N,N-dimethylacrylamide

Neurotoxicity

Administrative data

Endpoint:

neurotoxicity: sub-chronic oral

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable publication which meets basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

Neurotoxicity of Acrylamide and Related Compounds and Their Effects on Male Gonads in Mice

Author:

Hashimoto K. Sakamoto J, Tanii H

Year:

1981

Bibliographic source:

Arch Toxicol (1981) 47: 179-189

Materials and methods

Test guideline

Qualifier:

no guideline required

GLP compliance:

not specified

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N,N-dimethylacrylamide

EC Number:

220-237-5

EC Name:

N,N-dimethylacrylamide

Cas Number:

2680-03-7

Molecular formula:

C5H9NO

IUPAC Name:

N,N-dimethylacrylamide

Test animals

Species:

mouse

Strain:

other: ssY

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 5-6 weeks
- Weight at study initiation: 29±2.2 g
- Housing: 5-6 per plastic cage, containing wooden flakes
- Diet: laboratory chow, ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: 0.9% saline

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

10 weeks

Frequency of treatment:

Twice weekly

Doses / concentrations

Remarks:

Doses / Concentrations:
1.7 mmol/kg
Basis:
actual ingested

No. of animals per sex per dose:

5 (without metabolic activation)
6 (with metabolic activation by phenobarbital)

Control animals:

yes, concurrent vehicle

Details on study design:

Groups of five or six animals were dosed with the test compound, which was dissolved in 0.9% saline solution, with a blunt tip metal intubation needle. Doses were given twice weekly, at 1.7 mmol/kg for 8-10 weeks. Control animals received a comparable volume of the vehicle. To examine the effect of metabolic activation, sodium phenobarbital (PB), which was prepared from phenobarbital before use, was given intraperitoneally at 50 mg/kg for five successive days per week, from one week before, up until the last week of treatment with the test compound.

Examinations

Neurobehavioural examinations performed and frequency:

For the test experiments, only those animals in which rotarod performance was able to be carried out were preliminarily selected. A modified apparatus of Dunham and Miya (1957), which consisted of a 5 cm diameter, roughly surfaced PVC rod, rotated at 3 revolutions per minute, was used. Arithmetic means of the longest performance periods, in five successive 30 s trials in each rat, were calculated.

Results and discussion

Results of examinations

Details on results:

Neurotoxicity
No effects were seen.

Effect of phenobarbitol treatment
No effects were seen.

Effect levels

Dose descriptor:

NOAEL

Basis for effect level:

other: no neurotoxic effects noted

Remarks on result:

not determinable

Remarks:

no NOAEL identified