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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Directive 79/831/EEC, Annex V
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
For the concentration control analysis, samples from the controls and the highest test concentration were taken at 0 and 48 hours and analyzed by DOC determination.
Vehicle:
yes
Details on test solutions:
Cremophor RH 40, 100 mg/L
Test organisms (species):
Daphnia magna
Details on test organisms:
- Source: Institut National de Recherche Chimique Appliquée, France
- Age of the animals at the start of the test: 2-24 hours
- Age of stock animals: 2-4 weeks
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
Total hardness 2.63 mmol/L
Test temperature:
20.1-20.8 °C
pH:
7.9-8.0
Dissolved oxygen:
7.4-8.6 mg/L
Nominal and measured concentrations:
The test substance was tested in the following range of concentrations: 100 to 12.5 mg/L. In parallel, a control and a solvent control were run.
Details on test conditions:
- Test vessels: Tubes (glass) with flat bottom
- Volume of test vessels: Nominal 20 ml
- Test volume: 10 ml
- Number of animals/vessel: 5
- Total number of animals/conc.: 20
- Number of parallels/conc.: 4
- Number of parallels/control: 4
- Light: Artificial light, day/night-rhythm 16/8 hours

Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
50 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
24h EC0 > 100 mg/L
Summary of the concentration control analysis:
----------------------------------------------

 Time     Stocked with         Minimum           Maximum
 [h]       daphnids             [mg/L]            [mg/L]
-----------------------------------------------------------
  0           no                 42.8              42.8
 48           no                 50.0              50.0
 48           yes                41.5              41.5
-----------------------------------------------------------

The values given are the minimum and maximum concentrations of test  substance in the test solutions at various times of the study. The values are given in mg/L DOC and corrected for DOC of the solvent control.

Description of key information

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100 mg/L

Additional information