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EC number: 203-940-1 | CAS number: 112-15-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From August 18, 2020 to December 17, 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Version / remarks:
- 2012-10-02
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source of test material:
Production lot
- Lot/batch number of test material: P230220120
- Expiration date of the lot/batch:
2023-04-29
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
Cool, well ventilated place
- Stability under storage conditions: Stable
- Stability under test conditions: Stable - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 2.0, 3.0, 4.5, 6.8 and 10.1 mg/L
- Sampling method: 5 mL of test samples from each replicate were drawn and mixed together for each group at 0 (fresh medium) and 48 hours (exhausted medium) of formulations prepared on days 0, 10 and 18.
- Sample storage conditions before analysis: If required, at -20 ± 5 ºC - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Dilution from stock solution of test substance prepared with test medium
- Controls: Test medium alone
- Chemical name of vehicle: None used
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Age at study initiation: 1st instar daphnids, less than 24 hours old
- Feeding during test : Yes
- Food type: unicellular alga, Pseudokirchneriella subcapitata
- Amount: 0.1 - 0.2 mg carbon/daphnid/day
- Frequency: Daily
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 179.2 - 196.0 mg/L as CaCO3
- Test temperature:
- 20.0 - 20.9 °C
- pH:
- 7.59 and 7.96
- Dissolved oxygen:
- 7.39 - 9.23 mg/L
- Nominal and measured concentrations:
- Nominal: 0.0, 2.0, 3.0, 4.5, 6.8 and 10.1 mg/L
Measured: 0.0 (< LOD), 2.1, 3.2, 4.7, 7.3 and 10.8 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 600 mL capacity glass beaker
- Material, size, headspace, fill volume: Glass, 600 mL capacity, 120 mL fill volume
- Renewal rate of test solution (frequency/flow rate): Semi-static, medium changed every 48 hours
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water prepared as described by OECD test giuidelines
- Culture medium different from test medium: No
- Intervals of water quality measurement: Weekly
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light / 8 hours dark
- Light intensity: 1300 - 1380 Lux
EFFECT PARAMETERS MEASURED
The adults were observed for mortality, if any, on a daily basis.
The numbers of live and dead offspring produced were recorded daily and removed from the glass beakers.
On termintion of the study absolute length (body length excluding the anal spine) of parent daphnids was measured using a stereo zoom microscope following anaesthesia.
Temperature, pH, dissolved oxygen and hardness, were measured at least once a week, in fresh and old media.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5
- Range finding study: Yes
- Test concentrations: Range-finding study - 0.0, 0.1, 1.0, 5.0, 10.0 and 50.0 mg/L
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 10.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 10.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 10.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality: Total number living offspring produced at the end of the test per parent daphnia at the start of the test excluding from the analysis parental accidental and/or inadvertent mortality {TG 211}
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 10.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality: Total number living offspring produced at the end of the test per parent daphnia at the start of the test excluding from the analysis parental accidental and/or inadvertent mortality {TG 211}
- Details on results:
- - Behavioural abnormalities:
No treatment related effects
- Observations on body length: No treatment related effects
- Other biological observations: Time to release of first brood - 8 days in control and all treatment groups
- Mortality of control: None
- Other adverse effects control: None
- Immobilisation of control: None
- Abnormal responses: None observed
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No - Reported statistics and error estimates:
- Brood number and body length were assessed using Bartlett’s Test and Analysis of Variance followed by Dunnett’s procedure.
As no effects of treatment were observed, the EC10, EC20 and EC50, values for reproductive output could not be calculated. - Validity criteria fulfilled:
- yes
- Conclusions:
- 2-(2-ethoxyethoxy)ethyl acetate has no toxic effect up to 10.1 mg/L on daphnia magna and this is deemed the No Observed Effect Concentration (NOEC).
- Executive summary:
The reproductive output of Daphnia magna exposed to different concentrations of 2 -(2 -ethoxyethoxy)ethyl acetate over a period of 21 days has been examined using methods described by OECD TG 211.
The substance has no toxic effect at concentrations up to 10.1 mg/L and this is deemed the No Observed Effect Concentration (NOEC).
Reference
Live and Dead Offspring and Adult Mortality
Nominal Concentration (mg/L) |
Number of Daphnids/ Group |
Total Number of Adult Mortality |
Total Number of Dead Offspring |
Total Number of Live Offspring |
Live Offspring
|
% Inhibition in Offspring |
|
Mean |
CV |
||||||
0.0 (Control) |
10 |
0 |
0 |
803 |
80.3 |
1.2 |
- |
2.0 |
10 |
0 |
0 |
802 |
80.2 |
1.1 |
0.12 |
3.0 |
10 |
0 |
0 |
805 |
80.5 |
1.8 |
-0.25 |
4.5 |
10 |
0 |
0 |
802 |
80.2 |
2.3 |
0.12 |
6.8 |
10 |
0 |
0 |
804 |
80.4 |
1.9 |
-0.12 |
10.1 |
10 |
0 |
0 |
804 |
80.4 |
4.4 |
-0.12 |
Key: CV = Coefficient variation, - = Not applicable
Daphnia Length
Nominal Concentration (mg/L) |
Mean Value Daphnia Length in Millimeter (mm) |
SD |
0.0 (Control |
3.38 |
0.18 |
2.0 |
3.34 |
0.20 |
3.0 |
3.34 |
0.16 |
4.5 |
3.33 |
0.16 |
6.8 |
3.35 |
0.21 |
10.1 |
3.40 |
0.17 |
Key: SD = Standard deviation
Description of key information
The substance has no toxic effect at concentrations up to 10.1 mg/Land this is deemed the No Observed Effect Concentration (NOEC).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- 10.1 mg/L
Additional information
A single study is available examining the reproductive output of Daphnia magna exposed to different concentrations of 2 -(2 -ethoxyethoxy)ethyl acetate over a period of 21 days. Methods used were as described by OECD TG 211. The substance has no toxic effect at concentrations up to 10.1 mg/Land this is deemed the No Observed Effect Concentration (NOEC).
A further, unreliable, study has been published investigating the long-term effects to Ceriodaphnia dubia (Devillers et al., 2003). The study was performed according to AFNOR NF T 90-376. After an exposure of 7 days an EC10 of 0.125 mg/L resulted. This study has not been considered for classification purposes due to the presence of a more recent study using test methods and organisms as specified by Regulation (EC) 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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