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Diss Factsheets
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EC number: 203-466-5 | CAS number: 107-13-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Acrylonitrile
- EC Number:
- 203-466-5
- EC Name:
- Acrylonitrile
- Cas Number:
- 107-13-1
- Molecular formula:
- C3H3N
- IUPAC Name:
- prop-2-enenitrile
Constituent 1
Method
- Target gene:
- No details
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium, other: TA100, TA 98, TA 1535, TA 1537 and TA 1538
- Species / strain / cell type:
- E. coli, other: WP2 uvr A and WP2 uvrA/pKM101
- Metabolic activation:
- with and without
- Metabolic activation system:
- SD rat (7 week old, male) liver, induced with PCB (KC-500)
- Test concentrations with justification for top dose:
- -S9 mix / +S9 mix ; 0, 100, 250, 500, 1,000, 2,500, 5,000 μg/plate
- Vehicle / solvent:
- Vehicle(s)/solvent(s) used: Water
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamid(-S9 mix: S. typhimurium TA100, TA98, E.Coli WP2 uvrA and WP2 uvrA/pKM101)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- (-S9 mix : S. typhimurium TA1535)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- (-S9 mix : S. typhimurium TA1537)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (+S9 mix: S. typhimurium TA 100, TA 98 and TA 1535 and E. coli WP2 uvr A and WP2 uvrA/pKM101
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- (+S9 mix : S. typhimurium TA 1537, 1538)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
Preincubation : 20 minutes
Temperature : 37 ˚C
Exposure duration: 2 days
NUMBER OF PLATES : Solvent / vehicle controls :3, Positive controls : 2, Test substance : 2
NUMBER OF TEST REPEAT : 2
- Evaluation criteria:
- A test substance is considered to be mutagenic when a dose-related increase in revertant colony count is observed and the number of revertant colonies per plate with the test substance is more than twice that of the negative control and when a reproducibility of test result is observed. Additionally a test substance for which the results do not meet the above criteria is considered non-mutagenic in this system.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium, other: S. typhimurium TA 100, TA 98, TA 1537, TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not specified
- Species / strain:
- E. coli, other: WP2 uvr A and WP2 uvrA/pKM101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not specified
- Remarks on result:
- other: strain/cell type: S. typhimurium TA 1535
Any other information on results incl. tables
Results of the bacterial reversion test of acrylonitrile [direct method: -S9]
AF-2: 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamid ENNG : N-ethyl-N-nitro-N-nitrosoguanidine 9AAC : 9-aminoacridine 2NF : 2-nitrofluorene
Table2. Results of the bacterial reversion test of acrylonitrile [direct method: +S9]
2AA : 2-aminoanthracene B(a)P : benzo(a)pyrene |
Applicant's summary and conclusion
- Conclusions:
- Acrylonitrile was found to be mutagenic under the conditions of this study [positive: weak positive in TA1535 (+S9)] .
- Executive summary:
The mutagenicity of acrylonitrile was examined using preincubation method in S. typhimurium TA 100, TA 98, TA 1535, TA 1537, TA 1538 and E. coli WP2uvr A and WP2 uvrA/pKM101, at concentrations up to 5000 μg/plate with or without a metabolic activation system. In this study, slight positive mutagenic responses were obtained in TA 1535 with metabolic activation. Therefore, acrylonitrile is considered to be mutagenic.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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