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EC number: 416-600-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993-11-24 to 1994-10-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- EC 92/69
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Analytical purity: >= 99.65 %
Lot/batch No.: 93.166 - Analytical monitoring:
- yes
- Details on sampling:
- Stock solutions of the desired strength are prepared by dissolving the test article in deionized water.The chosen test concentrations will be prepared by dilution of the stock solution. If high concentrations are tested, the test article is dissolved in the dilution water directly.
Test medium samples were taken at the start of the test (before introduction of the test animals) and at the end of the test. Samples were immediately deep-frozen and analysed later to determine actual concentrations.
Samples were thawed and filtered through Acrodisc LC 13 PVDF 0.45 µm syringe filter to remove any undissolved test article. - Vehicle:
- yes
- Details on test solutions:
- Formulations of the test article were prepared as follows: Considering the low water solubility of the test article and to prevent impact of solid particles, an "extract" of the test article was prepared. A 1000 mg/L suspension of the test article with the auxiliary substance (DMSO - 50 mg/L) was stirred for 4 hours. The test article in excess was then filtered. The saturated solution obtained was used at the following concentrations:
- preliminary study: 25, 50, 75, 90 and 100 %;
- main study: 10, 50, 75 and 100 %;
100 % extract without auxiliary substance was also tested in the main study. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species, strain: Freshwater microcrustacea, Daphnia magna;
Supplier: Société d'Elevage Piscicole Contrôlé - La Grande Rivière - Saint Forgeux - France;
Age at initiation of treatment. less than 24 hours old; - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- NA
- Hardness:
- 180 mg CaCO3/L
- Test temperature:
- 19.3 - 20.9 °C
- pH:
- 7.5 - 8.0
- Dissolved oxygen:
- 8.4 - 8.6 mg/L
- Salinity:
- NA
- Nominal and measured concentrations:
- A 1000 mg/L suspension of the test article with the auxiliary substance (DMSO - 50 mg/L) was stirred for 4 hours. The test article in excess was then filtered. The saturated solution obtained was used at the following concentrations:
- preliminary study: 25, 50, 75, 90 and 100 %;
- main study: 10, 50, 75 and 100 %;
100 % extract without auxiliary substance was also tested in the main study. - Details on test conditions:
- For each study, twenty daphnies were used per treated group, in comparison with an untreated control group and an auxiliary substance control group. Each group was constituted of four tubes, each containing 5 Daphnia for 10 mL of test medium (one tube contained 6 Daphnia).
Immobilisations were recorded about 24 and 48 hours after the start of the test.
Measurements of pH, dissolved oxygen and temperature were performed at the beginning and at the end of the test for each test medium. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Immobilisations:
The number of immobilisations noted was not proportional to the test article concentration. Only 10 % immobilisation was noted in the high concentration treated group without auxiliary substance (100 % extract).
Observation of the test media:
The extracts tested were colourless and limpid throughout the study.
Analysis of actual concentrations:
No test article could be detected, the actual concentrations being lower than the limit of detection of the analytical method (1 µg/mL).
Quality criteria:
The quality criteria were respected for the study.
Further details: See Sect. "Remarks on results including tables and figures" - Results with reference substance (positive control):
- NA
- Reported statistics and error estimates:
- NA
- Validity criteria fulfilled:
- yes
- Conclusions:
- HAT ISO was tested for acute toxicity to Daphnia magna in a 48 hours static test. The immobilisations observed were not proportional to the test concentration. Thus the EC50/48 hours for Daphnia magna was higher than the limit of solubility. No classification and labelling is required according to Regulation 1272/2008/EC (CLP).
- Executive summary:
HAT ISO was tested for acute toxicity to Daphnia magna in a 48 hours static test.
Immobilisations:
The number of immobilisations noted was not proportional to the test article concentration. Only 10 % immobilisation was noted in the high concentration treated group without auxiliary substance ( 100 % extract).
Observation of test media:
The extracts tested were colourless and limpid throughout the study.
Analysis of actual concentrations:
No test article could be detected, the actual concentrations being lower than the limit of detection of the analytical method (1 µg/mL).
Quality criteria:
The quality criteria were respected for the study:
- No immobilisations were noted in the control and auxiliary control groups.
- No control Daphnia was trapped at the surface of water.
- The oxygen concentration was higher than 2 mg/L.
- Despite no test article could be detected during analysis, any test article dissolved in the media was considered as stable because no other peak of possible degradation products appeared on the chromatograms.
CONCLUSION
The immobilizations observed were not proportional to the test article concentration.
Despite the use of auxiliary solvent is recommended in the Guidelines to improve the solubility of the test article, it may not help the interpretation of the results. DMSO is not toxic to Daphnia at the concentration used, as proved by the auxiliary control group, but it may have contributed to obtain erratic results.
This is the reason why a group of Daphnia was treated without auxiliary solvent, at the maximum concentration of test article. 10 % immobilizations were obtained.
