Registration Dossier
Registration Dossier
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EC number: 820-225-5 | CAS number: 101747-77-7
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Based on the different approaches of the two tests (respiration vs. growth inhibition) both studies are seen as key studies:
Source substance Phosphorodithioic acid, mixed O,O-bis(1,3 -dimethylbutyl and iso-Pr) esters, zinc salts (CAS 84605 -29 -8):
A study was performed under GLP to assess the effect of the test material on the respiration of activated sewage sludge (Klimisch 2). The method followed that described in the OECD Guidelines for Testing of Chemicals (1984), No. 209 "Activated Sludge, Respiration Inhibition Test".
The effect of the test material on the respiration of activated sewage sludge micro-organisms using standard guidelines gave an EC50 of >10,000 mg/L.
Source substance Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259 -15 -8):
A study was performed under GLP to assess the effect of the test material on the growth inhibition of Pseudomonas putida (Klimisch 1). The method followed that described in the DIN EN ISO 10712 Pseudomonas putida growth inhibition test.
The effect of the test material on growth inhibition of Pseudomonas putida leads to an EC50 of 380 mg/L and an EC0 of 200 mg/L.
For CSA the lower value is choosen, also with regard, that growth inhibition is an earlier stage than respiration inhibition.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 380 mg/L
- EC10 or NOEC for microorganisms:
- 200 mg/L
Additional information
Phosphorodithioic acid, mixed O,O-bis(1,3 -dimethylbutyl and iso-Pr) esters, zinc salts (CAS 84605 -29 -8):
Introduction:
A study was performed under GLP to assess the effect of the test material on the respiration of activated sewage sludge. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984), No. 209 "Activated Sludge, Respiration Inhibition Test".
Methods:
Activated sewage sludge was exposed to directly added test material at concentrations of 1, 10, 100, 1000 and 10000 ppm, a duplicate control group, and an assessment of the sensitivity of the inoculum used in the test to a reference toxicant 3,5-dichlorophenol. The test duration was a 3 hour exposure period at 20 °C to the test material followed by measurement of oxygen consumption.
Results:
The effect of the test material on the respiration of activated sewage sludge gave an EC50 of >10,000 mg/L. The reference material gave an EC50 value of 18.5 mg/L. The activated sludge respiration test passed the quality control criteria for an acceptable test. The two control replicates produced oxygen consumption rates within the required 15% of each other.
Source substance Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259 -15 -8):
A study was performed under GLP to assess the effect of the test material on the growth inhibition of Pseudomonas putida. The method followed that described in theDIN EN ISO 10712 Pseudomonas putida growth inhibition test.
The effect of the test material on growth inhibition of Pseudomonas putida leads to an EC50 of 380 mg/L and an EC0 of 200 mg/L.
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