Acetic acid, oxo-, sodium salt, reaction products with ethylenediamine and hydroxybenzenesulfonic acid monosodium salt, iron sodium salts

Short-term toxicity to fish

Description of key information

Fe(3K)EDDHSA (EC 462-490-6): Oncorhynchus mykiss, OECD 203: LC50(96h) > 100 mg/L 
Fe(Na)EDDHA (CAS 84539-55-9): Danio rerio, OECD 203: LC50(96h) > 120 mg/L 
Rainbow trout, Carp, Catfish and Bluegill, no guideline followed: LC50(96h) > 100 mg/L 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

The acute toxicity to freshwater or marine fish was not investigated so far for the substance Fe(3Na)EDDHSA (CAS 84539-54-8). To fulfil this relevant endpoint for the intended registration under REACH, a read-across from the closely related substances Fe(3K)EDDHSA (EC 462 -490 -6) and Fe(Na)EDDHA (CAS 84539-55-9) was performed.

The nearest neighbour Fe(3K)EDDHSA (EC 462-490-6) was tested in an experiment with rainbow trout (Onchorhynchus mykiss), exposed over a 96 hour. The study was conducted according to the principles of OECD Guideline 203 (L´Hardon, 2000). The test organism is a freshwater fish representative of a wide variety of natural habitats, and can therefore be considered as an important non-target organism in freshwater ecosystems. The test concentration to be used in the definitive test was determined by a preliminary range-finding test. Based on this range-finding study, the substance was tested a limit test with a singe test concentration of 100 mg/L.The feeding was discontinued 24 h prior to the start of the definitive test. At the start of the test 7 fish were placed in the test vessel at random, giving a loading rate of 1 g fish/L. The experiment was performed at a 13 - 17 °C with a photoperiod of 16 h light and 8 h darkness.The control group was maintained under identical test conditions but not exposed to the test material. No abnormalities as well as no dead fish were detected in this group. Also in the test group, neither mortality nor any toxic signs were observed, the LC50(96h) value is greater than tested concentration of 100 mg/L.

Two experimental reports are available for Fe(Na)EDDHA (CAS 84539-55-9) concerning short-term toxicity to freshwater fish. The zebrafish (Danio rerio) was used as test organism for an exposure period of 96 hour exposure period in an experiment conducted according to the principles of OECD Guideline 203 and EU Method C.1 (Salinas, 2010). A limit test was performed with an analytically confirmed test concentration of 120 mg/L. Therefore, no statistical analysis was performed. Since neither mortality nor toxic signs were observed, the LC50(96h) value is greater than tested concentration of 120 mg/L based on nominal concentrations. Based on the mean of analytically determined concentration, a value of greater or equal to 122 mg/L is reported. Since the analytically determined concentrations of the test substance in the test solutions were within +/- 20 % of the nominal concentrations, the effect concentration is expressed relative to the nominal concentration. The corresponding NOEC was therefore determined to be greater than or equal to 120 mg/L.

Furthermore, the acute toxic effects of Fe(Na)EDDHA to Rainbow trout, Carp, Catfish and Bluegill were determined by Sachssee and Barthe (1977). The fish were tested in glass tanks of 12 and 15 litre capacity. Testing was performed in reconstituted water prepared of deionised water. Various concentrations of Fe(Na)EDDHA were prepared by adding the test material dissolved in aqua dist. (Stock solution). Solubility of active ingr. in water: well miscible. At the appropriate amount ensuring that the total volume in each vessel remained the same. In this acute toxicity test with Rainbow trout, Carp, Catfish and Bluegill the 96 h-LC50 was greater than 100 mg/L. These results are based on nominal concentrations. Fe(Na)EDDHA is practically devoid of acute toxicity to Trout, Carp, Catfish and Bluegill when tested and evaluated according to the procedure of Bathe et al.