Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-428-0 | CAS number: 120-82-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction
Administrative data
- Endpoint:
- two-generation reproductive toxicity
- Remarks:
- based on test type (migrated information)
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable documented publication
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Multigeneration study of 1,2,4-trichlorobenzene in rats
- Author:
- Robinson KS, Kavlock RJ, Chernoff N, and Gray LE
- Year:
- 1 981
- Bibliographic source:
- J Toxicol Environ Health 8: 489 - 500
- Reference Type:
- secondary source
- Title:
- European Union Risk Assessment Report - 1,2,4-Trichlorobenzene
- Author:
- European Commission - European Chemicals Bureau
- Year:
- 2 003
- Bibliographic source:
- Office for Official Publications of the European Communities
Materials and methods
- Principles of method if other than guideline:
- Rats were continously exposed to 0, 25, 100, or 400 ppm 1,2,4-trichlorobenzene in the drinking water, beginning with birth of the F0 generation and continuing through weaning of the F2 generation. Fertility, growth, viability, locomotor activity, blood chemical analysis, and histopathology analysis were performed
- GLP compliance:
- no
- Remarks:
- GLP was not mandatory at the time of the study
- Limit test:
- no
Test material
- Reference substance name:
- 1,2,4-trichlorobenzene
- EC Number:
- 204-428-0
- EC Name:
- 1,2,4-trichlorobenzene
- Cas Number:
- 120-82-1
- Molecular formula:
- C6H3Cl3
- IUPAC Name:
- 1,2,4-trichlorobenzene
- Details on test material:
- Name of test material (as cited in study report): 1,2,4-trichlorobenzene
- Analytical purity: no data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- not specified
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratory, Wilmington, Mass, USA
- Age at study initiation: (P) 90-d-old; (F0) x 90-d-old; (F1) x 90-d-old
- Fasting period before study: no
- Housing: Upon arrival, 100 time-pregnant animals were housed individually in 43 X 23 X 20 cm plastic cages.
- Diet (e.g. ad libitum): Throughout the study, animals were fed commercial laboratory diet ad libitum.
- Water (e.g. ad libitum): The time-pregnant animals received tap water ad libitum until birth of F0 generation, when they were assigned to one of five treatment groups and given the appropriate water.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22-26 °C
- Photoperiod (hrs dark / hrs light): 12 h dark/ 12 h light)
Administration / exposure
- Route of administration:
- oral: drinking water
- Vehicle:
- other: the 1,2, 4-trichlorobenzene solutions contain 0.125% in drinking water.
- Details on exposure:
- Since 1,2,4-trichlorobenzene is not soluble in water at the concentrations used, the 1,2,4-trichlorobenzene solutions also contained a solubizer, Tween 20 at a concentraion of 0.125%.
Animals were given freshly prepared solutions at least twice weekley and allowed to drink ad libitum. - Details on mating procedure:
- - M/F ratio per cage: In F0 and F1 generations 1M/2F.
- Length of cohabitation: 2 weeks
- After successful mating each pregnant female was caged (how): individually - Analytical verification of doses or concentrations:
- no
- Duration of treatment / exposure:
- Exposure period: from birth of F0 generation through weaning of F2 generation
Premating exposure period (males): F0: 90 days; F1: 90 days
Premating exposure period (females): F0: 90 days; F1: 90 days
Duration of test: until day 32 of F2 generation - Frequency of treatment:
- continuously
- Details on study schedule:
- Age at mating of the mated animals in the study: 90 days
Doses / concentrations
- Remarks:
- Doses / Concentrations:
25, 100 or 400 mg/l (see remark)
Basis:
nominal in water
- No. of animals per sex per dose:
- In all generations, each treatment group consisted in 17-23 litters.
- Control animals:
- other: yes, concurrent no treatment; yes, concurrent vehicle
Examinations
- Statistics:
- The significance of treatment-related effects was analyzed by analysis of variance and, when results were significant, by linear regression. Whenever a significant dose response was found by linear regression, Dunn´s procedure was used to determine the significance of pairwise comparisons. The organ weight and blood chemistry data in the F0 and F1 generations at day 95 were analyzed by regression procedure as 2 x 2 x 4 (sex, generation, dose) factorial design.
