Registration Dossier
Registration Dossier
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EC number: 820-225-5 | CAS number: 101747-77-7
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
A study with the source substance Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259 -15 -8) was performed to assess the effect of the test material on the growth of the green alga Scenedesmus subspicatus. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No. 201, “Alga Growth Inhibition Test” and was performed under GLP conditions. The quality of the study was assessed with Klimisch 2. Exposure of Scenedesmus subspicatus to the test material gave an EbL50 (72h) (based on biomass) value of 240 mg/L loading rate WAF and an ErL50 (72hr) (based on growth rate) value of 410 mg/L loading rate WAF. The No Observed Effect Loading rate was 220 mg/L loading rate WAF.
A study with the source substance Phosphorodithioic acid, mixed O,O-bis(1,3 -diemtylbutyl and iso Pr) esters, zinc salts (CAS 84605 -29 -8) was performed to assess the effect of the test material on the growth of the green alga Scenedesmus subspicatus. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No. 201, “Alga Growth Inhibition Test” and was performed under GLP conditions.The quality of the study was assessed with Klimisch 2. Exposure of Scenedesmus subspicatus to the test material gave an EbL50 (72h) (based on biomass) value of 21 mg/L loading rate WAF and an ErL50 (72hr) (based on growth rate) value of 24 mg/L loading rate WAF. The No Observed Effect Loading rate was 10 mg/L loading rate WAF.
The values are different from each other. But this is in range with the category approach, where nevertheless the members of the categories may have different values.
For the CSA the lower value is chosen as worst-case value.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 24 mg/L
- EC10 or NOEC for freshwater algae:
- 10 mg/L
Additional information
Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259 -15 -8)
Introduction:
A study was performed to assess the effect of the source substance Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259 -15 -8) on the growth of the green alga Scenedesmus subspicatus. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No. 201, “Alga Growth Inhibition Test”.
Methods:
Following a preliminary range finding test, the alga were exposed to Water Accommodated Fractions of test material over a range of nominal loading rates of 10, 32, 220, 320, and 1,000 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 degrees C. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group using a Coulter Multisizer Particle Counter.
Results:
Exposure of Scenedesmus subspicatus to the test material gave an EbL50 (72h) value of 240 mg/L loading rate WAF and an ErL50 (0-72 hr) value of 410 mg/L loading rate WAF. The No Observed Effect Loading rate was 220 mg/L loading rate WAF.
Phosphorodithioic acid, mixed O,O-bis(1,3 -diemtylbutyl and iso Pr) esters, zinc salts (CAS 84605 -29 -8)
Introduction:
A study was performed to assess the effect of the test material on the growth of the green alga Scenedesmus subspicatus. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No. 201, “Alga Growth Inhibition Test”.
Methods:
Following a preliminary range finding test, the alga were exposed to Water Accommodated Fractions of test material over a range of nominal loading rates of 10, 20, 40, 80, and 160 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 °C. Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group using a Coulter Multisizer Particle Counter.
Results:
Exposure of Scenedesmus subspicatus to the test material gave an EbL50 (72h) (based on biomass) value of 21 mg/L loading rate WAF and an ErL50 (72hr) (based on growth rate) value of 24 mg/L loading rate WAF. The No Observed Effect Loading rate was 10 mg/L loading rate WAF.
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