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EC number: 219-440-1 | CAS number: 2437-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from authoritative databases.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- No data available
- Deviations:
- not specified
- Principles of method if other than guideline:
- Determination of acute short term toxicity Test of Dodecanenitrile on the growth of fish Oryzias latipes.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material: Dodecanenitrile
- Molecular formula: C12H23N
- Molecular weight: 181.321 g/mole
- Smiles notation: C(CCCCCC)CCCCC#N
- InChl: 1S/C12H23N/c1-2-3-4-5-6-7-8-9-10-11-12-13/h2-11H2,1H3
- Substance type: organic
- Physical state: colourless liquid - Analytical monitoring:
- yes
- Details on sampling:
- No data available
- Vehicle:
- not specified
- Details on test solutions:
- - Method: The test substance was added to the preparation vessel filled with test water to give 100 mg/L (nominal). The vessel was sealed and stirred gently for 48 hours, and then left standing for about 1 hour. After that, some amount of the solution in middle layer was extracted for stock solution to prepare test solutions by dilution of it.
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- - Feeding during test: No
- Test type:
- semi-static
- Water media type:
- other: Dechlorinated tap water
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Extended to 120 hours because of poor solubility of the test substance.
- Post exposure observation period:
- No data available
- Hardness:
- No data available
- Test temperature:
- 23.2 - 24.0 ◦C
- pH:
- No data available
- Dissolved oxygen:
- No data available
- Salinity:
- No data available
- Conductivity:
- No data available
- Nominal and measured concentrations:
- 100, 56, 31, 17, 9.5 % (geometric ratio 1.8) [1.3, 0.65, 0.35, 0.19, 0.096 mg/L as measured concentration], control
- Details on test conditions:
- TEST SYSTEM
- Test vessel: No data
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Test vessel filled with 5 liter of solution per concentration.
- Aeration: No
- Type of flow-through (e.g. peristaltic or proportional diluter): No data available
- Renewal rate of test solution (frequency/flow rate): Every 24 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): No data available
- Biomass loading rate: No data available
TEST MEDIUM / WATER PARAMETERS: No data available
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: Room light, 16 hours light / 8 hours dark
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : No data available
TEST CONCENTRATIONS: No data available
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.84 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Toxic to fishes
- Details on results:
- - Behavioural abnormalities: Mortality was observed at the concentration of 0.84 mg/l.
- Results with reference substance (positive control):
- No data available
- Reported statistics and error estimates:
- No data available
- Sublethal observations / clinical signs:
- Lowest concentration causing 100 %
mortality (96 h): 1.3 mg/L
- Highest concentration causing 0 % mortality (96 h): 0.35 mg/L,
(All results were values based on geometric mean values of the measured concentrations.)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the mortality of fish Oryzias latipes due to the exposure of Dodecanenitrile, the LC50 was 0.84 mg/l.
- Executive summary:
Determination of acute short term toxicity Test of Dodecanenitrile on the growth of fish Oryzias latipes. Test was performed according to the standard OECD guideline 203.
Various concentrations was prepared for the test is 0, 1.3, 0.65, 0.35, 0.19, 0.096 mg/L.The test substance was added to the preparation vessel filled with test water to give 100mg/L (nominal). The vessel was sealed and stirred gently for 48 hours, and then left standing for about 1 hour. After that, some amount of the solution in middle layer was extracted for stock solution to prepare test solutions by dilution of it. After the 96hrs of exposure with chemical mortality was observed.
Based on the mortality of fish Oryzias latipes due to the exposure of Dodecanenitrile, the LC50 was 0.84 mg/l. Thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.
Reference
Description of key information
Determination of acute short term toxicity Test of Dodecanenitrile on the growth of fish Oryzias latipes. Test was performed according to the standard OECD guideline 203.
Various concentrations was prepared for the test is 0, 1.3, 0.65, 0.35, 0.19, 0.096 mg/L.The test substance was added to the preparation vessel filled with test water to give 100mg/L (nominal). The vessel was sealed and stirred gently for 48 hours, and then left standing for about 1 hour. After that, some amount of the solution in middle layer was extracted for stock solution to prepare test solutions by dilution of it. After the 96hrs of exposure with chemical mortality was observed.
