Methyl hydrogen 2-nitroterephthalate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994 - 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl hydrogen 2-nitroterephthalate

EC Number:

252-360-5

EC Name:

Methyl hydrogen 2-nitroterephthalate

Cas Number:

35092-89-8

Molecular formula:

C9H7NO6

IUPAC Name:

4-(methoxycarbonyl)-3-nitrobenzoic acid

Details on test material:

purity: 94.0 +-0.5 %

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Substance / water mixture was homogenized using an Ultrr-Turrax and an Ultrasonic bath

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish, Brachydanio rerio
- Strain: Hamilton-Buchanan
- Source: Hoechst AG, Corporate Toxicology
- Age at study initiation (mean and range, SD):part 1: hatching: 1994-07-02 start: 1994-12-23
part 2: hatching: 1994-08-18 start: 1994-12-27
- Length at study initiation: part 1: 2.9 - 3.2 cm; mean 3.1 cm
part 2: 2.8 - 3.1 cm; mean 2.9 cm

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions : 22+-1 °C; oxygen content: > 80% of the saturation value
- Type and amount of food: Tetra Min; Tetra Werke, Melle (FRG)
- Feeding frequency: twice daily ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

total hardness: 2.1 - 2.5 mmol Ca2+ plus Mg2+ /L

Test temperature:

21.5 - 22.2 °C

pH:

7.0 - 8.2

Dissolved oxygen:

7.8 - 9.4 mg O2/L

Nominal and measured concentrations:

nominal (mg/L) measured (mg/L)
22 18.1
50 40.7
100 78.3

Details on test conditions:

TEST SYSTEM
- Test vessel: glass vessel ( 30 cm x 22 cm x 24 cm)
- Type: open
- fill volume: 10 L
- Aeration: no
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water, composition according to ISO/DIS 7346/1
Dilution water was aerated until oxygen saturation
- Intervals of water quality measurement: weekly

OTHER TEST CONDITIONS
- Photoperiod: illumination from above from 6:00 am to 6:00 pm
- Light intensity: 700 lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

other: lethality: 2 from 7

Effect conc.:

40.7 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

18.1 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

78.3 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: hypoactivity, dark colored integument, swimming posture tail-heavy, projecting opercula, swimming at the water surface

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the the results of this test, the subtsance has to be considered as harmful for fish.

Executive summary:

Nitro-MMT was tested for acute toxicity to fish. The study was performed in two parts; part 1 with 0 mg/Land 50 mg/L (40.7 mg/L measured), and part 2 with 0, 22, and 100 mg/L (18.1 and 78.3 mg/L measured). Symptoms and lethality were observed at 40.7 (2 from 7) and 78.3 mg/L (7 from 7). The 18.1 mg/L group exhibited reversible symptoms but no lethality.

Based on this a LC50 can be predicted in the range from 40.7 - 78.3 mg/L