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EC number: 203-940-1 | CAS number: 112-15-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Description of key information
Oral: LD50 = 3930 mg/kg bw
Inhalation: LC0 = ca. 1 mg/L
Dermal: LD50 = > 2000 mg/kg bw
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Principles of method if other than guideline:
- Single oral administration by stomach tube in male rats.
- GLP compliance:
- no
- Test type:
- standard acute method
- Species:
- rat
- Strain:
- other: albino Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 90 - 120 g
- Diet: Purina chows, supplemented by fresh vegetables
- Water: ad libitum - Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: maximal concentration 500 mg/mL
MAXIMUM DOSE VOLUME APPLIED: Individual dose levels were not reported for any of the substances tested including the test substance considered herein. According to the authors, the highest dose level which could safely be hold at one time by the animal was just over 10% of the animal’s bodyweight, corresponding to approx. 50 g/kg bw, when a substance was administered as a 50% solution. This is equivalent to a dose volume of 100 mL/kg bw.
Furthermore, it was reported that in most cases 10 animals were dosed to determine the toxicity of a particular dosage and enough dosages were given to include those at which no mortality occurred and those at which all tested animals died.
The test substance was administered as a 50% solution and based on the information above and the resulting LD50 value, it appears unlikely that the test substance could have been administered at a dose volume > 100 mL/kg bw.
It is therefore assumed that the maximum dose volume applied was 100 mL/kg bw corresponding to a maximum dose level of 50000 mg/kg bw. - Doses:
- Not specified (presumably up to 50000 mg/kg bw)
- No. of animals per sex per dose:
- ca. 10
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs - Statistics:
- The data were calculated by the method of probits, described by Bliss C.I. (1935. The calculation of the dosage-mortality curve. Ann. Appl. Biol. 22:134-167).
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 11 000 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 10 400 - 11 590
- Remarks on result:
- other: slope: 21.21
- Mortality:
- Mortality data were summarised for all substances tested. Therefore, no substance specific data were reported.
According to the authors, most deaths occurred within the first 48 h post-dose, but all deaths within 14 days post-administration were taken into account for calculation of LD50 values. In particular for ethers, death was delayed about a week. - Clinical signs:
- other: The test substance was tested along with other glycol ethers. For this group, narcosis was observed but in most cases only at the LD50 or above.
- Gross pathology:
- Gross pathology findings were summarised for all substances tested. According to the authors, all doses caused some degree of irritation of the digestive tract. The primary target organ was the kidney; blood in urine and free blood beneath the capsule were seen at the highest dosages. The liver was less affected, but orange or reddish bile was often observed.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- CLP: not classified
DSD: not classified - Executive summary:
Median lethal dose (LD50) following administration of a single oral dose to the rat was in excess of 2000 mg/kg body weight.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Quality of whole database:
- The available information comprises adequate, reliable (Klimisch score 2) and consistent studies, and is thus sufficient to fulfil the standard information requirements set out in Annex VII, 8.5, of Regulation (EC) No 1907/2006.
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Principles of method if other than guideline:
- 8 h exposure to saturated vapour at room temperature to rats
- GLP compliance:
- no
- Test type:
- standard acute method
- Species:
- rat
- Strain:
- not specified
- Sex:
- not specified
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- not specified
- Vehicle:
- not specified
- Analytical verification of test atmosphere concentrations:
- no
- Duration of exposure:
- 8 h
- Concentrations:
- saturated vapour (ca. 1 mg/L)
- No. of animals per sex per dose:
- no data
- Control animals:
- not specified
- Details on study design:
- - Necropsy of survivors performed: yes, 2 rats were killed for examination two weeks after exposure.
- Other examinations performed: heart, liver, kidney, spleen and lung were observed. - Sex:
- not specified
- Dose descriptor:
- LC0
- Effect level:
- ca. 1 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 8 h
- Remarks on result:
- other: saturated vapour concentration based on molecular weight = 176.21 g/mol and vapour pressure = 13.19 Pa at 20°C
- Mortality:
- None of the test animals died.
- Gross pathology:
- The tissues from the two rats were completely normal.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- CLP: not classified
DSD: not classified
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Quality of whole database:
- The available information comprises adequate, reliable (Klimisch score 2) and consistent studies, and is thus sufficient to fulfil the standard information requirements set out in Annex VII, 8.5, of Regulation (EC) No 1907/2006.