Therefore the results can be expressed as follows:
EC50, for Daphnia magna (nominal concentrations):
Time of exposure (hours)
EC50
Nominal concentration (% extract of a 1 g/l preparation)
24
higher than limit of solubility
up to 100 %
48
higher than limit of solubility
up to 100 %
EC0(no observed effect concentration after 48 hours) : not determined
EC100(lowest concentration causing 100 % immobilization after 48 hours) : not determined
Reference
Quality criteria:
The quality criteria were respected for the study:
- No immobilisations were noted in the control and auxiliary control groups.
- No control Daphnia was trapped at the surface of water.
- The oxygen concentration was higher than 2 mg/L.
- Despite no test article could be detected during analysis, any test article dissolved in the media was considered as stable because no other peak of possible degradation products appeared on the chromatograms.
PRELIMININARY STUDY
Number of Daphnia per group at the start of study: 20
(except 100 % concentration: 21 daphnies).
Nominal Concentration (% extract) |
Cumulated immobilizations |
|||
24 hours |
48 hours |
|||
nb |
% |
nb |
% |
|
0 (Control) |
0 |
0 |
0 |
0 |
Auxiliary control |
0 |
0 |
0 |
0 |
25 % |
0 |
0 |
15 |
75 |
50 % |
0 |
0 |
19 |
95 |
75 % |
1 |
5 |
14 |
70 |
90 % |
1 |
5 |
6 |
30 |
100 % |
1 |
5 |
3 |
14.3 |
nb : number
MAIN STUDY
Number of Daphnia per group at the start of study: 20
(except 100 % concentration: 21 daphnies).
Nominal Concentration (% extract) |
Cumulated immobilizations |
|||
24 hours |
48 hours |
|||
nb |
% |
nb |
% |
|
0 (Control) |
0 |
0 |
0 |
0 |
Auxiliary control |
0 |
0 |
0 |
0 |
10 % |
1 |
5 |
1 |
5 |
50 % |
0 |
0 |
17 |
85 |
75 % |
0 |
0 |
2 |
10 |
100 % |
0 |
0 |
3 |
14.3 |
100 * % |
0 |
0 |
2 |
10 |
* : Without auxiliary substance.
CONCLUSION
The immobilizations observed were not proportional to the test article concentration.
Despite the use of auxiliary solvent is recommended in the Guidelines to improve the solubility of the test article, it may not help the interpretation of the results. DMSO is not toxic to Daphnia at the concentration used, as proved by the auxiliary control group, but it may have contributed to obtain erratic results.
This is the reason why a group of Daphnia was treated without auxiliary solvent, at the maximum concentration of test article. 10 % immobilizations were obtained.
Therefore the results can be expressed as follows:
EC50, for Daphnia magna (nominal concentrations):
Time of exposure (hours) |
EC50 |
Nominal concentration (% extract of a 1 g/l preparation) |
24 |
higher than limit of solubility |
up to 100 % |
48 |
higher than limit of solubility |
up to 100 % |
EC0 (no observed effect concentration after 48 hours) : not determined
EC100 (lowest concentration causing 100 % immobilization after 48 hours) : not determined
Description of key information
EC50 was determined to be higher than the water solubility limit.
Key value for chemical safety assessment
Additional information
HAT ISO was tested for acute toxicity to Daphnia magna in a 48 hours static test.
Immobilisations:
The number of immobilisations noted was not proportional to the test article concentration. Only 10 % immobilisation was noted in the high concentration treated group without auxiliary substance ( 100 % extract).
Observation of test media:
The extracts tested were colourless and limpid throughout the study.
Analysis of actual concentrations:
No test article could be detected, the actual concentrations being lower than the limit of detection of the analytical method (1 µg/mL).
Quality criteria:
The quality criteria were respected for the study:
- No immobilisations were noted in the control and auxiliary control groups.
- No control Daphnia was trapped at the surface of water.
- The oxygen concentration was higher than 2 mg/L.
- Despite no test article could be detected during analysis, any test article dissolved in the media was considered as stable because no other peak of possible degradation products appeared on the chromatograms.
CONCLUSION
The immobilizations observed were not proportional to the test article concentration.
Despite the use of auxiliary solvent is recommended in the Guidelines to improve the solubility of the test article, it may not help the interpretation of the results. DMSO is not toxic to Daphnia at the concentration used, as proved by the auxiliary control group, but it may have contributed to obtain erratic results.
This is the reason why a group of Daphnia was treated without auxiliary solvent, at the maximum concentration of test article. 10 % immobilizations were obtained.
Therefore, the results can be expressed as follows:
EC50, for Daphnia magna (nominal concentrations):
Time of exposure (hours) |
EC50 |
Nominal concentration (% extract of a 1 g/L preparation) |
24 |
higher than limit of solubility |
up to 100 % |
48 |
higher than limit of solubility |
up to 100 % |
EC0 (no observed effect concentration after 48 hours) : not determined
EC100 (lowest concentration causing 100 % immobilization after 48 hours) : not determined
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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