2-isocyanatoethyl methacrylate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-Guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Remarks:

Ministerium für Arbeit, Integration und Soziales des Landes Nordrhein-Westfalen, Germany

Analytical monitoring:

yes

Details on sampling:

- Concentrations: The degradation products were analysed in samples of all freshly prepared test solutions and control solutions at test start, and from pooled replicates per test concentration and controls at test end. The samples were analysed immediately after sampling.
- Sampling method: The samples were taken by the staff of the analytical laboratory and transferred to the analytical laboratory for measurement without delay or further storage.
- Sample storage conditions before analysis: Duplicate samples were frozen and stored at 20°C.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A solution was prepared by dissolving 100.2 mg of the test item MOI in 1000 mL of copper-reduced dilution water and stirring for 2 h at 300 rpm. This solution formed the highest test concentration. The remaining test concentration solutions were prepared by a 50% dilution series with copper-reduced dilution water of the highest test concentration solution. The test solutions were freshly prepared immediately before the start of the test.
- Controls: The control test media was copper-reduced dilution water taken directly from the pipe immediately before the start of the test.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Waterflea
- Strain:
- Source: Juvenile daphnids were originally obtained from the German Federal Environmental Agency, Institute for Water, Soil, and Air Hygiene. Specimens used in the test were bred in the laboratory at Fraunhofer IME.
- Age at study initiation (mean and range, SD): 4 - 24 h
- Feeding during test: No feeding during test.

ACCLIMATION
- Acclimation conditions (same as test or not): The stock culture animals were held in batches of 30 - 50 adults at room temperature in ca. 1.8 L of dilution water for one week.
- Type and amount of food: The daphnids were fed daily with an algal suspension (Desmodesmus subspicatus) and Liquizell® (HOBBY). Algae growing in the log-phase were centrifuged and the pellet was re-suspended in a few mL of medium. Thirty millilitres of this suspension was given to 1 L of medium.
- Feeding frequency: Daily

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20.4 - 20.5 °C

pH:

Teststart: 7.74 - 8.50
Testend: 8.34 - 8.46

Dissolved oxygen:

Teststart: 8.6 - 8.9 mg/L
Testend: 7.6 - 7.9 mg/L

Nominal and measured concentrations:

nominal: 0, 6.25, 12.5, 25.0, 50.0 and 100 mg/L
geometric mean mesured: 0, 6.56, 13.0, 25.3, 52.4 aand 113 mg/L

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 60 mL glass beakers filled with approximately 50 mL of the test medium
- Aeration: no aeration
- Renewal rate of test solution (frequency/flow rate): no renewal as static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: copper-reduced dilution water

OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours light/darkness cycle
- Light intensity: 487 - 502 Lux

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: A non-GLP range-finding test was performed with the nominal loadings of 100 mg/L, 10 mg/L and 1.0 mg/L. Four replicates of the test organisms (five daphnids per replicate) were exposed to the test item for a period of 48 hours. No chemical analysis was performed.
- Results used to determine the conditions for the definitive study: Immobilisation of 10% and 100% was observed at the concentrations 10 and 100 mg/L, respectively.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 113 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

113 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Description of key information

LC50 (48 h) > 113 mg/L for Daphnia magna (according to OECD 202)

Additional information

The acute toxicity of 2 -isocyanatoethyl methacrylate (MOI) to invertebrates was tested in a study conducted by Showa Denko (2013), using daphnids (Daphnia magna) as test species. This static dose-response test was carried out according to the OECD-Guideline 202 under GLP-conditions over a period of 48 h. The nominal test concentrations used were 0, 6.25, 12.5, 25.0, 50.0 and 100 mg/L corresponding to mean measured concentrations of 0, 6.56, 13.0, 25.3, 52.4 and 113 mg/L. The resulting LC50 was >113 mg/L (geometric mean measured).