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Principles of method if other than guideline:
- Single dermal application of the test substance to guinea pigs.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- guinea pig
- Strain:
- not specified
- Sex:
- male/female
- Type of coverage:
- not specified
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Type of wrap if used: cotton poultice - Doses:
- 5, 10, 15, 20 and 30 mL/kg
- No. of animals per sex per dose:
- 10 (each in total for 5 and 30 mL/kg)
4 (in total for 20 mL/kg)
8 (in total for 15 mL/kg)
12 (in total for 10 mL/kg) - Control animals:
- not required
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- >= 30 000 mg/kg bw
- Based on:
- test mat.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- CLP: not classified
DSD: not classified - Executive summary:
Median lethal dose (LD50) following dermal administration to the guinea pig was in excess of 2000 mg/kg body weight.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Quality of whole database:
- The available information comprises studies which each alone are regarded insufficient for assessment (Klimisch score 2-4). However, the information from these independent sources is consistent and provides sufficient weight of evidence for hazard assessment leading to an endpoint conclusion in accordance with Annex XI, 1.2, of Regulation (EC) No 1907/2006. Therefore, the available information as a whole is sufficient to fulfil the standard information requirements set out in Annex VII, 8.5, of Regulation (EC) No 1907/2006.
Additional information
Acute oral toxicity
Two acute oral toxicity studies with 2-(2-ethoxyethoxy)ethyl acetate in rats and guinea pigs are available (Smyth, 1941). 2 -(2-ethoxyethoxy)ethyl acetate was administered as single oral dose by stomach tube in male rats and guinea pigs.
In rats and guinea pigs, groups of 10 animals received 2-(2-ethoxyethoxy)ethyl acetate as 50% dispersion in water. The observation period following administration was 14 days. The data were calculated by the method of probits. The precision is indicated by the range of 95% probability. The resulting LD50 value of the study in male rats was evaluated to be 11000 mg/kg bw and for guinea pigs 3930 mg/kg bw. Thus, the oral LD50 of 2-(2-ethoxyethoxy)ethyl acetate (CAS 112-15-2) is considered to be greater than 3930 mg/kg bw.
Acute inhalation toxicity
An acute inhalation toxicity study with 2-(2-ethoxyethoxy)ethyl acetate was performed in rats and guinea pigs (Smyth, 1939). Animals were exposed for 8 h to saturated vapour of the test material at room temperature. No mortality occurred and the observed tissues from two rats were normal. One guinea pig showed minor reversible changes in the kidney and another guinea pig showed slight evidence of lung irritation (no further information). Thus, the LD0 value for rats and guinea pigs was ca. 1 mg/L air.
Acute dermal toxicity
The acute dermal toxicity of 2-(2-ethoxyethoxy)ethyl acetate was evaluated by a single dermal application to guinea pigs (Smyth, 1940). Groups of 4 to 12 animals were treated with 5, 10, 15, 20 and 30 mL/kg of the undiluted test substance. The resulting LD50 value was greater than 30000 mg/kg bw.
In addition, a study report was cited in the SIDS Initial Assessment Report For SIAM 21 (OECD SIDS). 2-(1-methylethoxy)ethyl acetate was tested in rabbits and a LD50 value of 15000 mg/kg bw was evaluated.
Thus, the results of the two studies showed no effects at or exceeding the limit dose of 2000 mg/kg bw. Therefore, the dermal LD50 is considered to be greater than 2000 mg/kg bw.
Conclusion for acute toxicity
In summary, studies from 2-(2-ethoxyethoxy)ethyl acetate (CAS 112-15-2) investigating the acute oral toxicity in rats and guinea pigs resulted in oral LD50 values of 11000 and 3930 mg/kg bw, respectively, and thus greater than the limit dose value of 2000 mg/kg bw.
For acute inhalation toxicity, a study with 2-(2-ethoxyethoxy)ethyl acetate (CAS 112-15-2) in rats and guinea pigs are available. From these studies, a LC0 value of ca. 1 mg/L (saturated vapour concentration) was obtained.
Acute dermal toxicity studies conducted with 2-(2-ethoxyethoxy)ethyl acetate (CAS 112-15-2) showed no effects at or exceeding the limit dose of 2000 mg/kg bw, resulting in an dermal LD50 values of 15000-30000 mg/kg bw..
Thus, the available data indicate a very low level of acute toxicity and thus no hazard for acute oral, inhalative and dermal toxicity was identified.
Justification for classification or non-classification
The available data on acute, dermal and inhalation toxicity of 2-(2-ethoxyethoxy)ethyl acetate (CAS 112-15-2) do not meet the criteria for classification according to Regulation (EC) 1272/2008 or Directive 67/548/EEC, and the data are therefore conclusive but not sufficient for classification.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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