Reaction mass of 2-hydroxy-1,3-propanediyl bismethacrylate and 101525-90-0

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999-12-06 - 1999-12-10

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

GLP compliance:

no

Specific details on test material used for the study:

Gylcerol: 1,3 dimethacrylate
Batch No: 790703330
Lot: No. 32079289
Reactive ester content: 96.9 %
Diester content: 90.5 %
Triester content: 1.5 %
Inhibitor: 202 ppm HQME
Methacrylic acid: 0.2 %

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 10.7, 18.8. 32.7, 57.1 and 100 mg/l

Vehicle:

no

Details on test solutions:

- The individual test concentrations were prepared by dilution of stock solution

Test organisms (species):

Poecilia reticulata

Details on test organisms:

Test organism: Poecilia reticulata (Guppy)
- 7 fish per test convcentration (length of 1-3 cm)
Breeding: Parental and young fish population was held in two 250 I glass aquaria
Illumination: 14 h a day
Temperature: 23 ± 2 °C
Medium: Continuously aerated and filtered tap water from Schlieren. The bottom of the aquaria was covered with gravel (51.5 em). Every month, one
third of the total volume of the aquaria was replaced by fresh tap water.
Feed: Sera micropan, Sera vipan (Sera apanristic GmbH, D-52525 Heinsberg, Germany) daily.
The fish were last fed 24 h before the start of the definitive test.
Measurements: pH, conductivity, oxygen concentration and hardness of water were determined once a week.
Temperature was controlled by a thermostatic heating element.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

17.7 (minimum) - 31.9 (maximum) °F total hardness

Test temperature:

21 ± 1 °C

pH:

8.3 - 8.7

Dissolved oxygen:

0.76 - 8.4 mg oxygen/l

Nominal and measured concentrations:

10.7, 18.8. 32.7, 57.1 and 100 mg/l (nominal)
Reactive test concentrations after 24 h of incubation: 94-98 %

Details on test conditions:

Medium: Continuously aerated and filtered tap water from Schlieren. The bottom of the aquaria was covered with gravel (51.5 em). Every month, one
third of the total volume of the aquaria was replaced by fresh tap water.
Feed: Sera micropan, Sera vipan (Sera apanristic GmbH, D-52525 Heinsberg, Germany) daily.
The fish were last fed 24 h before the start of the definitive test.
Measurements: pH, conductivity, oxygen concentration and hardness of water were determined once a week.
Temperature was controlled by a thermostatic heating element.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

43.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

57.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

32.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

 

Nominal concentration (mg/l)	Cumulative mortality observed at different points in time after start of exposure
	2h	24h	48h	72h	96h
control 0	0	0	0	0	0
10.7	0	0	0	0	0
18.7	0	0	0	0	0
32.7	0	0	0	0	0
57.1	0	0	3	4	7
100	0	7	a)	a)	-a)

 

Validity criteria fulfilled:

yes

Remarks:

During 7 days before the definitive test, mortality in the batch of which fish were used for the test was less than 5 %.

Conclusions:

LC50 fish (96h): 43.2 mg/l

Executive summary:

The acute lethal toxicity (LC50) of GLYCEROL-1 ,3-DIMETHACRYLATE to Poecilia reticulata (Guppy) was investigated under semi-static exposure conditions over a period of 96 h acc. OECD 203 (fish acte toxicity test adopted 1992).

The test medium was replaced every 24 h. 7 fish per concentration were exposed to the test substance.

Nominal concentrations of GLYCEROL-1,3-DIMETHACRYLATE as selected after a preceding limit test were 10.7, 18.7, 32.7, 57.1 and 100 mg/1, respectively.

The individual test concentrations were prepared by dilution of a stock solution. HPLC analyses of the test substance concentration were conducted at the beginning and at the end of every 24 h cycle.

The analyses revealed that the test substance concentrations were satisfactorily maintained over the whole incubation time of 96 h.

In the definitive test all fish died at the nominal concentrations of 100 mg/1 after 24 h of exposure. After 96h all fish were dead at the nominal concentrations of 57.1 mg/1. No mortality was observed at all other test concentrations and in the control after 96 h of exposure. Other toxic effects than mortality, e.g. loss of coordination, hypoactivity and swimming on the back, were not observe

LC50 (96h): 43.2 mg/1 (based on nominal conentrations)

NOEC (96h): 32.7 mg/1.

LC100 (96h): 57.1 mg/1.

Description of key information

The acute lethal toxicity (LC50) of GLYCEROL-1 ,3-DIMETHACRYLATE to Poecilia reticulata (Guppy) was investigated under semi-static exposure conditions over a period of 96 h acc. OECD 203

Result: LC50 (96h): 43.2 mg/1 (based on nominal concentrations).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

43.2 mg/L

Additional information

Aquatic toxicity of Glycerol 1,3-dimethacrylate is based on narcosis which correlates with log Kow. Log Kow values of substances containing ether groups are known to differ from measured values which means for Glycerol 1,3 dimethacrylate that the calculated log Kow value is lower than measured value. (see Schüürmann G (1990) QSAR Analysis of the acute toxicity of oxyethylated surfactants,

Chemosphere, Vol.21, Nos.4-5, oo 467-47 8 , 1990, Printed in Creat Britain).

The measured log Kow is 2.05. QSAR predictions with OECD toolbox version 3.3 are performed KOWWIN 1.68 which calculates a log Kow of 1.16.

The QSAR prediction of acute fish toxicity was performed to demonstrate on the one hand that the predicted LC50 of 133 mg/l is higher than the measured but primarily that the experimental LC50 fish of 43.2 mg/l correlates with the measured log Kow. Based on this correlation in a further step QSAR predictions for daphnia magna and algae were performed (see 6.1.3 and 6.1.5) and the corrected EC50 values were graphically read for the measured logKow.