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EC number: 238-687-6 | CAS number: 14639-97-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Storage stability and reactivity towards container material
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- pH
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- Endpoint summary
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Endpoint summary
Administrative data
Description of key information
The in vitro skin irritation/corrosion test in the EpiDerm model with Diammonium tetrachlorozincate indicates that the test item is non-corrosive and non-irritant to skin .
Based on the in vitro eye irritation assay in the isolated chicken eyes test with Diammonium tetrachlorozincate, the test item is a severe eye irritant
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 439 (In Vitro Skin Irritation: Reconstructed Human Epidermis Test Method)
- Version / remarks:
- version 28 July 2015
- GLP compliance:
- yes (incl. QA statement)
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- non-transformed keratinocytes
- Cell source:
- skin obtained from plastic surgery from multiple donors
- Vehicle:
- unchanged (no vehicle)
- Details on test system:
- RECONSTRUCTED HUMAN EPIDERMIS (RHE) TISSUE
- Model used: EPISKINTM (SM) (Manufacturer: SkinEthic, France
- Tissue batch number(s):18-EKIN-017
- Expiry Date: 30 April 2018
TEMPERATURE USED FOR TEST SYSTEM
- Temperature used during treatment / exposure: 22.1-24.2 °C
- Temperature of post-treatment incubation (if applicable): 37°C
REMOVAL OF TEST MATERIAL AND CONTROLS
-Volume and number of washing steps: 1wahsing step: rinsing thoroughly with PBS
MTT DYE USED TO MEASURE TISSUE VIABILITY AFTER TREATMENT / EXPOSURE
- MTT concentration: 2 mL of 0.3 mg/mL MTT per well
- Incubation time: 3h
- Spectrophotometer: 96-well plate spectrophotometer
- Wavelength: 570nm
NUMBER OF REPLICATE TISSUES: 3
CONTROL TISSUES USED IN CASE OF MTT DIRECT INTERFERENCE (see any other info on mat and meth) - Control samples:
- yes, concurrent negative control
- yes, concurrent positive control
- yes, concurrent MTT non-specific colour control
- Amount/concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 20 mg
VEHICLE
- Amount(s) applied (volume or weight with unit): na; no formulation was required
NEGATIVE CONTROL: PBS
- Concentration (if solution): 50µl
POSITIVE CONTROL: SDS 5%
- Concentration (if solution): 50µl - Duration of treatment / exposure:
- 15 minutes (± 0.5 min)
- Duration of post-treatment incubation (if applicable):
- 42h
- Number of replicates:
- 3
- Irritation / corrosion parameter:
- % tissue viability
- Value:
- 74.4
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of irritation
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- In this in vitro EPISKINTM (SM) model test with diammonium tetrachlorozincate(2-), the results indicate that the test item is non-corrosive and non-irritant to the skin, UN GHS Classification: No Category.
- Executive summary:
An in vitro skin corrosivity and irritation test of diammonium tetrachlorozincate(2-) was performed in a reconstructed human epidermis model.EPISKINTM(SM) is designed to predict and classify thecorrosivity and irritation potential of chemicals by measuring its cytotoxic effect as reflected in the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The corrosivity and irritation potential of the test item was evaluated according to theOECD No. 431 and No. 439 guidelines.
Disks of EPISKINTM(SM) were treated with the test item and incubated for 15 minutes (irritation testing) and 4 hours (corrosivity testing) at room temperature. Exposure of the test item was terminated by rinsing with Phosphate Buffered Saline (PBS). The epidermis units were then incubated at 37°C for 42 hours in an incubator with 5% CO2,in a > 95% humidified atmosphere (irritation testing). The viability of each disk was assessed by incubating the tissues for 3 hours with MTT solution at 37°C in an incubator with 5% CO2protected from light,in a > 95% humidified atmosphere. The precipitated formazan crystals were then extracted using acidified isopropanol and quantified spectrophotometrically.
Physiological saline (0.9% (w/v) NaCl solution) treated epidermis were used as negative control and glacial acetic acid treated epidermis were used as positive control (two units/control) in case of the corrosivity testing. PBS treated epidermis were used as negative control and 5 % (w/v) Sodium Dodecyl Sulphate (SDS) solution treated epidermis were used as positive control (three units/control) in case of the irritation testing. Two additional disks were used to provide in each case an estimate of colour contribution (NSCliving) from the test item. For each treated tissue, the viability was expressed as a % relative to the negative control. For corrosivity, if the mean relative viability is <35% the test item is considered to be corrosive to skin. For irritation, if the mean relative viability after 15 minutes of exposure and 42 hours post incubation is less or equal (≤) to 50% of the negative control, the test item is considered to be irritant to skin.
