C18 (branched) fatty acids, esters with mono-, di-, and tri-glycerol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980-07-25 to 1980-08-01

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Remarks:

Test follows accepted Guidelines available in 1980, no GLP, limited but sufficient reporting

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

C18 (branched) fatty acids, esters with mono-, di-, and tri-glycerol

EC Number:

701-479-5

Molecular formula:

C42H82O7

IUPAC Name:

C18 (branched) fatty acids, esters with mono-, di-, and tri-glycerol

Details on test material:

- Name of test material (as cited in study report): Diglycerol diisostearate
- Physical state: Viscous liquid, light yellow
- Analytical purity: 100 %

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Test concentrations nominal: 0-1-10-100-500 mg/L

Test solutions

Vehicle:

no

Details on test solutions:

Dispersion

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

L. idus from Paul Eggers, 2345 Hohenwestedt
10 fish, 1.4-2.5 g weight, 5.5-6.1 cm lenght

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

9.5 dH

Test temperature:

20 +/- 1 deg C

pH:

8.0-8.3

Dissolved oxygen:

7 mg/L

Salinity:

none

Nominal and measured concentrations:

nominal

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

At highest tested concentration of 500 mg/L nominal swim-up was observed after 24h exposure.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute fish toxicity of the registration substance was tested resulting in a NOEC (96h) of 100 mg/L nominal of the dispersion. Up to the highest tested concentration of 500 mg/L no mortality was observed. It is important to note that the water solubility of the test substance is 3 mg/L at 20 deg C and all except one concentration tested were above the water solubility.