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Diss Factsheets
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EC number: 204-428-0 | CAS number: 120-82-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1982
- Reference Type:
- secondary source
- Title:
- European Union Risk Assessment Report - 1,2,4-Trichlorobenzene
- Author:
- European Commission - European Chemicals Bureau
- Year:
- 2 003
- Bibliographic source:
- Office for Official Publications of the European Communities
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- Analytical purity not reported. Acclimation period not reported. Body weights were not reported.
- GLP compliance:
- no
- Remarks:
- GLP was not mandatory at the time of the study
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- 1,2,4-trichlorobenzene
- EC Number:
- 204-428-0
- EC Name:
- 1,2,4-trichlorobenzene
- Cas Number:
- 120-82-1
- Molecular formula:
- C6H3Cl3
- IUPAC Name:
- 1,2,4-trichlorobenzene
- Details on test material:
- - Name of test material (as cited in study report): 1, 2, 4-trichlorobenzene
- Physical state: liquid
- Analytical purity: no data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Age at study initiation: 9 (males) - 14 (females) weeks
- Weight at study initiation: 159-173 g (males); 159-172 g (females)
- Fasting period before study: the animals were fasted from 16 to 4 hours before the dosing
- Housing: Rats were housed in number of 5 per cage in Makrolon cages type III
- Diet (e.g. ad libitum): Altromin R 1324, Altromin GmbH, Lage, Germany
- Water (e.g. ad libitum): tap water ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 1.5 °C
- Humidity (%): 60 ± 5%
- Photoperiod (hrs dark / hrs light): 12 hrs dark/ 12 hrs light (7am/7pm)
IN-LIFE DATES: From: April To: July 1981
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: cremophore/tap water mixture (1 : 4)
- Details on oral exposure:
- MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg
- Doses:
- 630-3100 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- other: not applicable
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: twice per day and once on weekends and holidays. The animals were weighed at the moment of the exposure, after 1 week, and at the end of the 14-day oberservation period.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathology analysis - Statistics:
- The calculation of the LD50 with the confidence interval for p <0.05 was performed with the Probit analysis according to Fink und Hund (Arzneim.-Forsch. 15:1965, 624)
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 930 mg/kg bw
- 95% CL:
- >= 840 - <= 1 050
- Remarks on result:
- other: Slope=9.11
- Mortality:
- The deaths occurred during the first 3 days of the treatment
- Clinical signs:
- other: See remark
- Body weight:
- other body weight observations
- Remarks:
- body weight was not reported
- Gross pathology:
- In the section of deceased male and female animals were noted alterations of the stomach and intestine mucous membrane, and gastric bleeding .
The liver had a localized clay colour and showed clear lobe markings. At the end of the test in the dissected male and female animals dosed with 1.0 and 1.25 mg/kg were observed slight alterations of the stomach. All other animals were examined macroscopically unremarkable.
Any other information on results incl. tables
Male |
||||
Dose mg/kg |
Number of dead animals |
Animals with symptoms |
Total number of animals employed |
Time of death |
630 |
0 |
0 |
5 |
|
730 |
1 |
5 |
5 |
2ndday |
800 |
3 |
5 |
5 |
2nd-3rdday |
1000 |
3 |
5 |
5 |
2ndday |
1250 |
5 |
5 |
5 |
24 hours-2ndday |
1600 |
5 |
5 |
5 |
24 hours |
3100 |
5 |
5 |
5 |
24 hours |
Female |
||||
Dose mg/kg |
Number of dead animals |
Animals with symptoms |
Total number of animals employed |
Time of death |
630 |
0 |
0 |
5 |
|
730 |
0 |
5 |
5 |
|
800 |
2 |
5 |
5 |
3rdday |
1000 |
3 |
5 |
5 |
2nd-3rdday |
1250 |
3 |
5 |
5 |
24 hours-2ndday |
1600 |
5 |
5 |
5 |
24 hours-2ndday |
3100 |
5 |
5 |
5 |
24 hours |
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Executive summary:
Groups of 5 rats of each sex with starting weights between 159 g and 173 g were given 1,2,4-trichlorobenzene (630-3100 mg/kg) by gavage according to OECD guideline 401 with deviations (Analytical purity not reported. Air changes not reported. Acclimation period not reported.Body weights not reported).
The LD50 was 930 mg/kg with 95% confidential limit of 840-1050.
The deaths occurred during the first 3 days of the treatment.
The oral single doses from 730 to 3100 mg/kg provoked the following poisoning symptoms: worsening of the general conditions, sedation, necrosis, side-prone position.
In the section of deceased male and female animals were noted alterations of the stomach and intestine mucous membrane, and gastric bleeding.
The liver had a localized clay colour and showed clear lobe markings. At the end of the test in the dissected male and female animals dosed with 1000 and 1250 mg/kg were observed slight alterations of the stomach. All other animals were examined macroscopically unremarkable.
On the basis of theses findings it is possible to classify 1,2,4 -trichlorobenzene in Category 4 (Acute Tox. 4, H302 according to GHS.
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