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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed study according to a standard guideline, with GLP

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
Deviations:
yes
Remarks:
reference substance was tested in concentrations of 30 mg/l and 90 mg/L
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl hydrogen 2-nitroterephthalate
EC Number:
252-360-5
EC Name:
Methyl hydrogen 2-nitroterephthalate
Cas Number:
35092-89-8
Molecular formula:
C9H7NO6
IUPAC Name:
4-(methoxycarbonyl)-3-nitrobenzoic acid
Details on test material:
purity: 94.0 +- 0.5 %

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida Migula, DSM 50026

Study design

Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
16 h

Test conditions

Test temperature:
25 - 30 °C
Reference substance (positive control):
yes
Remarks:
2,4-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
16.3 h
Dose descriptor:
other: - 67 % inhibition
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
16.3 h
Dose descriptor:
other: - 43% inhibition
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
The test item supports the growth of Pseudomonas putida
Results with reference substance (positive control):
2,4-Dichlorophenol after 16.3 hours_
30 mg/L : 26,2 % inhibition
90 mg/L: 97.6 % inhibition

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test item is not toxic for Pseudomonas putida. It supports its growth. EC50 > 1000 mg/L
Executive summary:

Nitro-MMT was tested for toxicity against Pseudomonas putida. Up to the highest concentration of 1000 mg/L no inhibition occured.

Therefore, EC50 is > 1000 mg/L and NOEC is 1000mg/L.