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EC number: 470-780-9 | CAS number: 55312-97-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 Aug - 07 Dec 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- 20.5 mg of the test item were dissolved in Acetonitrile and made up with Acetonitrile to the mark in a 100 mL volumetric flask to prepare a stock solution of 205 mg/L. Defined volumes of this stock solution were diluted with Milliporewater to obtain standard solutions in the range of 0.041 to 4.10 mg/L.
Although the overall method sensitivity may be better, the concentration of the lowest used calibration solution is employed as the limit of quantification.
These solutions were used to calibrate the HPLC-system. - Vehicle:
- no
- Details on test solutions:
- To produce the only test concentration 105.0 mg of the test item were added to 1 litre of dilution water, treated for 1 hour in an ultrasonic bath and then stirred for 24 h on a magnetic stirrer. Finally undissolved particles of the test item were removed by filtration using a folded filter of pore size 7-12 µm.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Common name: water flea
Source: Strain of Bundesgesundheitsamt Berlin
Maintenance and Acclimatisation:
A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- The hardness of the dilution water used was 15.4 °dH (= 274.89 mg/L CaCO3).
- Test temperature:
- 20.2 °C
- pH:
- 8.0
- Dissolved oxygen:
- 8.8 mg/L
- Nominal and measured concentrations:
- nominal: 100 mg/L
measured: 99.1 mg/L (0 h), 98.8 mg/L (48 h) - Details on test conditions:
- Test vessels: 50 ml glass beakers holding 10 neonates in 20 mL of test medium
Experimental design: 1 test concentration plus 1 control1; 0 neonates per vessel, 2 replicates per concentration/control; no feeding during the exposure period;
Photoperiod: 16 h light: 8 h dark
Aeration: none
Criteria of effects: The criterion of adverse effects used in this study was the item-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No toxic effects against daphnia at a test concentration of 100 mg/1. The results are expressed in terms of nominal concentrations. Recovery rates correspond to 99.1% of nominal values at 0 hours, and to 98.8% of nominal values at 48 hours, respectively.
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Conclusions:
- No toxic effects against daphnia at a test concentration of 100 mg/1. The results are expressed in terms of nominal concentrations. Recovery rates correspond to 99.1% of nominal values at 0 hours, and to 98.8% of nominal values at 48 hours, respectively.
- Executive summary:
A study was performed to assess the acute toxicity to Daphnia magna STRAUS under static conditions.
The study was conducted in accordance with the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test' (adopted April 13, 2004) which is in most parts equivalent to the EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (OJ. No. L383A, 29.12.92) Part C, Method 2 Acute toxicity for Daphnia'.
The Daphnia were exposed to a limit test concentration of nominally 100 mg/L test item dissolved in water. Auxiliaries used to prepare the test media were an ultrasonic bath and a magnetic stirrer.
Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined:Time [h] EC 50 [mg/L] 24 > 100 48 > 100 No toxic effects against daphnia at a test concentration of 100 mg/L.
The results are expressed in terms of nominal concentrations. Recovery rates correspond to 99.1% of nominal values at 0 hours, and to 98.8% of nominal values at 48 hours, respectively.
Reference
Validity criteria for the measurement of the daphnia toxicity:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
Young daphnids, aged less than 24 hours at the start of the test, are exposed to the test substance at a range of concentrations for a period of 48 h. | Age of daphnids used: neonates Exposure time: 48 h | Yes |
In the control, including the control containing the solubilising agent, not more than 10 % of the daphnids should have been immobilized. (Not more than 10 % of the control daphnids should show immobilisation or other signs of disease or stress, for example, discoloration or unusual behaviour such as trapping at surface of water) | In the controls, no immobilisation of the daphnids occured. | Yes |
Test concentrations: At least five test concentrations should be used. They should be arranged in a geometric series with a separation factor preferably not exceeding 2.2. The highest concentration tested should preferably result in 100 % immobilization, and the lowest concentration tested should preferably give no observable effect. | Test was a limit test (conc. used was 100 mg/L). | Not applicable |
Description of key information
No toxic effects against daphnia at a test concentration of 100 mg/1.The results are expressed in terms of nominal concentrations. Recovery rates correspond to 99.1% of nominal values at 0 hours, and to 98.8% of nominal values at 48 hours, respectively.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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