A mixture of cis- and trans-cyclohexadec-8-en-1-one

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1986-10-16 to 1987-01-27

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

The test process was not reported in details.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

1981

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Not reported

Duration of test (contact time):

28 d

Initial conc.:

30 mg/L

Based on:

test mat.

Initial conc.:

15 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

The test was conducted in 2 L glass flasks closed with screw caps from which carbon dioxide traps were suspended. Each flask was filled with 1 litre of medium inoculated with 10 mL of the supernatant of a settled sewage sludge. The medium was freed from carbon dioxide.
The test was run in duplicate for the medium blanks and the toxicity controls containing sodium acetate, and in quadruplicate for the two concentrations of test item. Incubation took place at 20°C an a rotary shaker in the Bark.

Reference substance:

acetic acid, sodium salt

Parameter:

% degradation (CO2 evolution)

Value:

72

Sampling time:

28 d

Remarks on result:

other: test concentration: 15 mg/L

Key result

Parameter:

% degradation (CO2 evolution)

Value:

74

Sampling time:

28 d

Remarks on result:

other: test concentration: 30 mg/L

Results with reference substance:

Not reported

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test item was determined to be readily biodegradable.

Executive summary:

The study on the ready biodegradability of the item was conducted according to OECD 301B and GLP. Test was performed using so-called sturm method. Two concentrations (15 mg test item/L and 30 mg test item/L) were tested and sodium acetate was used as reference substance in the process control and toxicity control. The production of CO2 was measured and used as the parameter to calculate the percentage of the biodegradation. Under the test condition, 72-74% of the carbon from the test compound was released as carbon dioxide (leaving about 30% for growth of micro-organisms), the test item was considered as readily biodegradable. 

Description of key information

A study on the ready biodegradability of the item is available. The study was conducted according to OECD 301B (so-called sturm method) and GLP. Two concentrations (15 mg test item/L and 30 mg test item/L) were tested and sodium acetate was used as reference substance in the process control and toxicity control. The production of CO2 was measured and used as the parameter to calculate the percentage of the biodegradation. Under the test condition, 72-74% of the carbon from the test compound was released as carbon dioxide (leaving about 30% for growth of micro-organisms), the test item was considered as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater