Captan

Toxicity to terrestrial plants

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20 September 2011 to 11 Oktober 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 227 (Terrestrial Plant Test: Vegetative Vigour Test)

Version / remarks:

2006

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

- Physical state: Light brown granules
- Analytical purity: 80.8 % (w/w)
- Batch number: 91131140
- Expiry date: 2013-06-03
- Storage conditions: 2011-08-18 to 2011-08-25: Room temperature, protected from light, in original container. Since 2011-08-25: 6 ± 2 °C, protected from light, in original container.

Analytical monitoring:

yes

Vehicle:

yes

Remarks:

tap water

Details on preparation and application of test substrate:

The test solution was prepared in tap water by directly weighing of the respective test item amounts

Species:

Avena sativa

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

oats, ivorySaaten Union GmbH, Eisenstrafie 12, D-30916 Isernhager HB

Species:

Allium cepa

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

onion, ExhibitionHildSamen GmbH, Postfach 1161, D-71666 Marbach

Species:

Beta vulgaris

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

sugar beet, Beretta EPDKWS Saat AG, Grimsehlstr. 31. Postfach 1463, D-37555 Einbeck

Species:

Brassica napus

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

rape, DiggerKWS Saat AG, Grimsehlstr. 31. Postfach 1463, D-37555 Einbeck

Species:

Daucus carota

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

carrot, Merida f1Hild Samen GmbH, Postfach 1161, D-71666 Marbach

Species:

Glycine max (G. soja)

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

soybean, Petrina PZOPZO Saat GmbH, Oberiimpurg 2, D-74523 SchwSbisch Hall

Test type:

vegetative vigour test

Study type:

laboratory study

Substrate type:

natural soil

Remarks:

with addition of 30% quatz sand

Limit test:

yes

Total exposure duration:

21 d

Test temperature:

14 - 22° C

Moisture:

43 - 96% relative humidty

Details on test conditions:

Bottom watering including fertilizer

Nominal and measured concentrations:

#1 nominal: 1619 mg/L; measured 1569 mg/L
#2 nominal: 16191 mg/L; measured 15223 mg/L

Key result

Species:

Avena sativa

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Height

Remarks on result:

other: No phytotoxic effects

Key result

Species:

Allium cepa

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Height

Remarks on result:

other: No phytotoxic effects

Key result

Species:

Beta vulgaris

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Height

Remarks on result:

other: No phytotoxic effects

Key result

Species:

Brassica napus

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Height

Remarks on result:

other: No phytotoxic effects

Key result

Species:

Daucus carota

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Height

Remarks on result:

other: No phytotoxic effects

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Height

Remarks on result:

other: No phytotoxic effects

Key result

Species:

Avena sativa

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Fresh Weight

Remarks on result:

other: No phytotoxic effects

Key result

Species:

Allium cepa

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Fresh Weight

Remarks on result:

other: No phytotoxic effects

Key result

Species:

Beta vulgaris

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Fresh Weight

Remarks on result:

other: No phytotoxic effects

Key result

Species:

Brassica napus

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Fresh Weight

Remarks on result:

other: No phytotoxic effects

Key result

Species:

Daucus carota

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Fresh Weight

Remarks on result:

other: No phytotoxic effects

Key result

Species:

Glycine max (G. soja)

Duration:

21 d

Effect conc.:

1.92 kg/ha

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

phytotoxicity

Remarks:

Inhibition of Shoot Fresh Weight

Remarks on result:

other: No phytotoxic effects

Visual Phytotoxicity: The plants were observed on day 7, 14 and 21 for visual phytotoxic effects and number of dead plants. No phytotoxic effects were observed for all tested plant species at the limit application rate of 2.4 kg formulation/ha.

The percent Inhibition of shoot height and shoot fresh weight is given in Table 6.3.3-2  to Table 6.3.3-3.

 

Table 6.3.3-2 Inhibition of Shoot Height

Application rate [kg/ha]	Shoot height [cm]
	Oats	Inhib. [%]	Onion	Inhib. [%]	Sugar Beet	Inhib. [%]	Rape	Inhib. [%]	Carrot	Inhib. [%]	Soybean	Inhib. [%]
Control	60.9	-	37.8	-	16.5	-	23.2	-	29.8	-	43.1	-
2.4	60,7	0	39.8	-5	17.3	-5	22.4	3	30.7	-3	40.8	5

inhib. = Inhibition negative values = promoted grovrth

 

Table 6.3.3-3 Inhibition of Shoot Fresh Weight

Application rate [kg/ha]	Shoot Fresh Weight [g]
	Oats	Inhib. [%]	Onion	Inhib. [%]	Sugar Beet	Inhib. [%]	Rape	Inhib. [%]	Carrot	Inhib. [%]	Soybean	Inhib. [%]
Control	19.03	-	15.38	-	27.73	-	36.99	-	20.87	-	27.99	-
2.4	19.28	-1	14.64	5	32.04	-16	34.85	6	22.56	-8	27.93	-1

  inhib. = Inhibition negative values = promoted grovrth

Validity criteria fulfilled:

yes

Remarks:

OECD 227 (2006): The results of the control for all plant species met the required validity criteria

Conclusions:

No phytotoxic effects were observed for ail tested plant species at the limit application rate of 2.4 kg Captan/ha, corresponding to 1.92 kg a.i./ha (nominal). The test item did not cause inhibitory effects ≥ 10% on shoot height and shoot fresh weight (biomass) to all plant species at the application rate of 2.4 kg Captan/ha, corresponding to 1.92 kg a.i./ha (nominal).

Executive summary:

The phytotoxicity of captan to six terrestrial plant species was established in 21-day toxicity tests. Test plants comprised two monocotyledons (oats, Gramineae; onion, Liliaceae) and four dicotyledons (sugar beet, Chenopodiaceae; rape, Brassicaceae; carrot, umbelliferae; soybean, Leguminosae). The test was conducted with the limit application rate of 2.4 kg formulation/ha, corresponding to 1.92 kg captan/ha. The test item was applied at test start to plant foliage which had reached the 2-4 true leaf stage. Potential toxic effects of the test item were assessed on day 7, 14 and 21 by visual observations (phytotoxic effects and number of dead plants) and on day 21 additionally by shoot height and shoot fresh weight determination. No phytotoxic effects were observed for all tested plant at the limit application rate of 2.4 kg formulation/ha.

The test Item did not cause inhibitory effects ≥ 10 % on shoot height and shoot fresh weight (biomass) to all plant species at the application rate of 2.4 kg formulation/ha (Table 6.3.3-1).

 

Table 6.3.3-1 Inhibition of Shoot Height and Shoot Fresh Weight in Comparison to the Control

	Inhibition [%]
Species	Shoot height	Shoot fresh weight
Oats	0	-1
Onion	-5	5
Sugar beet	-5	-16
Rape	3	6
Carrot	-3	-8
Soybean	5	-1

negative values = promoted growth

Description of key information

Toxicity to terrestrial plants: Vegetative Vigour Test (6 non-target plant species, 21 days post application), application rate: 2.4 kg formulation/ha ≡ 1.92 a.i./ha: no phytotoxic effects were observed, OECD (2007)

Additional information

Captan is used as a fungicide on a wide range of crop groups and does not cause crop phytotoxicity. No phytotoxic effects were observed in a test with 6 different crop plants (including both monocotyledon and dicotyledon plant species) at dose rates of 1.92 kg captan/ha indicating a low risk to non-target organisms.