Corrosivity testing:
Following exposure with diammonium tetrachlorozincate(2-), the mean cell viability was 81.3% compared to the negative control. This is above the threshold of 35%, therefore the test item was considered as being non-corrosive to skin.
Irritation testing:
Following exposure with diammonium tetrachlorozincate(2-), the mean cell viability was 74.7% compared to the negative control. This is above the threshold of 50%, thereforethe test item was considered as being non-irritant to skin.
In conclusion, in thisin vitroEPISKINTM(SM)model test withdiammonium tetrachlorozincate(2-), the results indicate that the test item is non-corrosive and non-irritant to the skin, UN GHS Classification: No Category.
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Reconstructed Human Epidermis (RHE) Test Method)
- Version / remarks:
- version 29 July 2016
- GLP compliance:
- yes (incl. QA statement)
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- non-transformed keratinocytes
- Cell source:
- skin obtained from plastic surgery from multiple donors
- Vehicle:
- unchanged (no vehicle)
- Details on test system:
- RECONSTRUCTED HUMAN EPIDERMIS (RHE) TISSUE
- Model used: EPISKIN-SM (Manufacturer: SkinEthic, France, Catalogue Number: EPISKIN/S/13
- Tissue batch number(s):18-EKIN-017
- Expiry Date: 30 April 2018
TEMPERATURE USED FOR TEST SYSTEM
- Temperature used during treatment / exposure: 23-24.6°C
- Temperature of post-treatment incubation (if applicable): 37°C
REMOVAL OF TEST MATERIAL AND CONTROLS
-Volume and number of washing steps: 1wahsing step: rinsing thoroughly with PBS
MTT DYE USED TO MEASURE TISSUE VIABILITY AFTER TREATMENT / EXPOSURE
- MTT concentration: 2.2 mL of 0.3 mg/mL MTT
- Incubation time: 3h
- Spectrophotometer: 96-well plate spectrophotometer
- Wavelength: 570nm
NUMBER OF REPLICATE TISSUES:2
CONTROL TISSUES USED IN CASE OF MTT DIRECT INTERFERENCE (see any other info on mat and meth)
PREDICTION MODEL / DECISION CRITERIA (choose relevant statement):
- The test substance is considered to be corrosive to skin, if the mean relative viability after 4 hours of exposure is below 35% of the negative control. - Control samples:
- yes, concurrent negative control
- yes, concurrent positive control
- yes, concurrent MTT non-specific colour control
- Amount/concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 20 mg
VEHICLE
- Amount(s) applied (volume or weight with unit): na; no formulation was required
NEGATIVE CONTROL: NaCI (9 g/l saline)
- Concentration (if solution): 50µl
POSITIVE CONTROL: glacial acetic acid
- Concentration (if solution): 50µl - Duration of treatment / exposure:
- 4h
- Number of replicates:
- 2
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 1
- Value:
- 81.3
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of irritation
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- In this in vitro EPISKINTM (SM) model test with diammonium tetrachlorozincate(2-), the results indicate that the test item is non-corrosive and non-irritant to the skin, UN GHS Classification: No Category.
- Executive summary:
An in vitro skin corrosivity and irritation test of diammonium tetrachlorozincate(2-)was performed in a reconstructed human epidermis model.EPISKINTM(SM) is designed to predict and classify thecorrosivity and irritationpotential of chemicals by measuring its cytotoxic effect as reflected in the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The corrosivity and irritation potential of the test item was evaluated according to theOECD No. 431 and No. 439guidelines.
Disks of EPISKINTM(SM)were treated withthe test itemand incubated for 15 minutes (irritation testing) and4 hours (corrosivity testing)at room temperature. Exposure of the test item was terminated by rinsing with Phosphate Buffered Saline (PBS). Theepidermis units were then incubated at 37°C for 42 hours in an incubator with 5% CO2,in a > 95% humidified atmosphere(irritation testing). The viability of each disk was assessed by incubating the tissues for 3 hours with MTT solution at 37°C in an incubator with 5% CO2protected from light,in a > 95% humidified atmosphere. The precipitated formazan crystals were then extracted using acidified isopropanol and quantified spectrophotometrically.
Physiological saline (0.9% (w/v) NaCl solution) treated epidermis were used as negative control and glacial acetic acid treated epidermis were used as positive control (two units/control) in case of the corrosivity testing. PBS treated epidermis were used as negative control and 5 % (w/v) Sodium Dodecyl Sulphate (SDS) solution treated epidermis were used as positive control (three units/control) in case of the irritation testing. Two additional disks were used to provide in each case an estimate of colour contribution (NSCliving) from the test item. For each treated tissue, the viability was expressed as a % relative to the negative control. For corrosivity, if the mean relative viability is <35% the test item is considered to be corrosive to skin. For irritation, if the mean relative viability after 15 minutes of exposure and 42 hours post incubation is less or equal (≤) to 50% of the negative control, the test item is considered to be irritant to skin.
