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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Under the test conditions, no biodedegradation is observed as assessed in a CO2-Evolution and Closed Bottle Test according to OECD TG 301B and 301D, respectively.

Key value for chemical safety assessment

Additional information

The biodegradation potential of the substance in water was determined in a screening study according to OECD TG 301B (CO2 -Evolution Test).In this study 21 mg/L test substance was inoculated withactivated sludge for 28 days under aerobic conditions in the dark. During the incubation period degradation was followed by determining the carbon dioxide produced. After the 28 -day incubation period 8.5% of the test substance was biodegraded. The pass level of 60% degradation was was not met.

Additonally, the biodegradation potential was assessed in an OECD TG 301D (Closed Bottle Test) test. In this study 3 mg/L test substance was inoculated activated sludge for 28 days under aerobic conditions in the dark. After the 28-day incubation period, the cumulative percentages biodegradation were calculated from both the oxygen depletion (BOD/ThOD ratio) and from the decrease of test item concentrations. The observed biodegradation percentages were 24 and 25% in the two analysis methods. Overall, based on the findings of both tests the substance is classified as not readily biodegradable.