Dodecanenitrile

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from safety assessment report.

Data source

Reference

Reference Type:

review article or handbook

Title:

2,2'Azobis(2-Methylpropionitrile) - SIDS Initial Assessment Report

Author:

OECD SIDS -United Nations Environmental Programme (UNEP)

Year:

1999

Bibliographic source:

2,2'Azobis(2-Methylpropionitrile) - SIDS Initial Assessment Report; OECD SIDS; SIDS Initial Assessment Report For 9th SIAM (France, June 29-July 1, 1999)

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD TG 405 and EC TG 92/69/E.E.C., B5

Principles of method if other than guideline:

An ocular irritation study of test chemical 2,2'-Azobis(isobutyronitrile) (CAS No: 78-67-1) was carried out in New Zealand White rabbits in accordance with OECD TG 405 and EC TG 92/69/E.E.C., B5.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-Azobis(isobutyronitrile)

Cas Number:

78-67-1

Molecular formula:

C8H12N4

IUPAC Name:

2,2'-Azobis(isobutyronitrile)

Test material form:

solid

Details on test material:

- Name of test material: 2,2’-Azobis(2-methylpropionitrile)
- Molecular formula: C8H12N4
- Molecular weight: 164.2108 g/mol
- Substance type: Organic
- Physical state: solid
-Smiles: CC(C)(N=NC(C)(C)C#N)C#N
-InChI: 1S/C8H12N4/c1-7(2,5-9)11-12-8(3,4)6-10/h1-4H3/b12-11+

Specific details on test material used for the study:

- Name of test material: 2,2’-Azobis(2-methylpropionitrile)
- Molecular formula: C8H12N4
- Molecular weight: 164.2108 g/mol
- Substance type: Organic
- Physical state: solid
-Smiles: CC(C)(N=NC(C)(C)C#N)C#N
-InChI: 1S/C8H12N4/c1-7(2,5-9)11-12-8(3,4)6-10/h1-4H3/b12-11+

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

No data available

Test system

Vehicle:

not specified

Controls:

yes

Amount / concentration applied:

Single dose of 100 mg in original form

Duration of treatment / exposure:

72 hour

Observation period (in vivo):

After 1, 24, 48 and 72 hour

Duration of post- treatment incubation (in vitro):

No Data Available

Number of animals or in vitro replicates:

No Data Available

Details on study design:

TEST SITE
- Area of exposure: the conjunctival sac of
the left eye.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): The eyes were not rinsed

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

72 h

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No signs of ocular irritation were observed.

Applicant's summary and conclusion

Interpretation of results:

other: Not irritating

Conclusions:

The test material 2,2'-Azobis(isobutyronitrile) (CAS No: 78-67-1) was considered to be not irritating to the New Zealand White rabbits’ eye.

Executive summary:

An ocular irritation study of test chemical2,2'-Azobis(isobutyronitrile)(CAS No: 78-67-1) was carried out in New Zealand White rabbits in accordance with OECD TG 405 and EC TG 92/69/E.E.C., B5.

 

A single dose of 100 mg of 2,2'-azobis(2-methylpropanitrile) in original form of was administered into the conjunctival sac of the left eye by gently pulling the lower lid away from the eyeball while the right eye, which remained untreated, served as a control.

 

The eyes were not rinsed and examined approximately one hour, 24, 48 and 72 hours after administration.

 

The treated rabbits did not produce any ocular lesions. Hencethe chemical2,2'-Azobis(isobutyronitrile)(CAS No: 78-67-1)was considered to be not irritating to theNew Zealand White rabbits’ eye.