Results and discussion
Results: P0 (first parental generation)
General toxicity (P0)
- Clinical signs:
- not examined
- Body weight and weight changes:
- effects observed, treatment-related
- Food consumption and compound intake (if feeding study):
- effects observed, treatment-related
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Histopathological findings: non-neoplastic:
- not examined
- Other effects:
- not examined
Reproductive function / performance (P0)
- Reproductive function: oestrous cycle:
- not examined
- Reproductive function: sperm measures:
- not examined
- Reproductive performance:
- no effects observed
Details on results (P0)
The postweaning growth of the F0 and F1 animals was not affected by 1,2,4-trichlorobenzene.
A treatment-related increase in food intake was found in the F0 males at day 29 (F [1,59]=4.97, p<0.028). There are also two instances of a treatment related decrease in water intake in the F0 generation, one in the females at day 35 (F [1,80]=5.71, p<0.018) and another in both sexes at day 83 [males, F [1,68]=11.72, p<0.001; females F [1,68]=15.05, p<0.001. In all three of these dietary effects in the F0 generation, Dunn´s procedure found only the high-dose group to be significantly different from controls. No treatment-related differenced in food or water intake was found in the F1 generation during the postweaning period.
ORGAN WEIGHTS (PARENTAL ANIMALS)
The 400 ppm 1,2,4-trichlorobenzene dose produced significantly increase enlarged adrenals in both sexes in the F0 and F1 animals at 95 d of age F [1,154]=7.68, p<0.006.
Effect levels (P0)
- Dose descriptor:
- NOAEL
- Effect level:
- ca. 25 mg/L drinking water
Results: F1 generation
General toxicity (F1)
- Clinical signs:
- not examined
- Mortality / viability:
- no mortality observed
- Body weight and weight changes:
- not examined
- Sexual maturation:
- no effects observed
- Organ weight findings including organ / body weight ratios:
- not examined
- Gross pathological findings:
- no effects observed
- Histopathological findings:
- not examined
Effect levels (F1)
- Dose descriptor:
- NOAEL
- Generation:
- F1
- Effect level:
- ca. 25 mg/L drinking water
Overall reproductive toxicity
- Reproductive effects observed:
- not specified
Any other information on results incl. tables
Table 1.Effects of 1, 2, 4-trichlorobenzene
|
Females delivering |
|||||||
|
Female bred |
Litter size,day 1 |
Percent mortality today 12 |
Percent mortality from d12 to weaning |
||||
Dose (ppm1,2,4-trichlorobenzene) |
F0 |
F1 |
F1 |
F2 |
F1 |
F2 |
F1 |
F2 |
0 (water) |
23/24 |
18/24 |
13.3±0.94a |
9.8±0.64 |
15.0±3.42 |
6.3±2.96 |
3.3±2.20 |
1.0±0.70 |
0 (tween20) |
22/24 |
19/24 |
12.8 ±0.71 |
10.3±0.87 |
14.2±3.46 |
9.3±5.62 |
1.2±0.92 |
3.6±2.74 |
25 |
19/24 |
21/24 |
14.5± 0.42 |
10.5±0.98 |
21.3±5.02 |
3.7±2.50 |
2.0±0.97 |
0.8±0.62 |
100 |
18/24 |
17/24 |
13.4±0.80 |
12.8±0.88 |
21.8±6.77 |
5.7±2.19 |
3.2±1.63 |
1.2±0.60 |
400 |
22/24 |
20/24 |
14.1±0.36 |
12.8±0.78 |
13.1±4.00 |
5.4±2.33 |
4.3±2.47 |
3.0±1.34 |
dValuesare means ± SE
Table 2. Representative Dietary Intake data from the F0 Generation with Calculated Dosages
