:

The temperature of the test medium was 20.5 °C at the start of the test. The temperature continuously measured in a temperature control vessel varied between 20.2 and 20.7°C during the test, and complied with the requirements as laid down in the protocol (18-22°C, constant within 2°C).

pH:

pH: 6.0-8.5

Dissolved oxygen:

oxygen: >7 mg/l at the start, 25 mg/l at the end of the test

Salinity:

NA

Conductivity:

NA

Nominal and measured concentrations:

Analysis of the samples taken during the test showed that the measured concentration was in agreement with nominal (97-99%).

Details on test conditions:

NA

Reference substance (positive control):

yes

Remarks:

potassium dichromate (K2Cr207)

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Under the conditions of the present study (S)-1,3,4-trihydroxybutan-2-one did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC). Erythruolose did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC).

Results with reference substance (positive control):

The 24h-EC50 of potassium dichromate was 1.2 mg/l with a 95% confidence interval between 1.0 and 1.5 mg/l.
The 48h-EC50 of potassium dichromate was 0.60 mg/l with a 95% confidence interval between 0.52 and 0.76 mg/l.

Reported statistics and error estimates:

NA

No remarks

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present study (S)-1,3,4-trihydroxybutan-2-onedid not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC). (S)-1,3,4-trihydroxybutan-2-one did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC).

Executive summary:

Under the conditions of the present study (S)-1,3,4-trihydroxybutan-2-one did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC). Erythruolose did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC).

Description of key information

Under the conditions of the present study (S)-1,3,4-trihydroxybutan-2-one did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC). (S)-1,3,4-trihydroxybutan-2-one did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L