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Diss Factsheets
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EC number: 416-600-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-10-11 to 2001-01-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Analytical purity: 97.4 %
Lot/batch No.: July 30, 1999 - Analytical monitoring:
- not specified
- Details on sampling:
- Preparation of test solutions:
In order to improve the substance dispersing in water, the test substance was ground down to very fine particles using a mortar and pestle before weighing. As the solubility of the test substance in Water is very low, test solutions at 31.6 and 100 mg/L were prepared by direct addition of the test substance in the test vessels. Test solutions at 1, 3.13 and 10 mg/L were prepared by means of a suspension at 20 mg/L in dechlorinated water because test substance quantities necessary for direct addition to the test flasks were too low to be accurately weighed (< 10 mg). The suspension was agitated during 8 minutes. Test solutions (1, 3.16 and 10 mg/L) were prepared immediately by further dilution of the suspension, maintained under agitation, with dechlorinated water. - Vehicle:
- no
- Details on test solutions:
- The test solutions were prepared as follows:
- two control solutions: activated sludge in dechlorinated water to obtain a final sludge concentration of 1.6 g/L,
- five test substance solutions: activated sludge in dechlorinated water (at 1.6 g/L) and test substance (to obtain final concentrations of 1, 3.16, 10, 31.6 and 100 mg/L),
- three reference substance (3,5-dichlorophenol) solutions: activated sludge in dechlorinated water (at 1.6 g/L) and reference substance (to obtain final concentrations of 4, 12 and 36 mg/L). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Type: Activated sludge collected from a water treatment works containing effluent from a predominantly domestic origin;
Origin: The water treatment plant Emereaude (SIARR) (76141 Petit-Quevilly, France); - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- 280 ± 20 mg/L as CaCO3
- Test temperature:
- 20 ± 2 °C
- pH:
- 6.0 - 8.0
- Dissolved oxygen:
- Not indicated
- Salinity:
- Not indicated
- Nominal and measured concentrations:
- Nominal concentrations: 1; 3.16; 10, 31.6; 100 mg/L of test substance; inoculum: 1.6 g/L;
Measured concentrations: Analytical measurement was not performed. - Details on test conditions:
- Environmental conditions during the test:
Temperature: between 20 and 22 °C;
Illumination: no special precautions were taken;
Dilution water: dechlorinated tap water was used, with a hardness of 280 ± 20 mg/L as CaCO3;
Duration of test: 3 hours;
Aeration: air was bubbled through each solution at the rate of 0.5 to 1 L/min until the start of oxygen concentration measurement;
Test item concentrations: 1; 3.16; 10, 31.6; 100 mg/L of test substance; inoculum: 1.6 g/L; - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Validity criteria:
As the difference between the two controls was below 15 % and the EC50 of 3,5-dichlorophenol was between 35 and 30 mg/L, the test was therefore considered valid.
Determination of the EC50:
Respiration rates of the test and reference solutions are presented in the table (See Sect. " Remarks on results incl. tables and figures")
The respiration rate of the test solution of highest concentration (100 mg/L) was equivalent to the respiration of the first control (i.e. these rates were within 15 % of each other). Therefore, the oxygen consumption rate of the for other test solutions (1 to 31.6 mg/L) was not determined.
The EC50, based on nominal concentrations, were as follows:
- Test substance: 3-hour EC50 (mg/L): > 100;
- Reference substance: 3-hour EC50 (mg/L): 10.8 (95 % confidence limits (mg/L): 8.87 - 13.1; - Results with reference substance (positive control):
- Reference substance: 3-hour EC50 (mg/L): 10.8
- Reported statistics and error estimates:
- Reference substance: 3-hour EC50 95 % confidence limits (mg/L): 8.87 - 13.1;
- Validity criteria fulfilled:
- yes
- Conclusions:
- HAT ISO was tested for toxicity to bacteria in a 3-hour activated sludge respiration inhibition test. The EC50 of the test item HAT ISO was found to be > 100 mg/L. HAT ISO is considered as non-toxic for aquatic microorganisms. No classification and labelling for aquatic toxicity is required according to Regulation 1272/2008/EEC (CLP).
- Executive summary:
HAT ISO was tested for toxicity to bacteria in a 3-hour activated sludge respiration inhibition test.
The EC50, based on nominal concentrations, were as follows:
- Test substance: 3-hour EC50 (mg/L): > 100;
- Reference substance: 3-hour EC50 (mg/L): 10.8
(95 % confidence limits (mg/L): 8.87 - 13.1);
HAT ISO is considered as non-toxic for aquatic microorganisms.
Reference
Respiration rates of the test and reference solutions are presented in the table.
Table: Respiration rate in mg O2-L-1.h-1.
|
Concentration (mg/L) |
Respiration rate in mg O2-L-1.h-1. |
Percent inhibition (%) |
Control 1 |
0 |
42.0 |
|
Control 2 |
0 |
45.0 |
|
|
|
|
|
Reference substance |
4 |
34.8 |
20 |
|
12 |
19.5 |
55 |
|
36 |
7.5 |
83 |
|
|
|
|
PATE HAT SECHEE |
1 |
ND |
|
|
3.16 |
ND |
|
|
10 |
ND |
|
|
31.6 |
ND |
|
|
100 |
46.5 |
0 |
|
|
|
|
ND: not determined
Description of key information
HAT ISO was tested for toxicity to bacteria in a 3-hour activated sludge respiration inhibition test.
The EC50, based on nominal concentrations, were as follows:
- Test substance: 3-hour EC50 (mg/L): > 100;
- Reference substance: 3-hour EC50 (mg/L): 10.8
(95 % confidence limits (mg/L): 8.87 - 13.1);
HAT ISO is considered as non-toxic for aquatic microorganisms.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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