Corrosivity testing:
Following exposure withdiammonium tetrachlorozincate(2-), the mean cell viability was 81.3% compared to the negative control. This is above the threshold of 35%, therefore the test item was considered as being non-corrosive to skin.
Irritation testing:
Following exposure withdiammonium tetrachlorozincate(2-), themean cell viability was 74.7% compared to the negative control. This is above the threshold of 50%, thereforethe test item was considered as being non-irritant to skin.
In conclusion, in this in vitro EPISKINTM(SM) model test with diammonium tetrachlorozincate(2-), the results indicate that the test item is non-corrosive and non-irritant to the skin, UN GHS Classification: No Category.
Referenceopen allclose all
The results of the optical density (OD) measured at 570 nm of each sample and the calculated relative viability % values are presented below:
Table: Optical Density mean (OD) and the calculated relative viability % of the samples
Substance |
Optical Density (OD) |
Viability (% RV) |
|||
|
Measured |
Blank corrected |
|||
Negative Control: |
1 |
0.837 |
0.790 |
100.0 |
|
Phosphate buffered saline |
2 |
0.835 |
0.788 |
99.9 |
|
|
3 |
0.837 |
0.790 |
100.1 |
|
|
mean |
-- |
0.789 |
100.0 |
|
Positive Control: |
1 |
0.085 |
0.038 |
4.8 |
|
5% (w/v) SDS solution
|
2 |
0.105 |
0.058 |
7.4 |
|
|
3 |
0.094 |
0.047 |
6.0 |
|
|
mean |
-- |
0.048 |
6.0 |
|
Test Item: |
1 |
0.650 |
0.603 |
76.4 |
|
diammonium tetrachlorozincate(2 -) |
2 |
0.622 |
0.575 |
72.9 |
|
|
3 |
0.637 |
0.590 |
74.7 |
|
|
mean |
-- |
0.589 |
74.7 |
Notes:
1. Mean blank value was 0.047.
2. Optical density means the mean value of the duplicate wells for each sample
The results of the optical density (OD) measured at 570 nm of each extract and the calculated % viability of the cells is presented below:
Substance | Optical Density (OD) | Viability | |||||
Measured | Blank corrected | (% RV) | |||||
Negative Control: | 1 | 0.837 | 0.790 | 99.1 | |||
Physiological saline | 2 | 0.852 | 0.805 | 100.9 | |||
(0.9% (w/v) NaCl) | mean | -- | 0.798 | 100.0 | |||
Positive Control: | 1 | 0.049 | 0.002 | 0.3 | |||
Glacial acetic acid | 2 | 0.050 | 0.003 | 0.4 | |||
mean | -- | 0.003 | 0.4 | ||||
Test Item: | 1 | 0.682 | 0.635 | 79.6 | |||
diammonium tetrachlorozincate(2-) | 2 | 0.709 | 0.662 | 83.0 | |||
mean | -- | 0.649 | 81.3 |
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 438 (Isolated Chicken Eye Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage)
- Version / remarks:
- version 09 October 2017
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Vehicle:
- physiological saline
- Controls:
- yes, concurrent vehicle
- yes, concurrent positive control
- yes, concurrent negative control
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied: 30mg - Duration of treatment / exposure:
- 10 seconds
- Number of animals or in vitro replicates:
- not applicable
- Details on study design:
- treatments:
-test substance treated chicken eye: treated with 30 mg diammonium tetrachlorozincate
-positive control chicken eye: treated with 30 mg imidazole
-negative control eye: treated with 30µL physiological saline (0.9% (w/v) NaCl solution
REMOVAL OF TEST SUBSTANCE
- Washing (if done): cornea surface was rinsed thoroughly with 20ml physiological saline
- Time after start of exposure: after 10'' of exposure
SCORING SYSTEM:
The control eye and test eyes were evaluated pre-treatment and at approximately 30, 75, 120, 180 and 240 minutes after the post-treatment rinse.
Minor variations within ±5 minutes were considered acceptable.
The cornea thickness and cornea opacity were measured at all time points.
Fluorescein retention was measured on two occasions, at base line (t=0) and 30 minutes after the post-treatment rinse.
