Butyl 2,3-epoxypropyl ether

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Principles of method if other than guideline:

To evaluate the acute percutaneous toxicity of the test substance, 2 male and 2 female rabbits per dose level were treated with 1000, 750, or 500 mg/kg bw.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Butyl 2,3-epoxypropyl ether

EC Number:

219-376-4

EC Name:

Butyl 2,3-epoxypropyl ether

Cas Number:

2426-08-6

Molecular formula:

C7H14O2

IUPAC Name:

2-(butoxymethyl)oxirane

Test material form:

liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Ray Nichols Rabbitry, Lumberton, Texas.
- Housing: Housed singly
- Diet: Commercial laboratory chow (Palston Purina Company, St Louis, Missouri), ad libitum.
- Water: Well water, ad libitum.
- Acclimation period: At least 1 week

ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12 / 12

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: The entire trunk was clipped free of hair with electric clippers.
- Type of wrap if used: The undiluted test material was applied under a heavy-gauge plastic sleeve held in place with rubber bands. The plastic sleeve was covered with a cloth bandage taped securely to the marginal hair.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): The sleeves were removed and the skins were washed with mild soap and water, rinsed thoroughly, and dried with a soft disposable towel.
- Time after start of exposure: After 24 hours

Duration of exposure:

24 hours

Doses:

1000, 750, and 500 mg/kg bw

No. of animals per sex per dose:

2

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The topical response at the test site of application was evaluated after removal of the plastic sleeve. The animals were observed frequently during exposure and for the following 2 weeks for signs of toxicity.
- Necropsy of survivors performed: At 2 weeks post-treatment, all surviving rabbits were submitted for gross pathological examination.

Statistics:

The acute percutaneous absorption LD50 was calculated by the moving average method as implemented in a computer program.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

778 mg/kg bw

Based on:

test mat.

95% CL:

>= 642 - <= 1 012

Mortality:

At 1000 mg/kg bw: 4/4 rabbits died.
At 750 mg/kg bw: 1/4 rabbits died.
At 500 mg/kg bw: 0/4 rabbits died.

Clinical signs:

other: Following treatment, the rabbits displayed signs of discomfort and became lethargic. The treated skin was necrotic with scabbing and scarring.

Gross pathology:

No internal lesions attributable to treatment were observed in the survivors upon gross pathological examination at 2 weeks.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria