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Diss Factsheets
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EC number: 200-662-2 | CAS number: 67-64-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study meets basic scientific principles; open epicutaneous exposure to the volatile test substance is expected to reduce the actual dermal uptake; study sufficiently documented, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- A new protocol and criteria for quantitative determination of sensitization potencies of chemicals by guinea pig maximization test
- Author:
- Nakamura A, Momma J, Sekiguchi H, Noda T, Yamano T, Kaniwa M-A, Kojima S, Tsuda M, Kurokawa Y
- Year:
- 1 994
- Bibliographic source:
- Contact Dermatitis 31: 72-85
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Guinea pig maximization test (Magnusson and Kligman, 1969) with modified scoring scale: determination of background sensitization rate as acetone is used as vehicle for other substances
- GLP compliance:
- not specified
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- Existing study, performed before LLNA was available as OECD guideline
Test material
- Reference substance name:
- Acetone
- EC Number:
- 200-662-2
- EC Name:
- Acetone
- Cas Number:
- 67-64-1
- Molecular formula:
- C3H6O
- IUPAC Name:
- propan-2-one
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- female
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal and epicutaneous
- Vehicle:
- unchanged (no vehicle)
- Concentration / amount:
- 100 %
Challengeopen allclose all
- Route:
- epicutaneous, open
- Vehicle:
- unchanged (no vehicle)
- Concentration / amount:
- 100 %
- No. of animals per dose:
- 10
- Details on study design:
- A. INDUCTION EXPOSURE
Induction was performed by intradermal injection and topical application of 100 % acetone according to the protocol of Magnusson and Kligman (no further details available)
B. CHALLENGE EXPOSURE
Challenge was performed 21 days after the initial intradermal injection by topical open application of 0.1 ml 100 % acetone to the shaved area of the flank
C. EVALUATION
48 hrs after the challenge application skin reactions were avaluated according to a modified scale for scoring:
erythema formation: grades from 0 to 4
edema formation: grades from 0 to 3
possible total maximum: 7
sensitization rate: number of animals with positve reaction/number of tested animals
mean response: [sum from all animals of (erythema score + edema score)] : numer of tested animals - Positive control substance(s):
- yes
- Remarks:
- other substances tested, e.g. 2-mercaptobenzothiazole
Results and discussion
- Positive control results:
- Positve responses were found with 2-mercaptobenzothiazole with a sensitization rate of 5/5 and mean responses uo to 5.0 (depending on induction and challlenge concentrations)
In vivo (non-LLNA)
Results
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 100 %
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 48.0. Group: test group. Dose level: 100 %. No with. + reactions: 0.0. Total no. in groups: 10.0.
Any other information on results incl. tables
Sensitization rate 0/10; mean response 0
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information
- Conclusions:
- No indications of a sensitizing potential of acetone were found in a guinea pig maximization test.
- Executive summary:
Acetone, used as vehicle for other test substances, was tested in a guinea pig maximization test (protocol of Magnusson and Kligman with modified scale for scoring of skin responses) with a group of 10 female Hartley guinea pigs. Induction was performed by intradermal injection and topical application of 100 % acetone. 21 days later the animals were challenged by topical application of 100 µl acetone on the shaved flank. No positive skin response was found in any of the animals.
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