A mixture of cis- and trans-cyclohexadec-8-en-1-one

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000-06-14 to 2000-07-05

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

1998

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

GC-analysis

Details on sampling:

Sampling schedule of chemical analysis:
Stock solution: on day 0, 2, 5, 7, 9, 12, 14, 16, 19;
Control: on day 0, 7, 16 in the new media; on day 2, 97, 19 in the old media;
Test concentrations: 0, 7, 16 in the new media; on day 2, 97, 19 in the old media;

Vehicle:

no

Details on test solutions:

A stock solution was prepared to give the desired series of test concentrations. To achieve this 1.0 mg of the test substance was added to 1 litre of dilution water and treated for 60 seconds at 8000 rpm with an Ultra turrax and stirred for 1 hour on a magnetic stirrer.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water floh
- Strain/clone: Daphnia magna STRAUS,
- Source: Strain of Bundesgesundheitsamt Berlin

ACCLIMATION
- Acclimation period: A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility under constant temperature conditions
- Acclimation conditions (same as test or not): yes
- Type and amount of food:Scenedestnus subspicatus CHODAT
- Feeding frequency: ad libitum
- Health during acclimation (any mortality observed): no

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

258.8-283.8 mg/L CaCO3

Test temperature:

18-22 °C

pH:

Control: 7.8-8.3;
0.023 mg/L: 7.8-8.3

Dissolved oxygen:

Control: 8.2-9.6 mg O2/L
0.023 mg/L: 8.2-10.3 mg O2/L

Salinity:

n.a.

Conductivity:

n.a.

Nominal and measured concentrations:

Nominal concentrations: 0 (control)-0.0007-0.0023-0.007-0.023 mg/L;
Mean measured concentrations: < 0.0005 (control) -0.00025*-0.0016-0.0045-0.0141 mg/L (*expected below the LOQ (0.0005 mg/L), half the quantitation limit was used)

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beaker
- Type (delete if not applicable): open
- Material, size, fill volume: 150 mL glass beaker, 100 mL
- Renewal rate of test solution (frequency/flow rate): every 24 hours
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Medium: M4 medium
- Intervals of water quality measurement: once per week

OTHER TEST CONDITIONS
- Photoperiod: 16/8 light/ darkness
- Light intensity: =< 88 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.3

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 0.014 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 0.014 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

See Below

Immobilisation/mortality rate and mean reproduction rate of parent Daphnia:

Nominal concentration [mg/L]	Mean measured concentration [mg/L]	Immobilisation/mortality rate of parent Daphnia		Mean production rate of parent Daphnia
		absolute	%	absolute	%
Control	< 0.0005	0	0	151.7 [n=10]	100
0.0007	---	0	0	165.4 [n=10]	109.0
0.0023	0.0016	0	0	161.0 [n=10]	106.1
0.007	0.0045	0	0	159.4 [n=10]	105.1
0.023	0.0141	1*	10*	172.0 [n=10]	113.4

*) This mortality rate is not regarded to be statistically different from the control as, according to OECD 211, a mortality rate lower than 20% (in the control vessels) does not invalidate the test results.

Validity criteria fulfilled:

yes

Conclusions:

The values of 21d-NOEC (reproduction and mortality) were determined to be above 0.0141 mg/L based on the mean measured concentrations.

Executive summary:

The study on the biological effect of the test item on the reproduction of parthenogenetic Daphnia magna STRAUS was conducted according to OECD 211 and GLP. The test was carried out under semi-static conditions using 4 concentrations. The test concentrations were 0 (control) - 0.0007 - 0.0023 - 0.007 - 0.023 mg/L. The concentrations were measured via GC-method in the new and old media. The test concentration were not remained within +/-20% of the nominal concentrations. Therefore, the biological effects were expressed on the basis of the mean measured concentrations. The values of 21d-NOEC (reproduction and mortality) were determined to be above 0.0141 mg/L.

Description of key information

A study on the biological effect of the test item on the reproduction of parthenogenetic Daphnia magna STRAUS is available. The test was conducted according to OECD 211 and GLP under semi-static conditions. Four test concentrations and a control were tested. They were 0 (control) - 0.0007 - 0.0023 - 0.007 - 0.023 mg/L with a sparation factor of 3.3. The concentrations were measured via GC-method in the new and old media. The test concentration were not remained within +/-20% of the nominal concentrations. Therefore, the biological effects were expressed on the basis of the mean measured concentrations. The values of 21d-NOEC (reproduction and mortality) were determined to be above 0.0141 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

NOEC

Effect concentration:

>= 0.014 mg/L