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EC number: 203-940-1 | CAS number: 112-15-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- Activated sludge O2 consumption
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23 Jul - 20 Sep 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Data based on the result from a ready biodegradability study. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2008).
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Circular on Test Methods of New Chemical Substances (Japan), Biodegradation test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- other: mixture of activated sludge from STP with freshwater from rivers and lakes, marine water and marine sediment
- Details on inoculum:
- - Source of inoculum/activated sludge (location, sampling depth): location: 10 sites (4 sewage plants, 3 rivers, 1 lake and 2 bays) around Japan, sampling depth: return sludge in sewage plants, surface water and surface soil of foreshore
- Laboratory culture: Thirty minutes after stopping of aeration, discard about one third of the whole volume of supernatant and add an equal volume of a solution (pH adapted to 7.0 with NaOH) containing 0.1% each of glucose, peptone and potassium orthophosphate, to the settled material and re-commence aeration. This procedure was repeated once per day.
- Temperature: 25 ± 2 °C
- Storage conditions: observed appearance and general condition of sludge, determined sediments, pH, temperature and dissolved oxygen and observed with a light microscope, whether it is normal.
- Storage length: started to use: 16 Jul 1991
- Preparation of inoculum for exposure: Activated sludge from STP mixed with
- Concentration of sludge: 4600 mg/L
- Water filtered: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 28 d
- Test temperature:
- 25 ± 1 °C
- pH:
- 5.6 - 7.2
- Nominal and measured concentrations:
- nominal: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: closed oxygen consumption measuring machine with 300 mL culture bottle (coulometer)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 1
EFFECT PARAMETERS MEASURED: biodegradation of the test substance based on BOD, TOC and GC-analysis - Reference substance (positive control):
- no
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Result from ready biodegradability test
- Validity criteria fulfilled:
- yes
- Conclusions:
- Toxicity to microorganisms was investigated in a biodegradation study according to OECD 301C. No inhibition of biodegradation was observed after 28 days and the tested concentration of 100 mg/L can be used as NOEC value for the toxicity to activated sludge microorganisms.
- Executive summary:
The toxicity of 2-(2-ethoxyethoxy)ethyl acetate (CAS 112-15-2) to microorganisms was investigated in a biodegradation study according to OECD 301C. No inhibition of biodegradation was observed after 28 days and the tested concentration of 100 mg/L can be used as NOEC value for the toxicity to activated sludge microorganisms.
Reference
The initial inoculum concentration of 100 mg/L was degraded to an extend of 101% based on BOD, 95% based on TOC and 100% based on GC analysis. The substance is readily biodegradable according to the OECD criteria. Thus, the test substance is not considered to be inhibitory to activated sludge microorganisms.
Description of key information
NOEC (28 d) ≥ 100 mg/L for activated sludge microorganisms (OECD 301C)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
The toxicity of 2-(2-ethoxyethoxy)ethyl acetate (CAS 112-15-2) to microorganisms was investigated in a biodegradation study according to OECD 301C under GLP conditions (Ministry of Economy, Trade and Industry, Japan, 1991). The study was performed with a mixture of activated sludge from STP with freshwater from rivers and lakes, marine water and marine sediment. No inhibition of biodegradation was observed after 28 days. The test concentration from a biodegradation study which indicated that the test substance is readily biodegradable can be used to derive a NOEC for this endpoint as stated in the ECHA Guidance R.7b (ECHA, 2012). Therefore, the test item concentration of 100 mg/L can be used as NOEC value for the toxicity to activated sludge microorganisms. Thus a NOEC (28 d) of ≥ 100 mg/L resulted.
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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