TOOL USED TO ASSESS SCORE: hand-slit lamp / biomicroscope/fluorescein - Irritation parameter:
- percent corneal swelling
- Run / experiment:
- up to 75 minutes
- Value:
- 4.3
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- percent corneal swelling
- Run / experiment:
- up to 240 minutes
- Value:
- 11.2
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: slight indication of irritation
- Irritation parameter:
- cornea opacity score
- Value:
- 3.3
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of irritation
- Irritation parameter:
- fluorescein retention score
- Value:
- 3
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- positive indication of irritation
- Interpretation of results:
- Category 1 (irreversible effects on the eye) based on GHS criteria
- Conclusions:
- Based on this in vitro eye irritation assay in isolated chicken eyes with diammonium tetrachlorozincate(2-), the test item is irritant, UN GHS Classification: Category 1.
- Executive summary:
An in vitro eye irritation study of the test item was performed in isolated chicken’s eyes. The irritation effects of the test item were evaluated according to the OECD No. 438 guideline (09 October 2017).
After the zero reference measurements, the eye was held in horizontal position and 30 mg powdered test itemwas applied onto the centre of the cornea in such a way that the entire surface of the cornea was covered. After 10 seconds, the surface was rinsed with physiological saline. Positive control eyes were treated with 30 mg powdered Imidazole. The negative control eye was treated with 30 µL ofphysiological saline(0.9% (w/v) NaCl solution). In the study, three test item treated eyes, three positive control treated eyes and one negative control treated eye were examined.
The results from all eyes used in the study met the quality control standards. The negative control and positive control results were within the historical control data range in the experiment. Thus, the experiment was considered to be valid.
Slight corneal swelling was observed during the four-hour observation period on test item treated eyes. Severe cornea opacity change (severity 2 on one eye and severity 4 on two eyes) was observed on three eyes. Severe fluorescein retention change (severity 3) was noted on all three eyes.Test item was stuck on all cornea surfaces after the post-treatment rinse. The cornea surfaces were cleared at 30 minutes after the post-treatment rinse.
Based on this in vitro eye irritation assay in isolated chicken eyes with diammonium tetrachlorozincate(2-),the test item is irritant,UN GHS Classification: Category 1.
Reference
The mean values of the treated eyes for maximum corneal thickness change, corneal opacity and fluorescein retention are given below. The conclusion on eye irritancy was based on the OECD guideline quantitative assessments.
Observation |
Value |
ICE Class |
Mean maximum corneal swelling at up to 75 min |
4.3 % |
I |
Mean maximum corneal swelling at up to 240 min |
11.2 % |
II |
Mean maximum corneal opacity |
3.33 |
IV |
Mean fluorescein retention |
3.00 |
IV |
Other Observations |
Test item was stuck on all cornea surfaces after the post-treatment rinse. The cornea surfaces (3/3) were cleared at 30 minutes after the post-treatment rinse. |
|
Overall ICE Class |
1xII 2xIV |
Based on this in vitro eye irritation in the isolated chicken eyes test with diammonium tetrachlorozincate(2-),the test item is irritant,UN GHS Classification: Category 1.
Positive Control
Observation |
Value |
ICE Class |
Mean maximum corneal swelling at up to 75 min |
9.6 % |
II |
Mean maximum corneal swelling at up to 240 min |
24.1 % |
II |
Mean maximum corneal opacity |
4.00 |
IV |
Mean fluorescein retention |
3.00 |
IV |
Other Observations |
Imidazole was stuck on all cornea surfaces after the post-treatment rinse. The cornea surfaces (3/3) were not cleared at 240 minutes after the |
|
Overall ICE Class |
1xIII 2xIV |
The positive controlImidazole was classified as severely irritating, UN GHS Classification: Category 1.
NEGATIVE Control
Observation |
Value |
ICE Class |
Mean maximum corneal swelling at up to 75 min |
0.0 % |
I |
Mean maximum corneal swelling at up to 240 min |
0.0 % |
I |
Mean maximum corneal opacity |
0.00 |
I |
Mean fluorescein retention |
0.00 |
I |
Other Observations |
None |
|
Overall ICE Class |
3xI |
The negative control Physiological saline was classified as non-irritating, UN GHS Classification: No Category.
Table:Assessment of the general IN VITRO eye irritancy and regulatory GHS classification.
UN GHS Classification |
Combinations of the three ICE Classes |
No Category |
3×I 2×I, 1×II |
No prediction can be made |
Other combinations |
Category 1
|
3×IV 2×IV, 1×III 2×IV, 1×II* 2×IV, 1×I* Corneal opacity ≥ 3 at 30 min (in at least 2 eyes) Corneal opacity = 4 at any time point (in at least 2 eyes) Severe loosening of epithelium (in at least 1 eye) |
Remark:*:combinations of categories less likely to occur
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irreversible damage)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Justification for classification or non-classification
Based upon OECD 431/439 in vitro skin irritation/corrosion data, diammonium tetrachlorozincate does not require classification as a skin irritant
Based upon OECD 438 in vitro eye irritation data, diammonium tetrachlorozincate does require classification as an severe eye irritant.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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