2-piperazin-1-ylethylamine

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not applicable

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: 2e: Meets generally accepted scientific standards, well-documented and acceptable for assessment

Data source

Reference

Reference 1

Reference Type:

publication

Title:

No information

Author:

Smyth, H.F. et al

Year:

1962

Bibliographic source:

Am Ind Hyg Assoc J, vol 23 ; p. 95

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1956

Report date:

1956

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Essentially followed the method of Draize et al.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-piperazin-1-ylethylamine

EC Number:

205-411-0

EC Name:

2-piperazin-1-ylethylamine

Cas Number:

140-31-8

Molecular formula:

C6H15N3

IUPAC Name:

2-piperazin-1-ylethanamine

Details on test material:

AEP reported to contain 2.5% diethylenetriamine.

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male

Details on test animals or test system and environmental conditions:

Groups of four male New Zealand white rabbits, 3 to 5 months of age and averaging 2.5 kg were used.

The rabbits were procured locally and maintained on Rockland rabbit ration.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

not specified

Details on dermal exposure:

The fur is removed and the test material applied beneath an impervious plastic film

Duration of exposure:

The test material is in contact for 24 hours after which the plastic film is removed and the rabbits are held for a 14-day observation period.

Doses:

0.625 or 1.25 ml of test material/kg

No. of animals per sex per dose:

4 males/dose

Control animals:

not specified

Details on study design:

Rabbits were immobilized during the 24-hour skin contact period. Thereafter, the "Vinylite" sheeting used to retain the dose in contact with the clipped skin of the trunk was removed and the animals were caged for the remainder of the 14-day observation period.

Statistics:

Thompson's method of calculating the LD50 was used.

Results and discussion

Preliminary study:

Not applicable

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

866 mg/kg bw

Mortality:

One of three male rabbits dosed with 0.625 ml/kg died and two of three male rabbits dosed with 1.25 ml/kg died. All deaths occurred within 2 days.

Clinical signs:

other: No additional information available.

Gross pathology:

Congestion of the lungs, mottling of the livers and pitting or speckling of the kidney surfaces was observed (not stated whether this was in the survivors or animals that died).

Other findings:

No additional information available.

Any other information on results incl. tables

These covered applications produced necrosis of the skin, congestion of the lungs, mottling of the livers and pitting or speckling of the kidney surf aces.

Aminopropyl rnorpholine has a comparable value of 1.2 m l /kg. and diethylene trimine 1.1 ml/kg.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Remarks:

Migrated information

Conclusions:

The 24 hour dermal LD50 was 0.88 ml/kg (866 mg/kg).

Executive summary:

By skin penetration on rabbits the LD50 is 0.88 (0.34 to 2.3) ml/kg (866 mg/kg). This is to be expected because of the necrotic action of the mixture on rabbit skin which destroys the skin barrier entirely.