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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 204-424-9 | CAS number: 120-78-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- not specified
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- not specified
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - activated sludge: 6.0 g/l dry mass per liter
- inoculum: 50 ml sludge/ l test volume - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20.5 - 22.4°C
- pH:
- 7.5 - 8.3
- Dissolved oxygen:
- 2.0-8.4 mg/l
- Nominal and measured concentrations:
- Nominal concentrations:
- test substance: 100, 1000, and 10000 mg/l
- reference substance: 1 and 20 mg/l - Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- On 3.5-dichlorophenol (20 mg/l) an inhibition of 78% was obtained.
- Conclusions:
- Toxicity of MBTS to activated sludge was evaluated and no toxic effect was observed up to the highest nominal concentration tested (10000 mg/l) during 3 hours exposure at a pH 7.5 - 8.3 and temperature of 20.5 - 22.4 °C.
- Executive summary:
Toxicity of MBTS to activated sludge was evaluated by Bayer AG (1988) according to the ISO 8192 -1986/B and no toxic effect was observed up to the highest nominal concentration tested (10000 mg/l) during 3 hours exposure at a pH 7.5 - 8.3 and temperature of 20.5 - 22.4 °C.
- Endpoint:
- activated sludge nitrification inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- No guideline available at the moment of testing. Inhibition of nitrification process.
- GLP compliance:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Remarks on exposure duration:
- 2-4 h test period
- Test temperature:
- 25°C
- pH:
- 7.6 - 7.8
- Dissolved oxygen:
- > 1 mg/l
- Salinity:
- 50 - 80 mg/l ammonia
0.003 mol/l NaHCO3 - Details on test conditions:
- pre-cleaned activated sludge in particle-free communal waste water
- Dose descriptor:
- other: EC75
- Effect conc.:
- 38 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks on result:
- other: reported test duration: 2-4 hours
- Conclusions:
- To the nitrification inhibition by 2,2'-disulfanediylbis(1,3-benzothiazole) an EC75 of 38 mg/L during a 2 -4 h incubation period was obtained.
- Executive summary:
To the nitrification inhibition by 2,2'-disulfanediylbis(1,3-benzothiazole) an EC75 of 38 mg/L during a 2 -4 h incubation period was obtained. (Tomlinson, Boon, and Trotman, 1966).
Referenceopen allclose all
In sewage treatment plant, large amount of test substance might be present in both dissolved and undissolved form. To represent a more realistic situation, the tested concentrations are much higher than the water solubility of the test substance (i.e. 88 mg/l at pH 5 and 49mg/l at pH 9). Therefore the reported concentrations represent the combined effect of dissolved and undissolved test substance on the activity of microorganism in the sewage treatment plant.
the reported test duration was 2 - 4 hours
Description of key information
Toxicity of MBTS to activated sludge was evaluated by Bayer AG (1988) according to the ISO 8192 -1986/B and no toxic effect was observed up to the highest nominal concentration tested (10000 mg/l) during 3 hours exposure at a pH 7.5 - 8.3 and temperature of 20.5 - 22.4 °C. To the nitrification inhibition by 2,2'-disulfanediylbis(1,3-benzothiazole) an EC75 of 38 mg/L during a 2 -4 h incubation period was obtained (Tomlinson, Boon, and Trotman, 1966).
Key value for chemical safety assessment
Additional information
In sewage treatment plant, large amount of test substance might be present in both dissolved and undissolved form. To represent a more realistic situation, the tested concentrations in Bayer AG study (1988) are much higher than the water solubility of the test substance (i.e. 88 mg/l at pH 5 and 49mg/l at pH 9). Therefore the reported concentrations represent the combined effect of dissolved and undissolved test substance on the activity of microorganism in the sewage treatment plant.
From the different inocula tested, the nitrification inhibition on non-adapted sludge was most sensitive and should be taken for further risk assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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