Dose (ppm1,2,4-trichlorobenzene |
Female food intake (g/d per animal) |
Female water intake (g/d per animal) |
Calculated female dosage (mg/kg x day 1,2,4-trichlorobenzene) |
Male food intake (g/d per animal) |
Male water intake (mL/f per animal) |
Calculated male dosage (mg/kg x day 1,2,4-trichlorobenzene) |
29 days of age |
||||||
0 |
13.9±0.4a |
24.4±1.3 |
|
17.4±0.5 |
26.9±1.1 |
|
25 |
14.4±0.6 |
28.5±2.5 |
8.3±0.8 |
15.7±0.8 |
31.9±2.1 |
8.5±0.6 |
100 |
13.4±0.2 |
24.8±0.9 |
28.0±1.2 |
16.5±0.6 |
27.2±1.6 |
27.6±1.6 |
400 |
14.1±0.3 |
27.3±2.2 |
133.2±13.4 |
19.3±1.8b |
32.1 ±3.4 |
133.6±15.6 |
83 days of age |
||||||
0 |
20.0±0.7 |
31.6±1.6 |
|
31.7±0.8 |
43.9±1.8 |
|
25 |
20.0±0.7 |
31.6±1.3 |
3.7±0.1 |
30.6±1.0 |
47.0±2.2 |
2.5±0.1 |
100 |
20.0±0.8 |
30.8±1.7 |
14.8±1.0 |
30.9±1.2 |
42.0±1.3 |
8.9±0.3 |
400 |
19.6±0.8 |
26.2±0.8b |
53.6±3.9 |
29.3±0.6 |
38.6±1.7b |
33.0±1.4 |
aValues are means ± SE
bSignificantly different from control, p<0.05
Table 3. Locomotor Activity of Animals Exposed to 1,2,4-trichlorobenzene
|
|
|
Dose (ppm 1,2,4-trichlorobenzene) |
|||
Generation |
Age (days) |
Sex |
0 |
25 |
100 |
400 |
F0 |
16 |
M |
227±19.3a |
246±19.6 |
184±30.7 |
207±16.9 |
|
|
F |
292±44.2 |
233±36.2 |
202±35.7 |
253±34.3 |
F0 |
27 |
M |
418±36.8 |
390±40.0 |
459±31.5 |
428±19.6 |
|
|
F |
429±25.4 |
427±23.5 |
388±18.1 |
438±24.0 |
F0 |
48 |
M |
620±42.7 |
744±46.9 |
633±62.5 |
633±59.5 |
|
|
F |
701±37.7 |
778±86.7 |
772±38.5 |
811±61.4 |
F0 |
90 |
M |
611±70.9 |
678±44.0 |
556±69.4 |
772±70.0 |
|
|
F |
746±65.0 |
796±58.7 |
746±42.8 |
888±65.7 |
F1 |
31 |
M |
555±49.3 |
510±40.7 |
500±59.3 |
472±48.7 |
|
|
F |
467±63.2 |
556±40.7 |
462±51.3 |
578±48.7 |
F1 |
90 |
M |
611±46.7 |
590±110.9 |
596±45.0 |
586±55.7 |
|
|
F |
728±82.9 |
802±58.8 |
712±91.3 |
784±77.5 |
F2 |
26 |
M |
394±30.8 |
373±28.5 |
282±14.6 |
387±41.4 |
|
|
F |
286±44.2 |
359±88.5 |
344±58.1 |
373±70.0 |
Table 4.Effect of 1,2,4-trichlorobenzene in the drinking water on adrenal weights
Dose (ppm1,2,4-trichlorobenzene |
Males |
Females |
F0, 95 days |
||
0 (water) |
28.7±1.78a |
34.1±1.87 |
0 (tween20) |
28.6±1.09 |
36.8±1.14 |
25 |
28.8±1.59 |
35.6±1.70 |
100 |
28.2±1.10 |
36.6±1.08 |
400 |
31.8±2.43b |
41.5±1.99b |
F1, 95 days |
||
0 (water) |
27.1±1.71 |
35.8±1.89 |
0 (tween20) |
28.0±1.57 |
37.0±1.36 |
25 |
28.8±1.46 |
34.8±1.77 |
100 |
26.6±0.82 |
35.3±1.07 |
400 |
29.6±1.66b |
38.5±1.89b |
aValues are means ±SE; weights are in milligrams and for the left adrenal only-
bSignificantly different from controls, p<0.05
Applicant's summary and conclusion
- Executive summary:
Robinson (1981):
Rats were continuously exposed to 0, 25, 100, or 400 ppm 1,2,4-trichlorobenzene in the drinking water, beginning with birth of F0 generation and continuing through weaning of the F2 generation. The treatment did not affect fertility, growth, viability, locomotor activity, or blood chemical analyses. Adrenal gland enlargement was observed in both the F0 generation and F1 animals at 95 d of age.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.