Dodecanenitrile

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from robust summary.

Qualifier:

according to guideline

Guideline:

other: As mention below

Version / remarks:

No data available

Deviations:

not specified

Principles of method if other than guideline:

Determination of short term toxicity and Acute Immobilization Test of Dodecanenitrile to Daphnia magna.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: Dodecanenitrile
- Molecular formula: C12H23N
- Molecular weight: 181.321 g/mole
- Smiles notation: C(CCCCCC)CCCCC#N
- InChl: 1S/C12H23N/c1-2-3-4-5-6-7-8-9-10-11-12-13/h2-11H2,1H3
- Substance type: organic
- Physical state: colourless liquid

Analytical monitoring:

not specified

Details on sampling:

No data available

Vehicle:

not specified

Details on test solutions:

No data available

Test organisms (species):

Daphnia sp.

Details on test organisms:

TEST ORGANISM
- Common name: Water flea

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Remarks on exposure duration:

No data available

Post exposure observation period:

No data available

Hardness:

No data available

Test temperature:

No data available

pH:

No data available

Dissolved oxygen:

No data available

Salinity:

No data available

Conductivity:

No data available

Nominal and measured concentrations:

No data available

Details on test conditions:

No data available

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.33 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Immobility was observed

Details on results:

No data available

Results with reference substance (positive control):

No data available

Reported statistics and error estimates:

No data available

Validity criteria fulfilled:

not specified

Conclusions:

Based on the immobilization of daphnia due to the exposure with chemical Dodecanenitrile, the LC50 was 0.33 mg/l for 48hrs.

Executive summary:

Determination of short term toxicity and Acute Immobilization Test of Dodecanenitrile to Daphnia species. Study was performed in static system for 48hrs exposure of chemical Dodecanenitrile to daphnia.

 

Based on the immobilization of daphnia due to the exposure with chemical Dodecanenitrile, the LC50 was 0.33 mg/l for 48hrs. Thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.

Description of key information

Determination of short term toxicity and Acute Immobilization Test of Dodecanenitrile to Daphnia magna. Test was performed according to the OECD guidelines in the semi-static system for 48hrs. 

 

For the study dechlorinated water were used. Various concentrations was prepared 0.73, 0.36, 0.16, 0.078, 0.035 mg/L as measured concentration, one control was also used. The test solution prepared by adding test substance and test water were mixed to give 100 mg/L (nominal), and stirred for about 48 hours. Some amount of solution in middle layer was extracted for stock solution to prepare test solutions by mixing and stirring with test water.

 

Based on the immobilization of daphnia magna due to the exposure with chemical Dodecanenitrile, the EC50 was 0.059 mg/l for 48hrs. thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.059 mg/L

Additional information

Based on the various experimental data and prediction data for the target chemical study have been reviewed to determine the toxic nature of Dodecanenitrile (2437-25-4) on the growth of fishes. The studies are as mentioned below:

In the first experimental key study for Dodecanenitrile (2437-25-4) from j-check, 2017 Determination of short term toxicity and Acute Immobilization Test of Dodecanenitrile to Daphnia magna. Test was performed according to the OECD guidelines in the semi-static system for 48hrs. For the study dechlorinated water were used. Various concentrations was prepared 0.73, 0.36, 0.16, 0.078, 0.035 mg/L as measured concentration, one control was also used. The test solution prepared by adding test substance and test water were mixed to give 100 mg/L (nominal), and stirred for about 48 hours. Some amount of solution in middle layer was extracted for stock solution to prepare test solutions by mixing and stirring with test water. Based on the immobilization of daphnia magna due to the exposure with chemical Dodecanenitrile, the EC50 was 0.059 mg/l for 48hrs. thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.

 

Similarly in the second supporting study for Dodecanenitrile (2437-25-4) episuite, 2017, toxicity was measured. Based on the prediction done by EPI suite, ECOSAR version 1.1, on the basis of similarity of structure to chemicals for which the aquatic toxicity has been previously measured by structure-activity relationships (SARs) program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted. On the basis of this program, the LC 50 value for short term toxicity to aquatic invertebrates was predicted to be 0.358 mg/l for Dodecanenitrile in 48 hrs. Based on this value it can be concluded that the substance Dodecanenitrile is considered to be toxic to aquatic environment and can be classified in aquatic acute 1 category as per the criteria mentioned in CLP regulation.

 

Similarly in the third supporting study for Dodecanenitrile (2437-25-4) 2003, toxicity was measured. Determination of short term toxicity and Acute Immobilization Test of Dodecanenitrile to Daphnia species. Study was performed in static system for 48hrs exposure of chemical Dodecanenitrile to daphnia. Based on the immobilization of daphnia due to the exposure with chemical Dodecanenitrile, the LC50 was 0.33 mg/l for 48hrs. Thus chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.

Similarly in the fourth supporting study for RA chemical 1-Octanethiol (111-88-6), j-check, Determination of short term toxicity of 1-Octanethiol on the mobility of daphnia was studied by providing the exposure of chemical for 48hrs. Test was performed by following the standard OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). After the 48hrs of exposure interval period, toxicity and immobilisation was observed. Based on the inhibition of mobility of daphnia species due to the exposure of chemical 1-Octanethiol, the EC50 was 0.024 mg/l. Thus based on the EC50, chemical 1-Octanethiol was consider as toxic and can be consider to be classified as aquatic acute 1 category as per the CLP classification criteria.

 

 

In the same manner by considering fifth supporting study for the RA chemical 1-Hexanethiol (111 -31 -9), from ecotox databases 2017, Evaluation of short term toxicity of 1-Hexanethiol studied on the growth of daphnia magna by providing the exposure period of 24hrs. Test performed in static system. After the exposure effect concentration was observed on the basis of behavioral changes in daphnia magna and measure effect by the equilibrium. Based on the behavior of daphnia magna after the exposure of chemical 1-Hexanethiol for 24hrs, the EC50 was 0.073 mg/l. Thus chemical 1-Hexanethiol was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.

Based on the data obtain from various experimental studies for the immobility due to the exposure of Dodecanenitrile thus it was concluded that the chemical Dodecanenitrile was consider as toxic and can be consider to be classified as aquatic acute 1 category as per CLP classification criteria.