3-aminomethyl-3,5,5-trimethylcyclohexylamine

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Remarks:

bacterial growth of Pseudomonas putida

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Principles of method if other than guideline:

Method: other: Bringmann and Kühn (1977), Z. Wasser Abwasser Forsch. 10,
87-98

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

- Stock solution: 5.00 g test substance/l (were made up with bidistilled water ); gave pH = 11.5, which was neutralized with 10 % hydrochloric acid.
-Test concentrations ( 500 mg/L; 1000 mg/L ; 2000 mg/L and 4000 mg/L) were prepared by diluting the stock solution with bidestilled water.

Test organisms (species):

Pseudomonas putida

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

18 h

Remarks on exposure duration:

+/- 1h

Test temperature:

25 +/- 2 degree C

pH:

7.1 (Test 1); 7.0 (Test 2)

Nominal and measured concentrations:

Nominal: control / 500 / 1,000 / 2,000 / 4,000 mg/l

Details on test conditions:

- Number of replicates: 5 growth controls 4 controls 3 replicates of 4 concentrations all performed twice
- Test vessel: 250 ml Erlenmeyer flasks, sterile, capped with cellulose
- Duration of test: 18 +/- 1 hours
- Quantification: photometric determination of turbidity at 436 nm, graphical evaluation

Reference substance (positive control):

not specified

Key result

Duration:

18 h

Dose descriptor:

EC10

Effect conc.:

1 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: extinction/absorption

EC10 = 1200 mg/l (Test 1)
1040 mg/l (Test 2);
mean = 1120 mg/l

Validity criteria fulfilled:

not specified

Conclusions:

The EC10 (18 hours) was determined as 1120 mg/L indicating that the test substance does not pose a significant hazard to aquatic microorganism

Executive summary:

In a bacterial toxicity test using Pseudomonas putida (Hüls AG, 1996b) an 18 h-EC10 of 1,120 mg/l was obtained. Four concentrations ranging from 500 to 4000 mg/L were tested. The study was assessed as "reliable with restrictions".

Description of key information

In a bacterial toxicity test using Pseudomonas putida (Hüls AG, 1996b) an 18 h-EC10 of 1120 mg/l was obtained.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

1 120 mg/L

Additional information

18 h-EC10 value of 1120 mg/l