(1α,2α,5α)-2,2,6-trimethylbicyclo[3.1.1]heptan-2-ol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

February 13, 2003 to September 11, 2003

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Concentrations: 0 mg/L (control), 13, 22, 36, 60, and 100 mg/L

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):
A 100 mg/L stock solution was prepared by bringing 0.1004 g of test substance to 1,000 mL with dilution water in a Class A volumetric flask. The solution was mixed on a magnetic stirrer for approximately 2 hours. A series of solutions was then prepared by bringing appropriate volumes of stock solution to 250 mL with dilution water in Class A volumetric flasks.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Age at study initiation (mean and range, SD): less than 24 hours old
- Weight at study initiation (mean and range, SD): average wet weight of 0.18 mg
-Method of breeding: in-house culture
- Source: Aquatic Biosystems Inc., Fort Collins, Colorado, on February 20, 2003
- Feeding during test: no

ACCLIMATION
- Acclimation period: 35 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: freshwater algae, Selenastrum capricornutum, and a mixture of yeast and trout chow
- Feeding frequency: once each day

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

160 to 180 mg/L as CaCO3

Test temperature:

20.0 to 21.0°C (mean = 20.6°C)

pH:

7.0

Dissolved oxygen:

from 7.7 to 8.8 mg/L (mean = 8.4 mg/L)

Conductivity:

600 μmhos/cm

Nominal and measured concentrations:

Nominal concentrations were: 0 mg/L (control), 13, 22, 36, 60, and 100 mg/L.
Mean measured concentrations were: ND (none detected at or above the limit of quantitation; control), 12.2, 20.0, 33.5, 56.7, and 93.6 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: 40 mL
- Type: closed
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: carbon filtered, deionized water collected at T.R. Wilbury Laboratories in Marblehead, Massachusetts. Water was adjusted to a hardness of 160 to 180 mg/L as CaCO3 and stored in polyethylene tanks where it was aerated and recirculated through particle filters, activated carbon, and an ultraviolet sterilizer. Prior to use, the pH of the dilution water was adjusted to <8.0 with 5% H3PO4.
- Total organic carbon: ND
- Pesticides: ND

OTHER TEST CONDITIONS
- Photoperiod: 16 hour light and 8 hour dark
- Light intensity: 32 footcandles

RANGE-FINDING STUDY
- Test concentrations: 10 mg/L and 100 mg/L
- Results used to determine the conditions for the definitive study: 100% survival in the control, 100% survival at 10 mg/L, and 20% survival at 100 mg/L.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

58.5 mg/L

95% CI:

51.1 - 66.6

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

72.8 mg/L

95% CI:

56.7 - 93.6

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

33.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: 0
- Immobilisation of control: 0

Conclusions:

The EC50 of the test item on D. magna was calculated as 58.5 mg/L after 48 h.

Executive summary:

The accute toxicity of the test item on the aquatic invertebrate Daphnia magna was studied. The test followed the OECD guideline 202, 1984. It was perfomed during 48 hours under static conditions and closed vessels. The test animals were less than 24 hours old. A total of 20 animals were used per each test concentration as it is stated in the guideline. The mean measured concentrations utilized were 12.2, 20.0, 33.5, 56.7, and 93.6 mg/L. Which ranged from 91 to 95% of nominal concentrations. The effective concentrations calculated were a EC50 of 58.5 mg/L, a LC50 of 72.8 mg/L, and a NOEC of 33.5 mg/L

Description of key information

EC50 (48 h)= 58.5 mg/L (Daphnia magna, OECD 202)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

58.5 mg/L

Additional information

The accute toxicity of the test item on the aquatic invertebrate Daphnia magna was studied. The test followed the OECD guideline 202, 1984. It was perfomed during 48 hours under static conditions and closed vessels. The test animals were less than 24 hours old. A total of 20 animals were used per each test concentration as it is stated in the guideline. The mean measured concentrations utilized were 12.2, 20.0, 33.5, 56.7, and 93.6 mg/L. Which ranged from 91 to 95% of nominal concentrations. The effective concentrations calculated were a EC50 of 58.5 mg/L, a LC50 of 72.8 mg/L, and a NOEC of 33.5 mg/L