Based on the mortality of fish Oryzias latipes due to the exposure of Dodecanenitrile, the LC50 was 0.84 mg/l. Thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.84 mg/L
Additional information
Based on the various experimental data and prediction data for the target chemical study have been reviewed to determine the toxic nature of Dodecanenitrile (2437-25-4) on the growth of fishes. The studies are as mentioned below:
In the first experimental key study for Dodecanenitrile (2437-25-4) from j-check, 2017 Determination of acute short term toxicity Test of Dodecanenitrile on the growth of fish Oryzias latipes. Test was performed according to the standard OECD guideline 203. Various concentrations was prepared for the test is 0, 1.3, 0.65, 0.35, 0.19, 0.096 mg/L. The test substance was added to the preparation vessel filled with test water to give 100mg/L (nominal). The vessel was sealed and stirred gently for 48 hours, and then left standing for about 1 hour. After that, some amount of the solution in middle layer was extracted for stock solution to prepare test solutions by dilution of it. After the 96hrs of exposure with chemical mortality was observed. Based on the mortality of fish Oryzias latipes due to the exposure of Dodecanenitrile, the LC50 was 0.84 mg/l. Thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.
In the another experimental supporting study for Dodecanenitrile (2437-25-4), Toxicology Methods, 1997, The “gold standard” of fish toxicity testing is the 96 h flow-through fathead minnow (Pimephales promelas) 50% mortality database established by the U.S. Environmental Protection Agency. In the current study toxic potencies of chemicals measured in both tetratox and the 96-h Pimephales promelas lethality assay are compared. The Lethal concentration causing 50% mortality (LC50) of fathead minnow when exposed to Dodecanenitrile for 96 hours under the test conditions is 2.344 mg/l. It is reported to be bioactive.
Similarly in the third supporting study for the Dodecanenitrile (2437-25-4), ACD lab 2017, According to aquatic toxicity module i.e ACD/LabsI-Lab 2.0;Algorithm Version: v5.0.0.184 the lethal concentration (LC50) of the test substance Dodecanenitrile was predicted to be 0.28 mg/l on fish species Fathead minnow (Pimephales promelas). Based on the LC50 calculation the estimated Reliability Index (RI) of test chemical Dodecanenitrile predicted to be 0.28 mg/. As per model substance have moderate and high reliability ifRI > 0.5).Thus reported RII value indicate it has high reliability. And LC50 value indicate that the test substance Dodecanenitrile considered as toxic, harmful to aquatic organisms and thus can be classified as aquatic acute 1 and hazardous as per the CLP criteria.
In the fourth supporting predicted study for the Dodecanenitrile (2437-25-4), from EPIsuite, Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic fish was predicted. On the basis of this program, the LC 50 value for short term toxicity to aquatic fish was predicted to be 0.489 mg/l for Dodecanenitrile in 96 hrs. Based on this value it can be concluded that the substance Dodecanenitrile is considered to be toxic to aquatic environment and can be classified in aquatic acute 1/chronic 1 category as per the criteria mentioned in CLP regulation.
Similarly in the fifth supporting study for the Dodecanenitrile (2437-25-4), ecotox 2017, toxicity was measured. Determination of acute short term toxicity Test of Dodecanenitrile on the growth of Pimephales promelas (Fathead Minnow) was carried out for 96hrs. Test was performed in the static system by maintaining the proper temperature and Ph. After the 96hrs of exposure with chemical mortality and toxic symptoms was observed. Based on the mortality of fish Pimephales promelas (Fathead Minnow) due to the exposure of Dodecanenitrile, the LC50 was 0.43 mg/l. Thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.
Similarly in the sixth supporting study for Dodecanenitrile (2437-25-4) 2003, toxicity was measured. Determination of short term toxicity and mortality of Zebra fish (Brachydanio rerio) due to the exposure of Dodecanenitrile. Test was performed as per the standard OECD guidelines. 96hrs of exposure were given to the Zebra fishes by performing in static system. No fish died at 1 mg/l and 100 % of the fish died at 10 mg/l. Based on the mortality of Zebra fish (Brachydanio rerio) due to the exposure with Dodecanenitrile, the EC50 was > 1 mg/l. Based on the EC50 value it was concluded that the chemical was toxic and can be consider to be classified in aquatic chronic 2 as per the CLP classification criteria.
Based on the data obtain from various experimental studies and predicted study for the mortality of fishes due to the exposure of Dodecanenitrile thus it was concluded that the chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.
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