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EC number: 221-070-0 | CAS number: 2997-92-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Water solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30.07.-08.08.2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Qualifier:
- according to guideline
- Guideline:
- EU Method A.6 (Water Solubility)
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- flask method
- Water solubility:
- > 168.05 - < 188.5 g/L
- Temp.:
- 20 °C
- pH:
- > 5.57 - < 6.55
- Remarks on result:
- other: based on individual measurements
- Key result
- Water solubility:
- ca. 176.2 g/L
- Temp.:
- 20 °C
- Remarks on result:
- other: mean
- Conclusions:
- Interpretation of results (migrated information): very soluble (> 10000 mg/L)
The water solubility of 2,2'-azobis[2-methylpropionamidine] dihydrochloride is determined by the flask method to be 176.2 g/L. - Executive summary:
The solubility of 2,2'-azobis(2 -amidinopropane)dihydrochloride in water was determined from the measured concentrations of test item in the filtrated and diluted test solutions.
In a preliminary study, solubility of the test item in water was estimated as approx. 200 g/L. The flask method was used for the determination of the solubility of the test item in water. In the main study, 50 - 85 g test item were weighed into each of six individual vessels, water was added, and the vessels were shaken at 20.0 ± 0.5 °C.
In several steps, additional volumes of water were added, with total volumes ranging from 100 - 150 mL, because of the high viscosity of the mixtures. The additions resulted in loading rates of 500 - 727 g/L. On days 2, 5, and 6, vessel 1 was taken from the shaking apparatus and storred at 20.0 ± 0.5 °C for equilibration. Samples were taken, filtrated, diluted and analysed using HPLC as shaking of the test vessels was continued.
No differences above 15 % in the concentration of the solution could be determined between the sampling dates 5 and 6, supporting the hypothesis, that the maximum concentration of the test item had been reached. Therefore, on day 6, vessels 2 - 6 were sampled and analysed. Dependency of the solubility on amount of the test item (nominal load) was not perceived.
At the plateau, the concentration of 2,2'-azobis(2 -amidinopropane)dihydrochloride in water had reached 176.2 ± 7.6 g/L at 20.0 ± 0.5 °C.
Reference
Measurements for Temperature Dependency of Water Solubility
The data which was acquired for temperature dependency of water solubility is presented in the following table:
Flask | Temperature in °C | Test item conc. (diluted sample) in mg/L | Dilution factor | Test item Conc. (original sample) in g/L | Difference to previous sample % / °C |
1 | 20 | 204.5 | 1000 | 204.5 | |
2 | 5 | 168.7 | 1000 | 168.7 | 1.2 |
Because the difference between the measured concentrations of the two flasks was < 3 % / °C (1.2 % / °C), the main study was performed at 20 °C only.
Measurements during Equilibration
the data which was acquired during equilibration time (flask 1) is presented in the following table:
Day | Temperature in °C | pH* | Test item conc. (diluted sample) in mg/L | Dilution factor | Test item conc. (original sample) in g/L | Difference to previous sample % |
1 | 20.3 | 6 -7 | 206.61 | 1000 | 206.61 | -- |
2 | 20.1 | 6-7 | 187.43 | 1000 | 187.43 | -9.3 |
5 | 20.5 | 6 -7 | 191.04 | 1000 | 191.04 | 1.9 |
6 | 20.3 | 6 -7 | 171.47 | 1000 | 171.47 | -10.2 |
After the analysis of the sample of day 10, the plateau was considered as reached.
Measurements at Plateau, Result
The concentrations of test item were determined from the measured values following the equation (y=0.1082*x-0.0478). The following table gives the values which were measured at the plateau. A standard deviation of 7.6 g/L was calculated giving a relative standard deviation of 4.3 %.
Flask | 1 | 2 | 3 | 4 | 5 | 6 |
pH | 5.57 | 6.55 | 6.14 | 6.28 | 6.33 | 6.13 |
Measured values (Area in mAU*min) | 18.5023 | 17.4944 | 19.1901 | 19.3993 | 18.3957 | 20.4564 |
Concentration test item in mg/L (diluted sample) | 171.45 | 168.05 | 178.50 | 179.85 | 170.90 | 188.50 |
Dilution factor | 1000 | 1000 | 1000 | 1000 | 1000 | 1000 |
Concentration test item in g/L (original sample) | 171.45 | 168.05 | 178.50 | 179.85 | 170.90 | 188.50 |
Temperature was measured with 19.5 - 20.3 °C.
Mean at the plateau is 176.2 ± 7.6 g/L
Description of key information
The water solubility of 2,2'-azobis[2-methylpropionamidine] dihydrochloride is determined by the flask method to be 176.2 g/L.
Key value for chemical safety assessment
- Water solubility:
- 176.2 g/L
- at the temperature of:
- 20 °C
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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