Acetic acid, oxo-, sodium salt, reaction products with ethylenediamine and hydroxybenzenesulfonic acid monosodium salt, iron sodium salts

Long-term toxicity to aquatic invertebrates

Description of key information

Fe(Na)EDDHMA (CAS 84539-53-7), Daphnia magna, OECD 211: NOEC(21d): 320 mg/L (reproduction) NOEC(21d) >= 100 mg/L (parental mortality).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

320 mg/L

Additional information

Chronic toxicity effects towards aquatic invertebrates of Fe(3Na)EDDHSA (CAS 84539-54-8) were not investigated experimentally. Read across from the closely related substance Fe(Na)EDDHMA (CAS 84539-53 -7) is performed to fulfil this relevant endpoint under REACH (for further details, please refer to the separate read-across statement).

Fe(Na)EDDHMA were used for the investigation of survival and reproductive Performance in Daphnia magna during 21 days of exposure according to OECD Guideline 211 (Bogers, 1995). The nominal concentrations tested were 10, 32, 100, 320 and 1000 mg/L. The test solutions were renewed three times a week. 10 neonate daphnids for the test concentrations and 20 animals for the control group were investigated. At the start of the test and every workday, the condition of the parental daphnids was recorded and during the reproduction phase the number of living offspring, immobile young and the presence of unhatched eggs were recorded. During the study duplicate samples of 10 mL were taken from freshly prepared solutions on days 0, 7, 14 and 19, from 72-hour old solutions on day 3 and from 48-hour old solutions on day 21. Samples were stored in a deepfreeze until possible analysis. Analysis of the samples showed that the method of preparation of test media provided repeatable recoveries of test substance concentration. Measured concentrations were all in agreement with nominal, except for those measured in the samples taken at 1000 mg/L (85 % - 89 %) during the 2 -day period after the last renewal. Further, the measured concentrations were stable during the periods between renewals of the test solutions. No mortality or other visible effects were recorded for the parental organisms at any of the test item concentrations tested.During the total exposure period of 21 days, no significant (greater than 10%) immobilisation (including mortality) of new-born young was observed at any of the test concentrations. In conclusion, the test substance had no effect on the survival of Daphnia magna at concentrations up to and including 1000 mg/L in this reproduction study. The test item affected the reproductive capacity at concentrations of 320 and 1000 mg/L. A biologically relevant effect on reproductive capacity was noted at 1000 mg/L.